Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,383,720	(C)5→4	coding (1008/1212 nt)	elaD →	protease ElaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,383,716	0	C	.	100.0%	162.1 / NA	39	coding (1004/1212 nt)	elaD	protease ElaD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (22/17);  total (22/17)

ATTTAAATGAACCAGAGATAATATTTTTACATGCCGATCTTCAGCAATACTTAAGCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCGCCCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCA  >  NC_000913/2383587‑2383850                                                                                                                                  |                                                                                                                                       aTTTAAATGAACCAGAGATAATATTTTTACATGCCGATCTTCAGCAATACTTAAGCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGt                                                                                                                             <  2:84156/140‑1 (MQ=255) aTTTAAATGAACCAGAGATAATATTTTTACATGCCGATCTTCAGCAATACTTAAGCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGt                                                                                                                             <  2:452678/140‑1 (MQ=255)           aCCAGAGATAATATTTTTACATGCCGATCTTCAGCAATACTTAAGCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGt                                                                                                                             <  1:41639/130‑1 (MQ=255)           aCCAGAGATAATATTTTTACATGCCGATCTTCAGCAATACTTAAGCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGt                                                                                                                             >  2:41639/1‑130 (MQ=255)                agaTAATATTTTTACATGCCGATCTTCAGCAATACTTAAGCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATg                                                                                                              >  2:555858/1‑140 (MQ=255)                     atatTTTTACATGCCGATCTTCAGCAATACTTAAGCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGAc                                                                                                         <  2:151519/140‑1 (MQ=255)                                tGCCGATCTTCAGCAATACTTAAGCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGa                                                                                                          >  1:650346/1‑128 (MQ=255)                                tGCCGATCTTCAGCAATACTTAAGCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGCTATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGa                                                                                                          <  2:650346/128‑1 (MQ=255)                                                tACTTAAGCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAagag                                                                              >  2:331886/1‑140 (MQ=255)                                                tACTTAAGCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAagag                                                                              >  2:14874/1‑140 (MQ=255)                                                     aaGCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCagaag                                                                                >  1:143995/1‑133 (MQ=255)                                                     aaGCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCagaag                                                                                <  2:143995/133‑1 (MQ=255)                                                       gCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACg                                                                       >  2:677341/1‑140 (MQ=255)                                                       gCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACg                                                                       >  2:558415/1‑140 (MQ=255)                                                            aGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAAtt                                                                  >  2:261224/1‑140 (MQ=255)                                                            aGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAAtt                                                                  >  2:53693/1‑140 (MQ=255)                                                                      cATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAAc                                                        >  2:597348/1‑140 (MQ=255)                                                                      cATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAAc                                                        >  2:567370/1‑140 (MQ=255)                                                                              tgCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTg                                                >  1:56739/1‑140 (MQ=255)                                                                                 aTGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCa                                             <  1:53693/140‑1 (MQ=255)                                                                                   ggCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCaaa                                           >  2:224786/1‑140 (MQ=255)                                                                                     cAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACa                                         >  2:700334/1‑140 (MQ=255)                                                                                      aGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAg                                        >  1:441725/1‑140 (MQ=255)                                                                                       gCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGa                                       <  2:436036/140‑1 (MQ=255)                                                                                       gCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGa                                       <  1:597348/140‑1 (MQ=255)                                                                                        cccAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGaa                                      >  2:407145/1‑140 (MQ=255)                                                                                         ccAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGaaa                                     <  2:426291/140‑1 (MQ=255)                                                                                           aGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGAAAtt                                   <  1:677341/140‑1 (MQ=255)                                                                                                 tGATTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGAAATTGTTAtt                             <  2:441725/140‑1 (MQ=255)                                                                                                   aTTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGAAATTGTTa                               <  2:607885/136‑1 (MQ=255)                                                                                                   aTTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGAAATTGTTa                               >  1:607885/1‑136 (MQ=255)                                                                                                   aTTGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGAAATTGTTATTTa                           >  2:365137/1‑140 (MQ=255)                                                                                                    ttGAACAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGAAATTGTTATTTAg                          <  1:567370/140‑1 (MQ=255)                                                                                                        aCAAAGGGAAAGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGAAATTGTTATTTAGATaa                      >  1:524366/1‑140 (MQ=255)                                                                                                                 aaGCAATTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGAAATTGTTATTTAGATAAATATGGCGa             >  2:4899/1‑140 (MQ=255)                                                                                                                     aaTTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGAAATTGTTATTTAGATAAATATGGCGATGCa         <  1:700334/140‑1 (MQ=255)                                                                                                                      aTTCTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGAAATTGTTATTTAGATAAATATGGCGATGCaa        <  1:555858/140‑1 (MQ=255)                                                                                                                         cTGACAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGAAATTGTTATTTAGATAAATATGGCGATGCAAata     >  2:313415/1‑140 (MQ=255)                                                                                                                            aCAGCG‑CCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGAAATTGTTATTTAGATAAATATGGCGATGCAAATatca  <  1:224786/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       ATTTAAATGAACCAGAGATAATATTTTTACATGCCGATCTTCAGCAATACTTAAGCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCCCAGGAAGTGATTGAACAAAGGGAAAGCAATTCTGACAGCGCCCCCTATACGTTATTAAAAAACCATGCTGACAGATTTAAAAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGGCAAACAGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCA  >  NC_000913/2383587‑2383850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑