Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,758,973	C→T	V297I (GTT→ATT)	selB ←	selenocysteyl‑tRNA‑specific translation elongation factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,758,973	0	C	T	100.0%	128.6 / NA	38	V297I (GTT→ATT)	selB	selenocysteyl‑tRNA‑specific translation elongation factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/21);  total (17/21)

AAGCATCACGACGCGCGCTCCGGCCAGCGTGTTGCGGGCAGAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAACGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCACCCGTGTGAACGGCTCTGGCGGCACATCGGCAAGCAGCCAGTCGC  >  NC_000913/3758842‑3759104                                                                                                                                    |                                                                                                                                    aaGCATCACGACGCGCGCTCCGGCCAGCGTGTTGCGGGCAGAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCg                                                                                                                             >  2:580835/1‑140 (MQ=255)     aTCACGACGCGCGCTCCGGCCAGCGTGTTGCGGGCAGAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGAc                                                                                                                         <  1:861918/140‑1 (MQ=255)            cgcgcgCTCCGGCCAGCGTGTTGCGGGCAGAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGtggctg                                                                                                                  >  2:11036/1‑140 (MQ=255)                 gcTCCGGCCAGCGTGTTGCGGGCAGAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTggcggc                                                                                                             <  2:100557/140‑1 (MQ=255)                           gCGTGTTGCGGGCAGAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAata                                                                                                   >  2:802759/1‑140 (MQ=255)                           gCGTGTTGCGGGCAGAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAata                                                                                                   <  2:530960/140‑1 (MQ=255)                               gtTGCGGGCAGAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCa                                                                                               <  1:507179/140‑1 (MQ=255)                                 tGCGGGCAGAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGgcggcg                                                                                                            <  1:436642/125‑1 (MQ=255)                                 tGCGGGCAGAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGgcggcg                                                                                                            >  2:436642/1‑125 (MQ=255)                                   cGGGCAGAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCgg                                                                                           <  1:11036/140‑1 (MQ=255)                                   cGGGCAGAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCgg                                                                                           <  2:142632/140‑1 (MQ=255)                                                cgcgCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCa                                                                                     <  2:612922/133‑1 (MQ=255)                                                cgcgCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCa                                                                                     >  1:612922/1‑133 (MQ=255)                                                  cgcAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGt                                                                               <  1:477804/137‑1 (MQ=255)                                                  cgcAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGt                                                                               >  2:477804/1‑137 (MQ=255)                                                  cgcAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAg                                                                            >  2:440880/1‑140 (MQ=255)                                                  cgcAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAg                                                                            <  2:397986/140‑1 (MQ=255)                                                  cgcAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAg                                                                            <  2:298823/140‑1 (MQ=255)                                                  cgcAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAg                                                                            <  2:471419/140‑1 (MQ=255)                                                  cgcAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAg                                                                            <  1:304310/140‑1 (MQ=255)                                                  cgcAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAg                                                                            <  1:32494/140‑1 (MQ=255)                                                  cgcAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAg                                                                            >  1:143163/1‑140 (MQ=255)                                                             gCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAg                                                                            <  2:601449/129‑1 (MQ=255)                                                             gCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAg                                                                            >  1:601449/1‑129 (MQ=255)                                                                           ccagccaTAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACa                                                   >  2:307982/1‑140 (MQ=255)                                                                             agccaTAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAAt                                                 <  1:487970/140‑1 (MQ=255)                                                                               ccaTAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCa                                               >  1:265222/1‑140 (MQ=255)                                                                                         gtgtCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCACCCGTGTGaa                                     <  1:580835/140‑1 (MQ=255)                                                                                          tgtCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCACCCGTGTGaa                                     >  2:172980/1‑139 (MQ=255)                                                                                          tgtCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCACCCGTGTGaa                                     <  1:172980/139‑1 (MQ=255)                                                                                                aGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCACCCGTGTGAACGGctct                              >  2:646567/1‑140 (MQ=255)                                                                                                       ttCAGCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCACCCGTGTGAACGGCTCTGGCGGca                       >  2:14464/1‑140 (MQ=255)                                                                                                           gCAAGGTTATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCACCCGTGTGAACGGCTCTGGCGGCACATc                   <  2:560335/140‑1 (MQ=255)                                                                                                                 ttATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCACCCGTGTGAACGGCTCTggcg                          <  1:738574/127‑1 (MQ=255)                                                                                                                 ttATCTTCCAGCAGTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCACCCGTGTGAACGGCTCTggcg                          >  2:738574/1‑127 (MQ=255)                                                                                                                          agcagTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCACCCGTGTGAACGGCTCTGGCGGCACATCGGCAAGCAGCCAGTc    >  1:559047/1‑140 (MQ=255)                                                                                                                           gcagTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCACCCGTGTGAACGGCTCTGGCGGCACATCGGCAAGCAGCCAGTCg   >  1:387303/1‑140 (MQ=255)                                                                                                                            cagTGAAATGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCACCCGTGTGAACGGCTCTGGCGGCACATCGGCAAGCAGCCAGTCGc  <  1:646567/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    AAGCATCACGACGCGCGCTCCGGCCAGCGTGTTGCGGGCAGAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAACGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCACCCGTGTGAACGGCTCTGGCGGCACATCGGCAAGCAGCCAGTCGC  >  NC_000913/3758842‑3759104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑