| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,773,405 | C→T | S375S (AGC→AGT) | mtlA → | mannitol‑specific PTS enzyme IICBA component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,773,405 | 0 | C | T | 96.6% | 193.4 / ‑2.8 | 59 | S375S (AGC→AGT) | mtlA | mannitol‑specific PTS enzyme IICBA component |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (25/32); total (26/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGC > NC_000913/3773273‑3773541 | tATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCacgtac < 2:396475/140‑1 (MQ=255) tttGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCAcgtacgta > 1:242327/1‑140 (MQ=255) gCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTaaaa < 1:108262/140‑1 (MQ=255) gAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAatca < 1:41474/140‑1 (MQ=255) aaGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCtgt < 1:315435/140‑1 (MQ=255) gTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGa < 1:686449/140‑1 (MQ=255) agaagaagaTGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCg < 2:325518/140‑1 (MQ=255) agaTGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTAt > 2:568509/1‑138 (MQ=255) agaTGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTAt < 1:568509/138‑1 (MQ=255) tatTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTTCGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTc > 2:791562/1‑138 (MQ=255) tatTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTc < 1:791562/138‑1 (MQ=255) tatTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCa > 1:443864/1‑140 (MQ=255) ttGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGt < 1:744059/140‑1 (MQ=255) aaGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATgg > 2:856687/1‑129 (MQ=255) aaGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATgg < 1:856687/129‑1 (MQ=255) aaGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCg > 2:831746/1‑140 (MQ=255) gcagcaACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGAt < 2:251400/140‑1 (MQ=255) gcaACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGAgggg > 1:377429/1‑140 (MQ=255) gcaACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATggg < 2:108476/140‑1 (MQ=255) gcaACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATggg > 1:108476/1‑140 (MQ=255) gcaACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATggg > 1:50723/1‑140 (MQ=255) gcaACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATggg > 1:299228/1‑140 (MQ=255) gtcgtATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGc > 1:192111/1‑140 (MQ=255) gtATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGtt < 2:42189/140‑1 (MQ=255) gtATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGtt < 2:317371/140‑1 (MQ=255) cAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCg > 2:720427/1‑140 (MQ=255) ggACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTa > 1:612245/1‑140 (MQ=255) gAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGaaaa > 2:851906/1‑140 (MQ=255) gCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCa < 1:75098/140‑1 (MQ=255) gCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCa < 1:831746/140‑1 (MQ=255) cTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAg > 1:584276/1‑140 (MQ=255) cTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAg > 1:394971/1‑140 (MQ=255) cTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAg > 1:455341/1‑140 (MQ=255) cTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAg > 1:472007/1‑140 (MQ=255) gTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAGGAAAATTCAGGATg < 2:443864/140‑1 (MQ=255) cATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCg > 2:209188/1‑140 (MQ=255) cATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCg < 2:612245/140‑1 (MQ=255) cATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCg > 1:538334/1‑140 (MQ=255) tctcCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCa < 1:851906/140‑1 (MQ=255) ctcCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAg < 2:584276/140‑1 (MQ=255) ctcCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAg < 2:472007/140‑1 (MQ=255) ccGCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGAt < 2:299228/140‑1 (MQ=255) gCTGTCTGCTGGCGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGAttt < 2:192111/140‑1 (MQ=255) cGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACa < 2:50723/140‑1 (MQ=255) cGATGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACa < 2:394971/140‑1 (MQ=255) aTGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGAttt > 1:402075/1‑126 (MQ=255) aTGTGACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGAttt < 2:402075/126‑1 (MQ=255) tgtgACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAgcg < 1:209188/140‑1 (MQ=255) gtgACTAACGGCCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAgcgc > 2:13117/1‑140 (MQ=255) gtgACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTTTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAgcgc > 1:593074/1‑140 (MQ=255) gtgACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAgcgc < 2:394177/140‑1 (MQ=255) gtgACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAgcgc < 2:187826/140‑1 (MQ=255) gtgACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAgcg < 2:502379/139‑1 (MQ=255) gtgACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAgcg > 1:502379/1‑139 (MQ=255) gtgACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAgcg < 2:806242/139‑1 (MQ=255) gtgACTAACGACCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAgcg > 1:806242/1‑139 (MQ=255) aCCTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCaacaac < 1:720427/140‑1 (MQ=255) cTGAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCt < 1:13117/140‑1 (MQ=255) gAGTCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTgc > 2:53934/1‑140 (MQ=255) | TATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGC > NC_000913/3773273‑3773541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |