Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,760,826	T→C	T142A (ACG→GCG)	selA ←	selenocysteine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,760,826	0	T	C	100.0%	176.1 / NA	49	T142A (ACG→GCG)	selA	selenocysteine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/28);  total (21/28)

ATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTC  >  NC_000913/3760693‑3760946                                                                                                                                      |                                                                                                                         aTACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACg                                                                                                                    >  1:590347/1‑140 (MQ=255)    cGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCaa                                                                                                                        >  1:721324/1‑133 (MQ=255)    cGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCaa                                                                                                                        <  2:721324/133‑1 (MQ=255)    cGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATAc                                                                                                                     <  1:745191/136‑1 (MQ=255)    cGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATAc                                                                                                                     >  2:745191/1‑136 (MQ=255)          gcgcCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCAcagcag                                                                                                           >  2:547583/1‑140 (MQ=255)          gcgcCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCAcagcag                                                                                                           >  2:270576/1‑140 (MQ=255)                     ctcCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAgcg                                                                                                >  1:827865/1‑140 (MQ=255)                          ccaGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGa                                                                                           >  2:304875/1‑140 (MQ=255)                                  ccgcGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATg                                                                                   >  2:51716/1‑140 (MQ=255)                                  ccgcGAGATACCACCACCTCTTTTCCGCTGGCAGTGACCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCACAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATg                                                                                   >  2:240796/1‑140 (MQ=255)                                     cgAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTcc                                                                                <  2:827865/140‑1 (MQ=255)                                     cgAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTcc                                                                                <  1:51716/140‑1 (MQ=255)                                       agaTACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCcgc                                                                              >  1:841732/1‑140 (MQ=255)                                               ccaccTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGcg                                                                      <  1:358621/140‑1 (MQ=255)                                                  ccTCTTTTCCGCTGGCAGTGGCCACCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGcgtcg                                                                   >  2:412264/1‑140 (MQ=255)                                                   ctctTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGcgtcgt                                                                  <  1:770928/140‑1 (MQ=255)                                                    tctTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCgtcgtc                                                                 <  2:380938/140‑1 (MQ=255)                                                    tctTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCgtcgtc                                                                 <  1:547583/140‑1 (MQ=255)                                                    tctTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCgtcgtc                                                                 <  1:270576/140‑1 (MQ=255)                                                    tctTTTCCGCTGGCAGTGACCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCACAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCgtcgtc                                                                 <  1:240796/140‑1 (MQ=255)                                                          ccGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATc                                                           <  2:841732/140‑1 (MQ=255)                                                          ccGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATc                                                           >  1:597619/1‑140 (MQ=255)                                                            gCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCAt                                                         <  2:186107/140‑1 (MQ=255)                                                            gCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCAt                                                         <  2:597619/140‑1 (MQ=255)                                                              tGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCTCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATAc                                                       <  2:67945/140‑1 (MQ=255)                                                                gCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTc                                                     >  2:389327/1‑140 (MQ=255)                                                                 cAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCg                                                    <  1:674651/140‑1 (MQ=255)                                                                 cAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCg                                                    <  1:65140/140‑1 (MQ=255)                                                                             aCATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGc                                        <  1:185642/140‑1 (MQ=255)                                                                              cATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCg                                       <  2:827234/140‑1 (MQ=255)                                                                              cATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCg                                       <  1:304875/140‑1 (MQ=255)                                                                              cATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCg                                       <  1:412264/140‑1 (MQ=255)                                                                                      acacCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATa                               >  2:431709/1‑140 (MQ=255)                                                                                       cacCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCa                                             >  2:28426/1‑125 (MQ=255)                                                                                       cacCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCa                                             <  1:28426/125‑1 (MQ=255)                                                                                        cccgccgccgCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGc                             <  2:830168/139‑1 (MQ=255)                                                                                          cgccgccgcATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCt                           >  2:100801/1‑140 (MQ=255)                                                                                            ccgccgcATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTgc                         >  1:506094/1‑140 (MQ=255)                                                                                            ccgccgcATTGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTgc                         >  2:621535/1‑140 (MQ=255)                                                                                                    ttGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCa                      <  1:574939/135‑1 (MQ=255)                                                                                                    ttGTTATTGACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCa                      >  2:574939/1‑135 (MQ=255)                                                                                                            gACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACcg         <  2:590347/140‑1 (MQ=255)                                                                                                            gACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACcg         <  1:621535/140‑1 (MQ=255)                                                                                                            gACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACcg         <  1:100801/140‑1 (MQ=255)                                                                                                            gACGATACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCACGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACcg         >  1:235834/1‑140 (MQ=255)                                                                                                                 tACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCt    <  2:212124/140‑1 (MQ=255)                                                                                                                 tACAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCt    >  1:654008/1‑140 (MQ=255)                                                                                                                   cAGGCATCTTCCGCCCCCGCAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTc  <  1:686307/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                         ATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTC  >  NC_000913/3760693‑3760946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑