Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,568,390	T→C	L313L (TTA→TTG)	glgC ←	glucose‑1‑phosphate adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,568,390	0	T	C	100.0%	223.4 / NA	62	L313L (TTA→TTG)	glgC	glucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/34);  total (28/34)

AATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCA  >  NC_000913/3568253‑3568522                                                                                                                                          |                                                                                                                                     aatgaatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCaa                                                                                                                                    >  1:289090/1‑140 (MQ=255)    gaatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGa                                                                                                                                 <  2:91267/140‑1 (MQ=255)      atTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGAtt                                                                                                                               <  2:197377/140‑1 (MQ=255)      atTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGAtt                                                                                                                               <  1:472692/140‑1 (MQ=255)       ttCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTc                                                                                                                              <  2:316372/140‑1 (MQ=255)           cgcgAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTg                                                                                                                          >  2:618733/1‑140 (MQ=255)           cgcgAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTg                                                                                                                          <  1:673831/140‑1 (MQ=255)             cgAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTa                                                                                                                        >  2:186094/1‑140 (MQ=255)                     cgAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGaa                                                                                                                <  2:110358/140‑1 (MQ=255)                              aacGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAAt                                                                                                       <  1:169514/140‑1 (MQ=255)                               acGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAAtt                                                                                                      <  2:289090/140‑1 (MQ=255)                                cGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTg                                                                                                     <  1:85757/140‑1 (MQ=255)                                cGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTg                                                                                                     <  1:116468/140‑1 (MQ=255)                                   aCTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGa                                                                                                  <  1:435433/140‑1 (MQ=255)                                    cTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGAt                                                                                                 <  2:614769/140‑1 (MQ=255)                                    cTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGAt                                                                                                 <  1:332964/140‑1 (MQ=255)                                         ccaccaccGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTAc                                                                                            <  1:775057/140‑1 (MQ=255)                                            ccaccGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACata                                                                                         >  2:167560/1‑140 (MQ=255)                                             caccGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACatat                                                                                        <  2:275808/140‑1 (MQ=255)                                                  aaCCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGt                                                                                   >  1:371136/1‑140 (MQ=255)                                                  aaCCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGt                                                                                   >  2:594069/1‑140 (MQ=255)                                                  aaCCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGt                                                                                   >  1:275193/1‑140 (MQ=255)                                                       gagaTCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCgg                                                                              <  1:255971/140‑1 (MQ=255)                                                         gaTCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGca                                                                            <  1:640180/140‑1 (MQ=255)                                                         gaTCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGca                                                                            <  1:173373/140‑1 (MQ=255)                                                            cacacaACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGcacca                                                                         >  1:734812/1‑140 (MQ=255)                                                             acacaACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGcaccac                                                                        >  1:800153/1‑140 (MQ=255)                                                              cacaACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACcaca                                                                       <  2:734812/140‑1 (MQ=255)                                                              cacaACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACcaca                                                                       >  1:227208/1‑140 (MQ=255)                                                              cacaACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACcaca                                                                       <  1:186094/140‑1 (MQ=255)                                                                    cgccgGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGcc                                                                 >  2:558388/1‑140 (MQ=255)                                                                    cgccgGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGcc                                                                 <  2:329066/140‑1 (MQ=255)                                                                      ccgGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAg                                                               >  2:247157/1‑140 (MQ=255)                                                                      ccgGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAg                                                               >  1:227906/1‑140 (MQ=255)                                                                      ccgGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAg                                                               >  1:827973/1‑140 (MQ=255)                                                                      ccgGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAg                                                               >  2:467807/1‑140 (MQ=255)                                                                      ccgGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAg                                                               >  1:639704/1‑140 (MQ=255)                                                                      ccgGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAg                                                               >  2:806411/1‑140 (MQ=255)                                                                        ggAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGAt                                                             >  1:369508/1‑140 (MQ=255)                                                                          aaaCCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCg                                                           <  2:10280/140‑1 (MQ=255)                                                                              cAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGt                                                       <  1:247157/140‑1 (MQ=255)                                                                              cAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGt                                                       <  2:34907/140‑1 (MQ=255)                                                                                    gTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCtt                                                 >  2:451922/1‑140 (MQ=255)                                                                                       aaGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTcc                                              <  2:827973/140‑1 (MQ=255)                                                                                          ggTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGt                                           <  1:806411/140‑1 (MQ=255)                                                                                             cATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAg                                        >  1:272854/1‑140 (MQ=255)                                                                                               tCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCt                                      >  1:451198/1‑140 (MQ=255)                                                                                               tCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCt                                      >  1:6919/1‑140 (MQ=255)                                                                                                cccGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCtt                                     <  2:227208/140‑1 (MQ=255)                                                                                                  cGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTcc                                   <  2:272854/140‑1 (MQ=255)                                                                                                         aCCGGAGCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTAc                            >  2:116538/1‑140 (MQ=255)                                                                                                               gCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACAt                      <  1:116538/140‑1 (MQ=255)                                                                                                               gCGATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACAt                      <  2:6919/140‑1 (MQ=255)                                                                                                                 gATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTc                                    <  2:621383/124‑1 (MQ=255)                                                                                                                 gATCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTc                                    >  1:621383/1‑124 (MQ=255)                                                                                                                  aTCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATcgc                   <  1:451922/140‑1 (MQ=255)                                                                                                                  aTCCTGCACGAATTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATcgc                   >  1:346892/1‑140 (MQ=255)                                                                                                                             aTTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGc        >  2:51362/1‑140 (MQ=255)                                                                                                                             aTTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGc        >  2:853813/1‑140 (MQ=255)                                                                                                                             aTTTCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGc        >  2:361988/1‑140 (MQ=255)                                                                                                                                tCGCTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCg     <  2:371136/140‑1 (MQ=255)                                                                                                                                   cTGGCGGCAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCa  <  1:467807/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     AATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGTTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCA  >  NC_000913/3568253‑3568522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑