| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,379,266 | (C)7→9 | coding (732/1128 nt) | zapE ← | cell division factor ZapE with ATPase activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,379,259 | 1 | . | C | 89.7% | 85.1 / 4.0 | 30 | coding (739/1128 nt) | zapE | cell division factor ZapE with ATPase activity |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (11/15); total (13/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 3,379,259 | 2 | . | C | 90.0% | 90.8 / 4.0 | 30 | coding (739/1128 nt) | zapE | cell division factor ZapE with ATPase activity |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (12/15); total (13/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCA > NC_000913/3379121‑3379384 || gTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑c < 2:315961/140‑1 (MQ=255) gagCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑ccccc < 2:714744/140‑1 (MQ=255) cgcAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑ccccc‑‑ccc > 1:682936/1‑139 (MQ=255) cgcAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑ccccc‑‑ccc > 1:241697/1‑139 (MQ=255) cAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑ccccc‑‑ccccg < 1:10128/140‑4 (MQ=255) aaTATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑ccccc‑‑ccccgc > 1:31395/1‑136 (MQ=255) aaTATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑ccccc‑‑ccccgc > 1:490469/1‑136 (MQ=255) gTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAgcgc < 1:596813/140‑1 (MQ=255) gCTGACTGCGGGCGTCGACCCCCAGCGTAGTAAAAGAGACCGCCAGCGTTTGGTTCTCGACGCCCTTTTTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAAcc < 2:196294/140‑1 (MQ=255) cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGATCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCa > 1:687937/1‑140 (MQ=255) aCTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑ccccc‑‑ccccgc < 1:226137/125‑5 (MQ=255) aCTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑ccccc‑‑ccccgc < 1:74224/125‑5 (MQ=255) aCTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑ccccc‑‑ccccgc > 2:226137/1‑121 (MQ=255) aCTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑ccccc‑‑ccccgc > 2:74224/1‑121 (MQ=255) cTGCGGGCGTCGCCGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGt < 2:712478/140‑1 (MQ=255) cTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGt < 2:235295/140‑1 (MQ=255) tCGACCCACGGTGTAGTAAAAGAGACCGCCGGCGTCTGGTTCTCGACGCCAATTGTTGCTAATGGCCGAGGGTTGATTTCTAACGCCGGTGATTTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCAtt < 2:31395/140‑1 (MQ=255) cGCACAGCGTAGTAAAAAAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TTC‑CCCCC‑‑CCCCCCGCGCCCACCATAGTTTATCCCTTTgcg > 2:205876/1‑140 (MQ=255) aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt > 2:425341/1‑140 (MQ=255) aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑CCCCCCACCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt > 1:474429/1‑140 (MQ=255) cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt < 2:382058/126‑1 (MQ=255) cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt > 1:382058/1‑126 (MQ=255) cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg > 2:622554/1‑140 (MQ=255) cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg > 2:131949/1‑140 (MQ=255) ggCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaa < 1:556202/139‑1 (MQ=255) tCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc > 2:155686/1‑140 (MQ=255) ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑CCCCCCACCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATg < 2:474429/140‑1 (MQ=255) aCGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc < 2:687937/140‑1 (MQ=255) ctaTTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag < 2:312178/138‑1 (MQ=255) ccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag > 1:429279/1‑140 (MQ=255) cATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagt < 1:205876/140‑1 (MQ=255) ttgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCTGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtga > 2:10777/1‑140 (MQ=255) aTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCCCCCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc > 1:571376/1‑140 (MQ=255) ggTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAAt > 1:132249/1‑140 (MQ=255) ttCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACAcc < 1:622554/140‑1 (MQ=255) aCGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGt > 2:151101/1‑140 (MQ=255) cGTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTc < 1:425341/140‑1 (MQ=255) gTCGGTGAATTTTCTCGTTCCC‑‑‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTcc < 2:682936/140‑1 (MQ=255) gTCGGTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTcc < 2:241697/140‑1 (MQ=255) ggTGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACg < 2:132249/140‑1 (MQ=255) tGAATTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGtt < 1:131949/140‑1 (MQ=255) aaTTTTCTCGTT‑‑TCC‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCa < 1:151101/140‑1 (MQ=255) || GTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑CCCCC‑‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCA > NC_000913/3379121‑3379384 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |