Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,004,479	A→G	G73G (GGT→GGC)	yigI ←	putative thioesterase YigI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,004,479	0	A	G	100.0%	69.6 / NA	21	G73G (GGT→GGC)	yigI	putative thioesterase YigI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/12);  total (9/12)

AACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCA‑CACCAGACCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCC  >  NC_000913/4004349‑4004613                                                                                                                                    |                                                                                                                                       aaCGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCA‑CACCAGGCCGgcggcg                                                                                                                               <  1:348180/140‑1 (MQ=255)               cctgGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGa                                                                                                                 <  1:753896/140‑1 (MQ=255)                    gcgcAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGGTGAAGTACTTCCCCCGCACCCCCAGCCCCG‑GGCGGCATCCCGCCCCCACGccc                                                                                                            >  2:753896/1‑138 (MQ=255)                                          tCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCt                                                                                      <  2:527873/140‑1 (MQ=255)                                               ggTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTgcg                                                                                 <  1:147000/140‑1 (MQ=255)                                                 tCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGcc                                                                               <  1:835166/140‑1 (MQ=255)                                                     cATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGt                                                                           <  1:774086/140‑1 (MQ=255)                                                            gATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCccac                                                                    <  1:136859/140‑1 (MQ=255)                                                                               cATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGTCCACCATCATTGGCTGATTTTTa                                                 <  1:511263/140‑1 (MQ=255)                                                                                       cTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAg                                         <  1:590538/140‑1 (MQ=255)                                                                                             gTTTCGTGGCGGGTTAAGGTACTTCCCACGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGc                                   <  2:597294/140‑1 (MQ=255)                                                                                                     gCGGGTTAAGGTACTTCCCACGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCtt                           <  2:625478/140‑1 (MQ=255)                                                                                                                   ttCCCACGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTcc             >  1:721250/1‑140 (MQ=255)                                                                                                                   ttCCCACGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTcc             >  1:253943/1‑140 (MQ=255)                                                                                                                   ttCCCACGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTcc             >  1:277976/1‑140 (MQ=255)                                                                                                                   ttCCCACGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACCCTTTTTCGTAACGCTcc             >  2:862322/1‑140 (MQ=255)                                                                                                                    tCCCACGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCa            >  2:804669/1‑140 (MQ=255)                                                                                                                        aCGCA‑CCCCAGGCCGGCGGCCACATCCGGCGCCGACGCAATGCCCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTc        <  2:230181/140‑1 (MQ=255)                                                                                                                        aCGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTaa                   <  2:69669/129‑1 (MQ=255)                                                                                                                        aCGCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTaa                   >  1:69669/1‑129 (MQ=255)                                                                                                                          gCA‑CACCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCa      >  2:19002/1‑140 (MQ=255)                                                                                                                              cacCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATcc   >  1:836392/1‑140 (MQ=255)                                                                                                                               acCAGGCCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATccc  >  2:466627/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                       AACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCA‑CACCAGACCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCC  >  NC_000913/4004349‑4004613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑