Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,127,329	(T)5→4	coding (986/1122 nt)	gmd ←	GDP‑mannose 4,6‑dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,127,325	0	T	.	100.0%	174.6 / NA	42	coding (990/1122 nt)	gmd	GDP‑mannose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (26/16);  total (26/16)

TGCTTATGACTCCAGCGCGATCGCCACGTCGTAGCCGTGAGATTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGAAACCACAATG  >  NC_000913/2127190‑2127460                                                                                                                                        |                                                                                                                                        tGCTTATGACTCCAGCGCGATCGCCACGTCGTAGCCGTGAGATTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGttttc                                                                                                                                     >  1:271301/1‑139 (MQ=255)   cTTATGACTCCAGCGCGATCGCCACGTCGTAGCCGTGAGATTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGttttcgt                                                                                                                                   >  1:423957/1‑137 (MQ=255)      aTGACTCCAGCGCGATCGCCACGTCGTAGCCGTGAGATTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTgcg                                                                                                                               <  2:580684/140‑1 (MQ=255)       tGACTCCAGCGCGATCGCCACGTCGTAGCCGTGAGATTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTgcgc                                                                                                                              >  1:618116/1‑140 (MQ=255)       tGACTCCAGCGCGATCGCCACGTCGTAGCCGTGAGATTTCAGCAGAGAGAGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTgcgc                                                                                                                              >  2:285070/1‑140 (MQ=255)        gACTCCAGCGCGATCGCCACGTCGTAGCCGTGAGATTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCt                                                                                                                             >  1:191288/1‑140 (MQ=255)                     tCGCCACGTCGTAGCCGTGAGATTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGc                                                                                                                >  2:229867/1‑140 (MQ=255)                                 aGCCGTGAGATTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGagc                                                                                                           >  2:172689/1‑133 (MQ=255)                                 aGCCGTGAGATTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGagc                                                                                                           <  1:172689/133‑1 (MQ=255)                                   ccGTGAGATTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCa                                                                                                  >  2:697572/1‑140 (MQ=255)                                        agaTTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTc                                                                                             >  1:103649/1‑140 (MQ=255)                                               agcagAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCcgga                                                                                      <  2:618116/140‑1 (MQ=255)                                                      agTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCcgg                                                                                       <  1:206902/132‑1 (MQ=255)                                                      agTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCcgg                                                                                       >  2:206902/1‑132 (MQ=255)                                                          ttttttCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAAcg                                                                           <  2:191288/140‑1 (MQ=255)                                                                  ccgcTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCaa                                                                   >  2:601964/1‑140 (MQ=255)                                                                     cTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAg                                                                <  2:103649/140‑1 (MQ=255)                                                                                   gCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAatcacatca                                                  <  1:285070/140‑1 (MQ=255)                                                                                     caccaTTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCAcc                                                >  1:136895/1‑140 (MQ=255)                                                                                       ccaTTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCg                                              >  2:702107/1‑140 (MQ=255)                                                                                        caTTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCgg                                             >  2:538458/1‑140 (MQ=255)                                                                                           ttCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGttt                                          >  2:511368/1‑140 (MQ=255)                                                                                             cAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTaa                                        >  2:169419/1‑140 (MQ=255)                                                                                                     aTCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGgc                                >  1:390109/1‑140 (MQ=255)                                                                                                     aTCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGgc                                <  1:601964/140‑1 (MQ=255)                                                                                                     aTCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGgc                                >  2:108144/1‑140 (MQ=255)                                                                                                         ctctGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGt                            >  1:500759/1‑140 (MQ=255)                                                                                                          tctGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTc                           <  1:538458/140‑1 (MQ=255)                                                                                                            tGAGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGACGCGTCAt                         >  2:590645/1‑140 (MQ=255)                                                                                                              aGGGTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGc                       >  2:355494/1‑140 (MQ=255)                                                                                                               gggTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGcc                      <  1:412490/140‑1 (MQ=255)                                                                                                                ggTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGccc                     >  1:317160/1‑140 (MQ=255)                                                                                                                ggTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGccc                     >  1:127892/1‑140 (MQ=255)                                                                                                                 gTGATTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCg                    >  1:329414/1‑140 (MQ=255)                                                                                                                    aTTTCCGGTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTg                 <  1:229867/140‑1 (MQ=255)                                                                                                                          ggTTTCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGaa           >  2:332654/1‑140 (MQ=255)                                                                                                                              tCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGAAACca       >  2:81313/1‑140 (MQ=255)                                                                                                                              tCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGAAACca       <  1:702107/140‑1 (MQ=255)                                                                                                                              tCCAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGAAACca       <  1:522167/140‑1 (MQ=255)                                                                                                                                cAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGAAACcaca     <  2:500759/140‑1 (MQ=255)                                                                                                                                cAGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGAAACcaca     <  1:355494/140‑1 (MQ=255)                                                                                                                                 aGCCCAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGCTTAACGCCCGGCGCGTCATGCCCGGTGACGGAAACCACaa    >  2:601649/1‑140 (MQ=255)                                                                                                                                   cccAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGAAACCACAATg  <  1:81313/140‑1 (MQ=255)                                                                                                                                   cccAG‑TTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGAAACCACAATg  >  1:376798/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TGCTTATGACTCCAGCGCGATCGCCACGTCGTAGCCGTGAGATTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGAAACCACAATG  >  NC_000913/2127190‑2127460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑