| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 685,941 | (C)6→7 | coding (729/741 nt) | gltJ ← | glutamate/aspartate ABC transporter membrane subunit GltJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 685,935 | 1 | . | C | 96.5% | 90.9 / ‑5.7 | 30 | G245G (GGC→GGG) | gltJ | glutamate/aspartate ABC transporter membrane subunit GltJ |
| Reads supporting (aligned to +/- strand): ref base . (1/0); major base C (12/16); minor base T (1/0); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCATCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTG‑CCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGG > NC_000913/685797‑686053 | gCATCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGTGGAGGGACAATGGGACTCCAGGCAAAATCGTACATTA‑TTTG‑c > 2:679419/1‑140 (MQ=255) tCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTG‑cccc < 1:36107/140‑1 (MQ=255) cacccGCCAGCATCGTGCCCCAAAAAATCCAAACCACAACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTG‑cccc < 2:268432/137‑1 (MQ=255) cGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTG‑ccccccca < 1:189195/140‑3 (MQ=255) cGCCAGCATCGTGCCCCAAAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTG‑ccccccca < 1:679419/140‑3 (MQ=255) ccAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTG‑cccccccatg > 1:39950/1‑136 (MQ=255) cAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTG‑cccccccatgt > 2:586102/1‑135 (MQ=255) gCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCcaggca > 2:472937/1‑140 (MQ=255) caAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGaa > 1:475322/1‑140 (MQ=255) tCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCa > 2:312662/1‑140 (MQ=255) tCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCa > 2:417519/1‑140 (MQ=255) cACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCaa < 1:432050/140‑1 (MQ=255) aCTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAAc < 1:312662/140‑1 (MQ=255) gccgTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAGCTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGt > 1:256844/1‑140 (MQ=255) gccgTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGt < 2:475322/140‑1 (MQ=255) tGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTTCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGtcatc > 1:221929/1‑140 (MQ=255) gACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGGCAATGGAACTCCAGTCAAACTCGTACATTATTTTC‑CCCCCCACGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGgcatca > 2:348385/1‑140 (MQ=255) aCGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCAc < 1:472937/140‑1 (MQ=255) cGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCAcc < 1:11326/140‑1 (MQ=255) cGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCAcc < 1:191477/140‑1 (MQ=255) gTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAg < 1:417519/140‑1 (MQ=255) gggTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATaaaa > 1:418679/1‑140 (MQ=255) tGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCg < 2:418679/140‑1 (MQ=255) tGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCg < 1:596541/140‑1 (MQ=255) tCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAGCTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTa < 2:256844/140‑1 (MQ=255) aCCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAAt < 2:39950/140‑1 (MQ=255) ccAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGTCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATc > 2:283705/1‑140 (MQ=255) cAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCa < 1:586102/140‑1 (MQ=255) cGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAAcaca > 1:708938/1‑140 (MQ=255) tCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCa > 2:290529/1‑133 (MQ=255) tCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCa < 1:290529/133‑1 (MQ=255) gaagggaCAATGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCggg > 1:302497/1‑139 (MQ=255) tGGAACTCCAGTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTcc < 1:283705/140‑1 (MQ=255) gTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCCGGCATggg > 1:402542/1‑140 (MQ=255) gTCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATggg < 2:708938/140‑1 (MQ=255) tCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGa > 2:276769/1‑125 (MQ=255) tCAAACTCGTACATTA‑TTTGCCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGa < 1:276769/125‑1 (MQ=255) | GCATCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTA‑TTTG‑CCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGG > NC_000913/685797‑686053 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |