| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,292,147 | (A)5→6 | coding (691/909 nt) | galU → | UTP‑‑glucose‑1‑phosphate uridylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,292,142 | 1 | . | A | 93.2% | 145.3 / 2.7 | 44 | coding (686/909 nt) | galU | UTP‑‑glucose‑1‑phosphate uridylyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base A (18/23); total (20/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01 | |||||||||||
TGCAAAGGCGTTGAATTAGCGCCGGGTGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGC‑AAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGTAATAAATTAGGTTACAT > NC_000913/1292003‑1292274 | tGCAAAGGCGTTGAATTAGCGCCGGGTGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGc > 1:406157/1‑140 (MQ=255)tGCAAAGGCGTTGAATTAGCGCCGGGAGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGc > 2:601681/1‑140 (MQ=255) gCAAAGGCGTTGAATTAGCGCCGGGTGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGC‑a < 2:669076/140‑1 (MQ=255) gCGTTGAATTAGCGCCGGGTGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGC‑aaaaaac > 1:582744/1‑138 (MQ=255) gCGTTGAATTAGCGCCGGGTGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGC‑aaaaaac > 1:501077/1‑138 (MQ=255) ggTGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGTATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGTCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGtg > 2:501082/1‑140 (MQ=255) gTGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGtga < 1:729331/140‑1 (MQ=255) aTGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGC‑AAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCAc > 2:694038/1‑140 (MQ=255) tggttggtGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCAc < 2:21235/140‑1 (MQ=255) gttggtGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCg < 1:601681/140‑1 (MQ=255) ttggtGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGa < 2:207313/140‑1 (MQ=255) ggtGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACg < 2:370847/140‑1 (MQ=255) ggtGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGC‑AAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGc < 1:694038/140‑1 (MQ=255) aGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGat < 2:406157/140‑1 (MQ=255) aGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGaa < 2:501077/140‑1 (MQ=255) aGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGaa < 2:582744/140‑1 (MQ=255) cgcCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGt > 2:715552/1‑140 (MQ=255) tCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAg > 1:126677/1‑140 (MQ=255) cTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGc < 2:580524/140‑1 (MQ=255) tAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGcc < 1:597091/140‑1 (MQ=255) aaTCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCt < 2:698868/140‑1 (MQ=255) tctcGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAg > 2:536584/1‑135 (MQ=255) tctcGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAg < 1:536584/135‑1 (MQ=255) cGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCat > 1:650093/1‑140 (MQ=255) cTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGTCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCatat > 2:637844/1‑140 (MQ=255) cTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCatat > 2:530610/1‑140 (MQ=255) tgtgGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGc < 2:65917/127‑1 (MQ=255) tgtgGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGc > 1:65917/1‑127 (MQ=255) tgtgGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGaaa > 1:611923/1‑140 (MQ=255) gtgGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAg > 1:430133/1‑140 (MQ=255) ggTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGa < 2:611923/140‑1 (MQ=255) cGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAaga > 1:38019/1‑140 (MQ=255) ttACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGa > 1:240238/1‑135 (MQ=255) ttACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGa < 2:240238/135‑1 (MQ=255) ttACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGc > 1:707130/1‑140 (MQ=255) ttACGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGc > 1:315084/1‑140 (MQ=255) aCGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGaa > 2:565363/1‑134 (MQ=255) aCGTACTTAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGaa < 1:565363/134‑1 (MQ=255) ttAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTg < 2:315084/140‑1 (MQ=255) tAGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGc < 1:51720/140‑1 (MQ=255) aGCGCGGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCg > 2:215452/1‑140 (MQ=255) cgcgGATATTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGt < 1:715552/140‑1 (MQ=255) cgcgGATATTTGGCCGTTGCTGGC‑AAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGta > 2:116877/1‑140 (MQ=255) tatTTGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGTAATaaa < 1:501082/140‑1 (MQ=255) tttGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGTAATAAAtt < 1:530610/140‑1 (MQ=255) tGGCCGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCATCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGTAATAAATTAg > 1:7142/1‑140 (MQ=255) ccGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAACAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGTAATAAATTAGGtt < 2:650093/140‑1 (MQ=255) ccGTTGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGTAATAAATTAGGtt < 1:215452/140‑1 (MQ=255) tGCTGGCAAAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGTAATAAATTAGGTTACAt > 1:549955/1‑140 (MQ=255) | TGCAAAGGCGTTGAATTAGCGCCGGGTGAAAGCGTACCGATGGTTGGTGTGGTAGAAAAACCGAAAGCGGATGTTGCGCCGTCTAATCTCGCTATTGTGGGTCGTTACGTACTTAGCGCGGATATTTGGCCGTTGCTGGC‑AAAAACCCCTCCGGGAGCTGGTGATGAAATTCAGCTCACCGACGCAATTGATATGCTGATCGAAAAAGAAACGGTGGAAGCCTATCATATGAAAGGGAAGAGCCATGACTGCGGTAATAAATTAGGTTACAT > NC_000913/1292003‑1292274 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |