Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,203,390	A→G	L248L (TTG→CTG)	bacA ←	undecaprenyl pyrophosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,203,390	0	A	G	100.0%	146.1 / NA	41	L248L (TTG→CTG)	bacA	undecaprenyl pyrophosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/17);  total (24/17)

ATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTGCGCCCATC  >  NC_000913/3203258‑3203520                                                                                                                                     |                                                                                                                                   aTTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAgg                                                                                                                             <  2:310567/140‑1 (MQ=255)        cTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTAAATAAAAAGAACACGACATACCCCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGttt                                                                                                                      <  1:61176/140‑1 (MQ=255)              aaTCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACCCCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCg                                                                                                                <  2:781286/140‑1 (MQ=255)                                     cGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCg                                                                                         <  1:386445/140‑1 (MQ=255)                                                  cAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCaa                                                                            >  2:402864/1‑140 (MQ=255)                                                         aaGAACACGACATCCCCCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCaaa                                                                     <  1:455251/140‑1 (MQ=255)                                                             acacGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATc                                                                 >  1:76926/1‑140 (MQ=255)                                                               acGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCgg                                                               >  1:338449/1‑140 (MQ=255)                                                               acGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCgg                                                               >  1:94407/1‑140 (MQ=255)                                                                   cATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGata                                                           >  2:710628/1‑140 (MQ=255)                                                                      acacCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATcg                                                        >  1:212418/1‑140 (MQ=255)                                                                       cacCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATcgc                                                       >  1:645409/1‑140 (MQ=255)                                                                         ccGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATcgccg                                                     >  1:635167/1‑140 (MQ=255)                                                                         ccGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATcgccg                                                     <  2:212418/140‑1 (MQ=255)                                                                           gCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCt                                                   >  2:456039/1‑140 (MQ=255)                                                                             aGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCg                                                                >  2:788318/1‑125 (MQ=255)                                                                             aGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCg                                                                <  1:788318/125‑1 (MQ=255)                                                                             aGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTg                                                 >  2:799250/1‑140 (MQ=255)                                                                             aGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTg                                                 >  1:215712/1‑140 (MQ=255)                                                                             aGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTg                                                 >  2:836039/1‑140 (MQ=255)                                                                                  ccACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAgg                                            <  2:94407/140‑1 (MQ=255)                                                                                         aaaGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGcccc                                     >  1:794033/1‑140 (MQ=255)                                                                                           aGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAg                                   <  2:645409/140‑1 (MQ=255)                                                                                             cGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCt                                 >  1:98660/1‑140 (MQ=255)                                                                                             cGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCt                                 >  1:414255/1‑140 (MQ=255)                                                                                             cGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCt                                 >  1:6838/1‑140 (MQ=255)                                                                                              gATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCtt                                <  2:215712/140‑1 (MQ=255)                                                                                                tAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCtttt                              >  2:314945/1‑140 (MQ=255)                                                                                                       cGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAgaga                       >  1:619729/1‑140 (MQ=255)                                                                                                       cGAACGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAgaga                       >  2:816686/1‑140 (MQ=255)                                                                                                         aaCGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTag                          >  1:648198/1‑135 (MQ=255)                                                                                                         aaCGGGATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTag                          <  2:648198/135‑1 (MQ=255)                                                                                                            gggATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTTTCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAg                  <  2:98660/140‑1 (MQ=255)                                                                                                            gggATAAACGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAg                  <  2:414255/140‑1 (MQ=255)                                                                                                                 aaaCGAAATGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCg             <  1:456039/140‑1 (MQ=255)                                                                                                                      aaaTGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTgcg        >  1:590850/1‑140 (MQ=255)                                                                                                                       aaTGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTgcgc       <  2:6838/140‑1 (MQ=255)                                                                                                                        aTGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTgcgc       >  2:213161/1‑139 (MQ=255)                                                                                                                        aTGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTgcgc       <  1:213161/139‑1 (MQ=255)                                                                                                                        aTGCGCTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTGCGcc      >  1:345478/1‑140 (MQ=255)                                                                                                                          gcgcTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTGCGCCca    <  2:619729/140‑1 (MQ=255)                                                                                                                            gcTTAATCAGTTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTGCGCCcatc  <  2:635167/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   ATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTGCGCCCATC  >  NC_000913/3203258‑3203520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑