Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,542,303	A→G	E89E (GAA→GAG)	fimE →	regulator for fimA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,542,303	0	A	G	100.0%	282.9 / NA	78	E89E (GAA→GAG)	fimE	regulator for fimA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/39);  total (39/39)

CTGCTTGATCTGCATTATCAGGACCTTGACCTTAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTC  >  NC_000913/4542169‑4542436                                                                                                                                       |                                                                                                                                      cTGCTTGATCTGCATTATCAGGACCTTGACCTTAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGc                                                                                                                                  >  2:305751/1‑140 (MQ=255) cTGCTTGATCTGCATTATCAGGACCTTGACCTTAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGc                                                                                                                                  >  2:326775/1‑140 (MQ=255) cTGCTTGATCTGCATTATCAGGACCTTGACCTTAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGc                                                                                                                                  >  2:424423/1‑140 (MQ=255)    cTTGATCTGCATTATCAGGACCTTGACCTTAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTaa                                                                                                                               >  1:61650/1‑140 (MQ=255)    cTTGATCTGCATTATCAGGACCTTGACCTTAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTaa                                                                                                                               >  2:273526/1‑140 (MQ=255)     ttGATCTGCATTATCAGGACCTTGACCTTAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAAc                                                                                                                              <  1:836080/140‑1 (MQ=255)               ttATCAGGACCTTGACCTTAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGgcg                                                                                                                    <  1:189770/140‑1 (MQ=255)                  tCAGGACCTTGACCTTAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTg                                                                                                                 >  2:303850/1‑140 (MQ=255)                  tCAGGACCTTGACCTTAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTg                                                                                                                 >  2:452977/1‑140 (MQ=255)                       accttgaccttAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCgg                                                                                                            >  2:73193/1‑140 (MQ=255)                        ccttgaccttAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCCACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGa                                                                                                           <  2:121579/140‑1 (MQ=255)                         cttgaccttAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGAc                                                                                                          <  1:139547/140‑1 (MQ=255)                         cttgaccttAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGAc                                                                                                          >  2:249303/1‑140 (MQ=255)                            gaccttAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGa                                                                                                       >  1:41908/1‑140 (MQ=255)                            gaccttAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGa                                                                                                       <  1:424423/140‑1 (MQ=255)                            gaccttAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGa                                                                                                       >  2:68916/1‑140 (MQ=255)                            gaccttAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGa                                                                                                       >  1:518926/1‑140 (MQ=255)                            gaccttAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGa                                                                                                       >  1:193675/1‑140 (MQ=255)                            gaccttAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGa                                                                                                       >  2:139468/1‑140 (MQ=255)                            gaccttAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGa                                                                                                       >  1:570588/1‑140 (MQ=255)                              ccttAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACg                                                                                                     <  1:73193/140‑1 (MQ=255)                               cttAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGc                                                                                                    <  1:23726/140‑1 (MQ=255)                                ttAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCt                                                                                                   <  2:813479/140‑1 (MQ=255)                                  aaTGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCtat                                                                                                 >  1:428805/1‑140 (MQ=255)                                  aaTGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCtat                                                                                                 >  2:439298/1‑140 (MQ=255)                                  aaTGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCtat                                                                                                 >  1:30469/1‑140 (MQ=255)                                   aTGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCtata                                                                                                >  1:784022/1‑140 (MQ=255)                                            gAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACt                                                                                                         >  1:538821/1‑122 (MQ=255)                                            gAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACt                                                                                                         <  2:538821/122‑1 (MQ=255)                                            gAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTtct                                                                                       <  1:841218/140‑1 (MQ=255)                                            gAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTtct                                                                                       <  1:637165/140‑1 (MQ=255)                                            gAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTtct                                                                                       >  2:193935/1‑140 (MQ=255)                                             aataaataTTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTtctc                                                                                      >  1:89269/1‑140 (MQ=255)                                              ataaataTTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTcg                                                                                     <  2:837695/140‑1 (MQ=255)                                                  atatTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACACTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTcgccgc                                                                                 >  2:481444/1‑140 (MQ=255)                                                   tatTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCcgcg                                                                                >  1:727718/1‑140 (MQ=255)                                                       cGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAg                                                                            >  2:758295/1‑140 (MQ=255)                                                       cGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAg                                                                            >  2:639945/1‑140 (MQ=255)                                                       cGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAg                                                                            >  1:242756/1‑140 (MQ=255)                                                        gCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGt                                                                           <  1:139468/140‑1 (MQ=255)                                                         ccGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTc                                                                          >  1:283746/1‑140 (MQ=255)                                                             cTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCt                                                                      <  2:784022/140‑1 (MQ=255)                                                             cTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCt                                                                      <  1:439298/140‑1 (MQ=255)                                                                aagaaCGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTtc                                                                   >  2:321385/1‑140 (MQ=255)                                                                       gATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCcag                                                            <  1:512176/140‑1 (MQ=255)                                                                        aTTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATGTTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCcagc                                                           >  2:592668/1‑140 (MQ=255)                                                                        aTTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACACAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTtct                                                                  <  2:826457/133‑1 (MQ=255)                                                                        aTTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACACAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTtct                                                                  >  1:826457/1‑133 (MQ=255)                                                                                  gTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACTGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCg                                                 >  1:432356/1‑140 (MQ=255)                                                                                  gTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATGTTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCg                                                 <  1:592668/140‑1 (MQ=255)                                                                                  gTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCg                                                 >  1:364222/1‑140 (MQ=255)                                                                                   ttCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGc                                                >  2:557522/1‑140 (MQ=255)                                                                                    tCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCa                                               <  2:428805/140‑1 (MQ=255)                                                                                          gTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTc                                         >  1:624080/1‑140 (MQ=255)                                                                                           ttACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTcg                                        <  2:283746/140‑1 (MQ=255)                                                                                             aCGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTcgcg                                      <  1:794800/140‑1 (MQ=255)                                                                                              cGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCGGCAGGCCTATCGCATTATTCGCGa                                     <  1:464222/140‑1 (MQ=255)                                                                                                 tttGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGc                                  <  1:321385/140‑1 (MQ=255)                                                                                                 tttGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGc                                  <  2:518926/140‑1 (MQ=255)                                                                                                 tttGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGc                                  <  1:826495/140‑1 (MQ=255)                                                                                                 tttGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGc                                  <  2:624080/140‑1 (MQ=255)                                                                                                 tttGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGc                                  <  1:4675/140‑1 (MQ=255)                                                                                                 tttGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGc                                  <  2:695360/140‑1 (MQ=255)                                                                                                        aGCGTGAAGCCGTGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTAtt                           <  2:364222/140‑1 (MQ=255)                                                                                                                    tGGAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAAcc               <  1:273526/140‑1 (MQ=255)                                                                                                                     ggAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCg              <  2:41908/140‑1 (MQ=255)                                                                                                                     ggAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCg              <  1:557522/140‑1 (MQ=255)                                                                                                                      gAACGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGt             >  2:756881/1‑140 (MQ=255)                                                                                                                         cGCTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAAc          >  1:774675/1‑140 (MQ=255)                                                                                                                           cTGGACCTAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCg              <  1:476792/134‑1 (MQ=255)                                                                                                                           cTGGACCTAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCg              >  2:476792/1‑134 (MQ=255)                                                                                                                           cTGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGc        >  2:795821/1‑140 (MQ=255)                                                                                                                            tGGACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCa       <  2:89269/140‑1 (MQ=255)                                                                                                                              gACCCAGGAGCGTGCTAACTGGAAAGGCGCTGACTGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGa     <  2:432356/140‑1 (MQ=255)                                                                                                                               aCCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGAc    <  1:452977/140‑1 (MQ=255)                                                                                                                               aCCCAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGAc    <  1:305751/140‑1 (MQ=255)                                                                                                                                cccAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACt   <  2:193675/140‑1 (MQ=255)                                                                                                                                 ccAGGAGCGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTc  <  2:242756/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      CTGCTTGATCTGCATTATCAGGACCTTGACCTTAATGAAGGTAGAATAAATATTCGCCGACTGAAGAACGGATTTTCTACCGTTCACCCGTTACGTTTTGATGAGCGTGAAGCCGTGGAACGCTGGACCCAGGAACGTGCTAACTGGAAAGGCGCTGACCGGACTGACGCTATATTTATTTCTCGCCGCGGGAGTCGGCTTTCTCGCCAGCAGGCCTATCGCATTATTCGCGATGCCGGTATTGAAGCTGGAACCGTAACGCAGACTC  >  NC_000913/4542169‑4542436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑