Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,093,886	Δ1 bp	coding (419/1068 nt)	hisB →	imidazoleglycerol‑phosphate dehydratase/histidinol‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,093,883	0	A	.	100.0%	187.6 / NA	45	coding (416/1068 nt)	hisB	imidazoleglycerol‑phosphate dehydratase/histidinol‑phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (17/28);  total (17/28)

TGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAG  >  NC_000913/2093744‑2094016                                                                                                                                            |                                                                                                                                      tgcccGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAGACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGa                                                                                                                                       <  2:703884/137‑1 (MQ=255)   tCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGaaa                                                                                                                                     >  2:408190/1‑140 (MQ=255)    ccGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGaaac                                                                                                                                    <  2:659695/140‑2 (MQ=255)     cGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGaaaca                                                                                                                                   >  1:647497/1‑138 (MQ=255)         ccTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATggg                                                                                                                              <  1:323077/140‑1 (MQ=255)         ccTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATggg                                                                                                                              <  2:113312/140‑1 (MQ=255)         ccTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATggg                                                                                                                              <  1:662308/140‑1 (MQ=255)         ccTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATggg                                                                                                                              <  1:639210/140‑1 (MQ=255)                                 ccGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACcg                                                                                                      >  1:650870/1‑140 (MQ=255)                                 ccGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACcg                                                                                                      >  1:640003/1‑140 (MQ=255)                                   gTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACcgcg                                                                                                    <  1:118720/140‑1 (MQ=255)                                   gTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACcgcg                                                                                                    <  1:339981/140‑1 (MQ=255)                                            aaGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGa                                                                                           >  2:694659/1‑140 (MQ=255)                                            aaGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGa                                                                                           <  1:443030/140‑1 (MQ=255)                                             aGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGaa                                                                                          <  1:694659/140‑1 (MQ=255)                                             aGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGaa                                                                                          <  2:208245/140‑1 (MQ=255)                                                                 aTCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGCCCGCGAAACCCTGAACTGGCCAATGATTGGCGAGc                                                                      <  2:74185/140‑1 (MQ=255)                                                                 aTCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGc                                                                      <  1:408190/140‑1 (MQ=255)                                                                   cTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCaa                                                                    <  2:647497/140‑1 (MQ=255)                                                                       cTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCa                                                                <  1:315879/140‑1 (MQ=255)                                                                         gAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCAcc                                                              >  2:409060/1‑140 (MQ=255)                                                                          aGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCa                                                             >  2:650010/1‑140 (MQ=255)                                                                          aGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCa                                                             >  2:531841/1‑140 (MQ=255)                                                                            cAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGa                                                           <  1:501966/140‑1 (MQ=255)                                                                            cAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGa                                                           <  2:314036/140‑1 (MQ=255)                                                                            cAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGa                                                           <  1:720148/140‑1 (MQ=255)                                                                                cGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTg                                                       >  2:366872/1‑140 (MQ=255)                                                                                         gcgcTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACg                                              <  2:650870/140‑1 (MQ=255)                                                                                         gcgcTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACg                                              <  2:640003/140‑1 (MQ=255)                                                                                         gcgcTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACg                                              <  1:650010/140‑1 (MQ=255)                                                                                         gcgcTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACg                                              <  1:214020/140‑1 (MQ=255)                                                                                          cgcTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGc                                             >  2:291889/1‑140 (MQ=255)                                                                                          cgcTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGc                                             >  1:10914/1‑140 (MQ=255)                                                                                          cgcTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGc                                             >  1:20625/1‑140 (MQ=255)                                                                                            cTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTc                                           <  2:132475/140‑1 (MQ=255)                                                                                            cTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTc                                           <  1:500399/140‑1 (MQ=255)                                                                                               aCAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACg                                        <  1:84880/140‑1 (MQ=255)                                                                                               aCAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACg                                        <  1:291889/140‑1 (MQ=255)                                                                                               aCAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACg                                        <  1:366872/140‑1 (MQ=255)                                                                                                    tATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTg                                   >  2:545244/1‑140 (MQ=255)                                                                                                     aTGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGc                                  >  2:607620/1‑140 (MQ=255)                                                                                                       gtgATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt                                >  1:598701/1‑140 (MQ=255)                                                                                                        tgATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTa                               <  1:409060/140‑1 (MQ=255)                                                                                                         gATTGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa                              <  1:531841/140‑1 (MQ=255)                                                                                                           ttGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATa                            >  2:611481/1‑140 (MQ=255)                                                                                                           ttGGCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATa                            >  1:359551/1‑140 (MQ=255)                                                                                                             ggCGATCGCGCGACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATAcc                          >  1:550575/1‑140 (MQ=255)                                                                                                                    gcgcgACCGACATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAgaga                   <  2:459312/140‑1 (MQ=255)                                                                                                                           cgacATTCAACTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAt            <  2:550575/140‑1 (MQ=255)                                                                                                                                     cTGGCGG‑AAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAg  >  1:361109/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                      TGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAG  >  NC_000913/2093744‑2094016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑