Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,565,114	C→T	G246D (GGC→GAC)	yjiM ←	putative dehydratase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,565,114	0	C	T	100.0%	170.8 / NA	50	G246D (GGC→GAC)	yjiM	putative dehydratase subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/28);  total (22/28)

GTCGTAGACATCGCCCGTTTCTGCCACGCATTGCTCGGTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCA  >  NC_000913/4564978‑4565246                                                                                                                                         |                                                                                                                                     gtcgtAGACATCGCCCGTTTCTGCCACGCATTGCTCGGTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCgg                                                                                                                                   <  2:382875/140‑1 (MQ=255)        aCATCGCCCGTTTCTGCCACGCATTGCTCGGTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTaa                                                                                                                            >  1:548491/1‑140 (MQ=255)            cGCCCGTTTCTGCCACGCATTGCTCGGTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATg                                                                                                                        >  1:449854/1‑140 (MQ=255)            cGCCCGTTTCTGCCACGCATTGCTCGGTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATg                                                                                                                        >  1:640392/1‑140 (MQ=255)            cGCCCGTTTCTGCCACGCATTGCTCGGTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGAAGTCGGTGATTAAAATg                                                                                                                        >  2:628762/1‑140 (MQ=255)             gCCCGTTTCTGCCACGCATTGCTCGGTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATgc                                                                                                                       >  2:338676/1‑140 (MQ=255)              cccGTTTCTGCCACGCATTGCTCGGTCGCTTTCGCCCCGGTGCAGTTTACATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATgcg                                                                                                                      >  2:665460/1‑140 (MQ=255)               ccGTTTCTGCCACGCATTGCTCGGTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATgcgc                                                                                                                     >  2:739325/1‑140 (MQ=255)                   ttCTGCCACGCATTGCTCGGTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGAc                                                                                                                 >  2:784694/1‑140 (MQ=255)                                     ggTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGc                                                                                               <  2:449854/140‑1 (MQ=255)                                     ggTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGc                                                                                               <  1:739325/140‑1 (MQ=255)                                       tCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCACCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCct                                                                                             >  2:159035/1‑140 (MQ=255)                                             tCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTcc                                                                                       <  1:338676/140‑1 (MQ=255)                                               gCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCa                                                                                     <  2:135032/140‑1 (MQ=255)                                               gCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCa                                                                                     <  2:590262/140‑1 (MQ=255)                                                 cccGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCAct                                                                                   >  1:636896/1‑140 (MQ=255)                                                 cccGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCAct                                                                                   >  2:746763/1‑140 (MQ=255)                                                     gTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGc                                                                                               >  1:656134/1‑124 (MQ=255)                                                     gTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGc                                                                                               <  2:656134/124‑1 (MQ=255)                                                      tGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGa                                                                              <  2:791148/140‑1 (MQ=255)                                                              tCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAAcgcg                                                                      <  2:548491/140‑1 (MQ=255)                                                                aTAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACgcggg                                                                    <  2:861569/140‑1 (MQ=255)                                                                aTAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGAAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACgcggg                                                                    <  1:628762/140‑1 (MQ=255)                                                                 tAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACgcgggc                                                                   <  2:291032/140‑1 (MQ=255)                                                                     cGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTc                                                               >  1:821682/1‑140 (MQ=255)                                                                     cGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTc                                                               <  1:307567/140‑1 (MQ=255)                                                                       aCAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCAt                                                             >  1:761895/1‑140 (MQ=255)                                                                         aaCCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTg                                                           >  2:462713/1‑140 (MQ=255)                                                                             cAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATc                                                       <  1:159035/140‑1 (MQ=255)                                                                             cAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATc                                                       <  2:732980/140‑1 (MQ=255)                                                                             cAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATc                                                       <  1:665460/140‑1 (MQ=255)                                                                              aGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATcc                                                      >  1:480567/1‑140 (MQ=255)                                                                                    cATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCa                                                <  1:462713/140‑1 (MQ=255)                                                                                    cATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCa                                                <  2:636896/140‑1 (MQ=255)                                                                                         tctTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGAt                                           <  2:640392/140‑1 (MQ=255)                                                                                            tCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCaa                                        <  1:784694/140‑1 (MQ=255)                                                                                                   cgcgcACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCctct                                 >  2:831639/1‑140 (MQ=255)                                                                                                     cgcACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCttt                               >  2:744654/1‑140 (MQ=255)                                                                                                      gcACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTa                              <  2:480567/140‑1 (MQ=255)                                                                                                       caccacTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGCTTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTTATTGCATCCAGTTCATTGATCAACGCCTCTTTAt                             <  2:761895/140‑1 (MQ=255)                                                                                                         ccacTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCCGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCg                           <  1:746763/140‑1 (MQ=255)                                                                                                            cTTTTTCTGCTGCGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAAc                        >  2:623300/1‑140 (MQ=255)                                                                                                                    gctgcGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGt                >  2:381410/1‑140 (MQ=255)                                                                                                                     ctgcGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGtt               <  1:744654/140‑1 (MQ=255)                                                                                                                      tgcGCCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTg              >  1:859617/1‑140 (MQ=255)                                                                                                                       gcgcCGCCAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTgc             >  2:73462/1‑140 (MQ=255)                                                                                                                            gccAATCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGt        <  2:187184/140‑1 (MQ=255)                                                                                                                                aTCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAAc    <  2:859617/140‑1 (MQ=255)                                                                                                                                aTCGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAAc    <  1:125792/140‑1 (MQ=255)                                                                                                                                  cGGGCAGTCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCa  <  1:73462/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     GTCGTAGACATCGCCCGTTTCTGCCACGCATTGCTCGGTCGCTTTCGCCCCGGTGCAGTTTTCATAACCGACAACCCAGCCGCCATTCTCTTCAATCGCGCGCACCACTTTTTCTGCTGCGCCGCCAATCGGGCAGCCGGTGATTAAAATGCGCGGACGCGGGTCCAGTCGCTGGCCTTCTTCCCACTGCTGACGAACGCGGGCGGTCATTGCATCCAGTTCATTGATCAACGCCTCTTTATCGAACCGGAAGGTTGCGCCGTAAACCA  >  NC_000913/4564978‑4565246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑