Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,467,200	A→G	V16A (GTG→GCG)	treR ←	DNA‑binding transcriptional repressor TreR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,467,200	0	A	G	100.0%	193.8 / NA	54	V16A (GTG→GCG)	treR	DNA‑binding transcriptional repressor TreR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/26);  total (28/26)

TTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTT  >  NC_000913/4467067‑4467335                                                                                                                                       |                                                                                                                                        ttGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGcgccgc                                                                                                                                   >  1:470181/1‑140 (MQ=255)  tGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCt                                                                                                                                  >  1:484122/1‑140 (MQ=255)   gCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCtt                                                                                                                                 >  2:423349/1‑140 (MQ=255)        cGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAgcg                                                                                                                            <  1:423349/140‑1 (MQ=255)         gCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAgcgc                                                                                                                           <  2:859227/140‑1 (MQ=255)          cATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAgcgcg                                                                                                                          <  2:595683/140‑1 (MQ=255)          cATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAgcgcg                                                                                                                          <  1:590660/140‑1 (MQ=255)                   cgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCtt                                                                                                                 >  1:132950/1‑140 (MQ=255)                   cgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCtt                                                                                                                 >  1:297312/1‑140 (MQ=255)                   cgcAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCtt                                                                                                                 >  1:174022/1‑140 (MQ=255)                         gCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATgg                                                                                                           >  1:808884/1‑140 (MQ=255)                                  ggAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGAt                                                                                                  >  2:434540/1‑140 (MQ=255)                                  ggAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGAt                                                                                                  >  1:567266/1‑140 (MQ=255)                                   gAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGAtt                                                                                                 <  2:132950/140‑1 (MQ=255)                                    aaaaTCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGATATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGAttt                                                                                                >  1:752091/1‑140 (MQ=255)                                       aTCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGc                                                                                             >  2:527524/1‑140 (MQ=255)                                        tCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCa                                                                                            <  1:305705/140‑1 (MQ=255)                                        tCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCa                                                                                            <  1:67306/140‑1 (MQ=255)                                        tCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCa                                                                                            <  1:519481/140‑1 (MQ=255)                                           aTGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATcc                                                                                         <  2:38368/140‑1 (MQ=255)                                             gCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCtg                                                                                       <  1:159473/140‑1 (MQ=255)                                                 aTTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCtgttgt                                                                                   >  1:414146/1‑140 (MQ=255)                                                 aTTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCtgttgt                                                                                   >  1:326937/1‑140 (MQ=255)                                                  ttCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTc                                                                                  <  1:434540/140‑1 (MQ=255)                                                      tcaCTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCTCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGt                                                                              >  1:625170/1‑140 (MQ=255)                                                        aCTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTaa                                                                            >  1:384259/1‑140 (MQ=255)                                                           gCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACgt                                                                         >  2:12524/1‑140 (MQ=255)                                                            cTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACgtg                                                                        <  2:470181/140‑1 (MQ=255)                                                                 acacGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTgttgtt                                                                   >  1:196575/1‑140 (MQ=255)                                                                   acGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTa                                                                 <  2:484122/140‑1 (MQ=255)                                                                     gCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAat                                                               >  2:558312/1‑140 (MQ=255)                                                                          cgGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTAttt                                                          <  2:343199/140‑1 (MQ=255)                                                                             gTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAg                                                       <  2:567266/140‑1 (MQ=255)                                                                              tGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGc                                                      <  2:307644/140‑1 (MQ=255)                                                                               gAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGcc                                                     >  1:353638/1‑140 (MQ=255)                                                                                aGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCt                                                    >  1:104712/1‑140 (MQ=255)                                                                                 gCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTa                                                   >  2:725584/1‑140 (MQ=255)                                                                                    ggCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACg                                                <  2:711550/140‑1 (MQ=255)                                                                                     gCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGt                                               <  1:527524/140‑1 (MQ=255)                                                                                            ccgcTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGt                                        <  2:326937/140‑1 (MQ=255)                                                                                             cccTTTCGTTATTCAACACCCGGGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGt                                        <  2:262313/138‑1 (MQ=255)                                                                                               cTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCa                                     >  2:201255/1‑140 (MQ=255)                                                                                               cTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCa                                     >  1:743125/1‑140 (MQ=255)                                                                                                 ttCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCAtt                                   <  2:353638/140‑1 (MQ=255)                                                                                                     ttATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGc                               >  2:229860/1‑140 (MQ=255)                                                                                                      tATTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCa                              <  2:752091/140‑1 (MQ=255)                                                                                                       aTTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCaa                             <  2:104712/140‑1 (MQ=255)                                                                                                       aTTCAGCACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCaa                             <  2:174022/140‑1 (MQ=255)                                                                                                             cACCC‑GGGAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCAATGGGTa                       >  1:403822/1‑140 (MQ=255)                                                                                                                   gggAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCAAt                            <  1:99269/130‑1 (MQ=255)                                                                                                                   gggAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCAAt                            >  2:99269/1‑130 (MQ=255)                                                                                                                   gggAAACTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCAATGGGTAAAGTc                  >  1:254852/1‑140 (MQ=255)                                                                                                                        aCTGTAGATTTCCCCGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCAATGGGTAAAGTCTGGtt             >  2:615183/1‑140 (MQ=255)                                                                                                                                   ccccGCGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGtt  <  2:403822/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TTGCCCACGCATAGCGCGCGCAGAGCGGGAAGGGGAAAATCCATGCTGATTCATCACTGCTTCAACACGCTCGCGGGTGAGCTGGCTCACGCCGCTTTCGTTATTCAGCACCC‑GGGAAACTGTAGATTTCCCCACGCCGCTTAAGCGCGCGATATCTTTGATGGTCAGCCGATTTTGCATCCTGTTGTCCTGTAACGTGTTGTTTAATTATTTGAGCCTAACGTTACCCGTGCATTCAGCAATGGGTAAAGTCTGGTTTATCGTTGGTT  >  NC_000913/4467067‑4467335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑