Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	366,162	A→G	P48P (CCT→CCC)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	366,162	0	A	G	100.0%	130.4 / NA	37	P48P (CCT→CCC)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/23);  total (14/23)

TGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATC  >  NC_000913/366025‑366297                                                                                                                                          |                                                                                                                                        tGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGACAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAggg                                                                                                                                       >  2:767254/1‑140 (MQ=255) tGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTGTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAggg                                                                                                                                       >  2:756982/1‑140 (MQ=255)  gCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAgggg                                                                                                                                      >  2:572828/1‑140 (MQ=255)      cTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGAt                                                                                                                                  <  1:780059/140‑1 (MQ=255)      cTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGAt                                                                                                                                  <  2:685268/140‑1 (MQ=255)      cTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGAt                                                                                                                                  <  2:677788/140‑1 (MQ=255)      cTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGAt                                                                                                                                  >  1:677788/1‑140 (MQ=255)             tttGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCg                                                                                                                           <  1:572828/140‑1 (MQ=255)               tGACGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGAt                                                                                                                                  <  1:810042/131‑1 (MQ=255)               tGACGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGAt                                                                                                                                  >  2:810042/1‑131 (MQ=255)                gAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGc                                                                                                                        <  1:590027/140‑1 (MQ=255)                gAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGc                                                                                                                        <  1:756982/140‑1 (MQ=255)                 aGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAggg                                                                                                                                       >  2:796956/1‑124 (MQ=255)                        gacgacAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGGCTGTTCGc                                                                                                                <  2:635471/140‑1 (MQ=255)                                  tCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACg                                                                                                         >  1:180400/1‑137 (MQ=255)                                  tCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACg                                                                                                         <  2:180400/137‑1 (MQ=255)                                    ggCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGc                                                                                                    <  2:42419/140‑1 (MQ=255)                                    ggCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGc                                                                                                    >  1:42419/1‑140 (MQ=255)                                    ggCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGc                                                                                                    <  1:275255/140‑1 (MQ=255)                                          aGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCACTATTACGCCAGCTGGCGa                                                                                              >  1:819274/1‑140 (MQ=255)                                                        ccagccagCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGtgct                                                                                >  2:3476/1‑140 (MQ=255)                                                            ccagcTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCACTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCaa                                                                            <  2:819274/140‑1 (MQ=255)                                                              agcTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAgg                                                                          >  2:325505/1‑140 (MQ=255)                                                                 tttCCGGCACCGCTTCTGGTGCCGGAAACCAGACAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGa                                                                       <  1:767254/140‑1 (MQ=255)                                                                           cGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTggg                                                             >  2:646612/1‑140 (MQ=255)                                                                            gCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGt                                                            <  1:200733/140‑1 (MQ=255)                                                                            gCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGt                                                            <  1:439356/140‑1 (MQ=255)                                                                                            aCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTcc                                            <  1:325505/140‑1 (MQ=255)                                                                                                 gCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCa                                                     <  1:688542/126‑1 (MQ=255)                                                                                                 gCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCa                                                     >  2:688542/1‑126 (MQ=255)                                                                                                 gCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTc                                       >  1:274377/1‑140 (MQ=255)                                                                                                 gCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTc                                       >  1:262511/1‑140 (MQ=255)                                                                                                     aGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCacga                                   <  2:629688/140‑1 (MQ=255)                                                                                                                    tcAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAacgacg                    >  1:714291/1‑140 (MQ=255)                                                                                                                    tcAGGCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGACGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAacgacg                    <  2:756048/140‑1 (MQ=255)                                                                                                                       ggCTGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGcc                 <  1:3476/140‑1 (MQ=255)                                                                                                                          tGCGCAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGt              >  1:725280/1‑140 (MQ=255)                                                                                                                           gcgcAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTg             >  1:685923/1‑140 (MQ=255)                                                                                                                            cgcAACTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGa            <  1:646612/140‑1 (MQ=255)                                                                                                                                 cTGTTGGGAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCg       <  2:714291/140‑1 (MQ=255)                                                                                                                                      gggAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATc  <  2:345401/140‑1 (MQ=255)                                                                                                                                      gggAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATc  <  2:274377/140‑1 (MQ=255)                                                                                                                                      gggAGGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATc  <  2:262511/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TGCATCTGCCAGTTTGAGGGGACGACGACAGTATCGGCCTCAGGAAGATCGCACTCCAGCCAGCTTTCCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATC  >  NC_000913/366025‑366297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑