| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,392,261 | (A)8→9 | intergenic (+112/‑99) | orn → / → glyV | oligoribonuclease/tRNA‑Gly |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,392,253 | 1 | . | A | 97.3% | 126.1 / ‑4.6 | 38 | intergenic (+104/‑107) | orn/glyV | oligoribonuclease/tRNA‑Gly |
| Reads supporting (aligned to +/- strand): ref base . (1/0); major base A (15/21); minor base T (1/0); total (17/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGC‑AAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGT > NC_000913/4392114‑4392379 | cTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGc < 1:726080/140‑1 (MQ=255) cTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGC‑aaaa > 2:292761/1‑140 (MQ=255) gcgAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGC‑aaaaaaaaacttt > 1:286642/1‑135 (MQ=255) gcgAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGC‑aaaaaaaaacttt > 1:677127/1‑135 (MQ=255) gcgAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGATGATAATTTCAGCACTTGAGATAAAAACGC‑aaaaaaaaacttt > 1:119470/1‑135 (MQ=255) cgAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTACTGATAATTTCAGCACTTGAGATAAAAACGC‑AAAAAAAAActttt > 2:586436/1‑140 (MQ=255) aTTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTggg < 1:292761/140‑1 (MQ=255) ttttATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTgggg < 2:468670/140‑1 (MQ=255) tCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTg < 2:295632/140‑1 (MQ=255) aaGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCa < 2:286642/140‑1 (MQ=255) aGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCag > 1:77163/1‑140 (MQ=255) gCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCaga > 2:37996/1‑140 (MQ=255) tGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAAAGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAgg > 2:271872/1‑140 (MQ=255) tAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGTGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGa > 1:319925/1‑140 (MQ=255) tAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACGTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATGAAAACGCTAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGa > 2:643710/1‑140 (MQ=255) tttttCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGAt < 2:456475/140‑1 (MQ=255) cACGTAAGCGCCGGAATCCACTTGCGGTGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATaa < 2:319925/140‑1 (MQ=255) aCGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAAt < 2:77163/140‑1 (MQ=255) gTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATgc > 2:255617/1‑140 (MQ=255) tAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATgcg < 1:37996/140‑1 (MQ=255) tAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATgcg < 1:255617/140‑1 (MQ=255) gAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGtata < 2:83416/126‑1 (MQ=255) gAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGtata > 1:83416/1‑126 (MQ=255) gAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTa > 1:408092/1‑140 (MQ=255) aaTCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTaa < 2:587060/140‑1 (MQ=255) tCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAacg < 2:408092/140‑1 (MQ=255) cggcgTGATTTTGACGCTAAATTGAACGTCTTGATGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATg < 2:119470/140‑1 (MQ=255) gcgTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCg > 1:3732/1‑140 (MQ=255) aCGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGaa > 1:289349/1‑140 (MQ=255) aCGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGaa > 1:488743/1‑140 (MQ=255) tAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCa < 2:289349/140‑1 (MQ=255) aCGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAag < 2:554279/140‑1 (MQ=255) aCGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAag < 2:617527/140‑1 (MQ=255) tCTTGCTGATAATTTCAGCACTTGAGATAAAAAAGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAagtag < 1:271872/140‑1 (MQ=255) tGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTAc > 2:232162/1‑140 (MQ=255) tGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTAc > 1:182709/1‑140 (MQ=255) tttCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATa < 2:677127/140‑1 (MQ=255) tCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGc > 2:427065/1‑140 (MQ=255) tCAGCACTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGc < 2:3732/140‑1 (MQ=255) aCTTGAGATAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGt > 1:668456/1‑140 (MQ=255) gagaTAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGt > 2:48971/1‑140 (MQ=255) gagaTAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGt < 1:682261/140‑1 (MQ=255) gagaTAAAAACGCAAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGt < 2:668456/140‑1 (MQ=255) | CTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGC‑AAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGT > NC_000913/4392114‑4392379 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |