Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,190,784	A→G	V362A (GTA→GCA)	thiH ←	2‑iminoacetate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,190,784	0	A	G	100.0%	194.7 / NA	55	V362A (GTA→GCA)	thiH	2‑iminoacetate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/27);  total (28/27)

AACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATACCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCGAGAAGGCGCTGACGT  >  NC_000913/4190657‑4190919                                                                                                                                |                                                                                                                                        aaCACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAAc                                                                                                                             >  1:508205/1‑140 (MQ=255)  acacAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAAcc                                                                                                                            >  2:207889/1‑140 (MQ=255)  acacAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAAcc                                                                                                                            >  2:185322/1‑140 (MQ=255)                       tGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGgcagc                                                                                                       >  2:749044/1‑140 (MQ=255)                        gCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGgcagca                                                                                                      <  1:207889/140‑1 (MQ=255)                         cGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCaa                                                                                                     >  2:539381/1‑140 (MQ=255)                              ttCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCt                                                                                                >  1:225409/1‑140 (MQ=255)                               tCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCtt                                                                                               >  1:164622/1‑140 (MQ=255)                               tCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCtt                                                                                               >  1:638782/1‑140 (MQ=255)                                cATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTc                                                                                              <  1:324833/140‑1 (MQ=255)                                cATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTc                                                                                              <  2:346353/140‑1 (MQ=255)                                cATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTc                                                                                              <  1:234844/140‑1 (MQ=255)                                   cTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCgg                                                                                           <  2:140167/140‑1 (MQ=255)                                        gTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGc                                                                                      >  2:701614/1‑140 (MQ=255)                                        gTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGc                                                                                      <  2:336035/140‑1 (MQ=255)                                            cGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGAtc                                                                                  >  2:396342/1‑140 (MQ=255)                                               cTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGAtcgtc                                                                               <  2:828295/140‑1 (MQ=255)                                               cTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGAtcgtc                                                                               >  2:162316/1‑140 (MQ=255)                                                 cGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATcgtcgt                                                                             >  2:64341/1‑140 (MQ=255)                                                 cGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATcgtcgt                                                                             <  2:225409/140‑1 (MQ=255)                                                        aaTTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGCCTGCGATCGTCGTGCGGTga                                                                      >  1:488689/1‑140 (MQ=255)                                                          tttACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTgaga                                                                    <  1:185322/140‑1 (MQ=255)                                                           ttACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGaa                                                                   <  1:604175/140‑1 (MQ=255)                                                           ttACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGaa                                                                   <  1:162316/140‑1 (MQ=255)                                                           ttACGCTCTTACATTCCGTCTCATAGCCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGaa                                                                   <  2:762344/140‑1 (MQ=255)                                                            tACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAAc                                                                  <  1:519942/140‑1 (MQ=255)                                                              cGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAAc                                                                  >  2:847909/1‑138 (MQ=255)                                                              cGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAAc                                                                  <  1:847909/138‑1 (MQ=255)                                                               gTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGCCTGCGATCGTCGTGCGGTGAGAACTGt                                                               <  2:488689/140‑1 (MQ=255)                                                                           cGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCgggg                                                   <  2:508205/140‑1 (MQ=255)                                                                             tctcATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGaa                                                                   <  1:690594/122‑1 (MQ=255)                                                                             tctcATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGaa                                                                   >  2:690594/1‑122 (MQ=255)                                                                             tctcATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACt                                                        <  2:834439/133‑1 (MQ=255)                                                                             tctcATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACt                                                        >  1:834439/1‑133 (MQ=255)                                                                             tctcATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGAGGTGAGAACTGTTCCAACTCGGGGTg                                                 >  1:602398/1‑140 (MQ=255)                                                                                  tAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTAt                                            >  2:289545/1‑140 (MQ=255)                                                                                     tCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCgg                                         >  1:98083/1‑140 (MQ=255)                                                                                       tttGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCa                                       <  1:289545/140‑1 (MQ=255)                                                                                       tttGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCa                                       >  2:501518/1‑140 (MQ=255)                                                                                               gcgcGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCGAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCAcc                               >  1:720767/1‑140 (MQ=255)                                                                                               gcgcGTCCCAGATAGCTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCAcc                               >  2:39886/1‑140 (MQ=255)                                                                                                              cTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCga                >  2:261578/1‑140 (MQ=255)                                                                                                              cTGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCga                >  2:752871/1‑140 (MQ=255)                                                                                                               tGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCgag               >  2:221751/1‑140 (MQ=255)                                                                                                               tGTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCgag               <  2:762060/140‑1 (MQ=255)                                                                                                                gTCCCAGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCgaga              <  1:39886/140‑1 (MQ=255)                                                                                                                     aGTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCGAGAAGgcg         >  2:797596/1‑140 (MQ=255)                                                                                                                      gTCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTCCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCGAGAAGgcgc        >  2:221441/1‑140 (MQ=255)                                                                                                                       tCTTTCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCGAGAAGGCGCt       <  1:251741/140‑1 (MQ=255)                                                                                                                           tCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCGAGAAGGCGCTg      >  2:498244/1‑137 (MQ=255)                                                                                                                           tCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCGAGAAGGCGCTg      <  1:498244/137‑1 (MQ=255)                                                                                                                           tCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCGAGAAGGCGCTGACg   <  1:501518/140‑1 (MQ=255)                                                                                                                           tCCATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCGAGAAGGCGCTGACg   >  2:842200/1‑140 (MQ=255)                                                                                                                            ccATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCGAGAAGGCGCTGACGt  <  1:656251/140‑1 (MQ=255)                                                                                                                            ccATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCGAGAAGGCGCTGACGt  <  1:749044/140‑1 (MQ=255)                                                                                                                             cATGCCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCgaga              <  1:648931/127‑1 (MQ=255)                                                                                                                                |                                                                                                                                        AACACAACGCTTTCAACAACCTTGCGTTTTTCATCTTCCGTAGCGGCTCGGAATAAATTTACGTTCTTACATTCCGTCTCATAGTCTTTGCGAGGCGCGTCCCAGATAGCTGTCCCAGTCTTTCCATACCGGCTGCAAACCCTGAGCGGTTAACGCGGCAGCAACCGCTTCCGGTCTGCGATCGTCGTGCGGTGAGAACTGTTCCAACTCGGGGTGATTATCGGCATAGCCACCTGGCTGCGTTTTCGAGAAGGCGCTGACGT  >  NC_000913/4190657‑4190919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑