Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,194,843	(G)6→7	noncoding (23/145 nt)	sibD ←	small RNA SibD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,194,837	1	.	G	100.0%	136.1 / NA	39	noncoding (29/145 nt)	sibD	small RNA SibD
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (22/17);  total (22/17)

AGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGA‑GGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGG  >  NC_000913/3194704‑3194971                                                                                                                                       |                                                                                                                                       agcagGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGA‑gggggg                                                                                                                                  <  1:284437/140‑1 (MQ=255)        ggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAAt                                                                                                                            >  1:7317/1‑140 (MQ=255)        ggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAAt                                                                                                                            <  2:7317/140‑1 (MQ=255)         gCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAAtc                                                                                                                           >  1:728632/1‑140 (MQ=255)         gCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAAtc                                                                                                                           <  1:65581/140‑1 (MQ=255)               aCGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATcctccct                                                                                                                     <  2:48241/140‑1 (MQ=255)                       gCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTg                                                                                                             <  2:483931/140‑1 (MQ=255)                       gCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTg                                                                                                             <  1:84591/140‑1 (MQ=255)                       gCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTg                                                                                                             <  2:50111/140‑1 (MQ=255)                           cTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTcc                                                                                                         <  2:728632/140‑1 (MQ=255)                            tCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCct                                                                                                        >  1:27744/1‑140 (MQ=255)                             cccGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCctt                                                                                                       >  1:625183/1‑140 (MQ=255)                                     agGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAg                                                                                               >  1:325329/1‑140 (MQ=255)                                              tACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaaaa                                                                                      >  1:258110/1‑140 (MQ=255)                                                        aaGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGt                                                                            >  1:132908/1‑140 (MQ=255)                                                                     aaGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCa                                                               >  1:104610/1‑140 (MQ=255)                                                                       gCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCAtt                                                             >  1:494248/1‑140 (MQ=255)                                                                        cTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTa                                                            <  2:27744/140‑1 (MQ=255)                                                                           gTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAaca                                                         >  2:64915/1‑140 (MQ=255)                                                                            tcatcaTACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAacac                                                        >  1:201735/1‑140 (MQ=255)                                                                             catcatACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAAcaca                                                       <  2:132908/140‑1 (MQ=255)                                                                             catcatACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAAcaca                                                       <  2:325329/140‑1 (MQ=255)                                                                             catcatACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAAcaca                                                       <  2:604972/140‑1 (MQ=255)                                                                                   aCTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATccc                                                 >  1:288930/1‑140 (MQ=255)                                                                                       aTTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAac                                                          >  1:707029/1‑127 (MQ=255)                                                                                       aTTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAac                                                          <  2:707029/127‑1 (MQ=255)                                                                                           tgttgtTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCaa                                         >  1:582837/1‑140 (MQ=255)                                                                                             ttgttACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCaaaa                                       >  2:296557/1‑140 (MQ=255)                                                                                               gttACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAg                                     >  1:153388/1‑140 (MQ=255)                                                                                               gttACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAg                                     >  1:425659/1‑140 (MQ=255)                                                                                                 tACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGcc                                   >  2:488244/1‑140 (MQ=255)                                                                                                      gTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGa                              <  1:488244/140‑1 (MQ=255)                                                                                                      gTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGa                              <  1:296557/140‑1 (MQ=255)                                                                                                          gTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGAtttt                          <  1:64915/140‑1 (MQ=255)                                                                                                                   cTTATTAACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTg                 >  2:439159/1‑140 (MQ=255)                                                                                                                     tattaACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCg               <  2:494248/140‑1 (MQ=255)                                                                                                                      attaACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCg               >  2:117043/1‑139 (MQ=255)                                                                                                                      attaACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCg               <  1:117043/139‑1 (MQ=255)                                                                                                                      attaACAGCCAATCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGt              >  2:119506/1‑140 (MQ=255)                                                                                                                                  tCAGAGGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAgg  >  2:514919/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       AGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGA‑GGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGG  >  NC_000913/3194704‑3194971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑