Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,638,026 (C)6→7 coding (627/1179 nt) bamB ← outer membrane protein assembly factor BamB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,638,0201.C92.2% 158.8 / 6.5 51coding (633/1179 nt)bamBouter membrane protein assembly factor BamB
Reads supporting (aligned to +/- strand):  ref base . (2/2);  new base C (23/24);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

CGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCG‑‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACT  >  NC_000913/2637884‑2638154
                                                                                                                                          |                                                                                                                                       
cGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCG‑‑ccc                                                                                                                                     >  2:149396/1‑140 (MQ=255)
cGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCG‑‑ccc                                                                                                                                     >  1:471457/1‑140 (MQ=255)
            cgacgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGc                                                                                                                          >  2:97266/1‑140 (MQ=255)
               cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGc                                                                                                                       >  2:330690/1‑140 (MQ=255)
                       gtgtCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAg                                                                                                               >  2:60680/1‑140 (MQ=255)
                       gtgtCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAg                                                                                                               >  2:165296/1‑140 (MQ=255)
                       gtgtCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAg                                                                                                               >  1:236123/1‑140 (MQ=255)
                              cATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGt                                                                                                        <  1:149396/140‑1 (MQ=255)
                                   cTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGgcg                                                                                                   <  1:444115/140‑1 (MQ=255)
                                   cTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGgcg                                                                                                   >  2:208473/1‑140 (MQ=255)
                                   cTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCG‑‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGt                                                                                                        >  1:218379/1‑134 (MQ=255)
                                   cTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCG‑‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGt                                                                                                        <  2:218379/134‑1 (MQ=255)
                                        aCGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGAc                                                                                              >  2:381006/1‑140 (MQ=255)
                                         cGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACt                                                                                             >  1:374408/1‑140 (MQ=255)
                                          ggTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTc                                                                                            <  1:29800/140‑1 (MQ=255)
                                          ggTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTc                                                                                            <  2:471457/140‑1 (MQ=255)
                                           gTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCg                                                                                           >  1:71202/1‑140 (MQ=255)
                                           gTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCg                                                                                           >  1:696302/1‑140 (MQ=255)
                                            tCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGc                                                                                          <  1:165296/140‑1 (MQ=255)
                                            tCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGc                                                                                          <  2:314400/140‑1 (MQ=255)
                                            tCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGc                                                                                          <  1:60680/140‑1 (MQ=255)
                                                  tCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCa                                                                                    <  2:187634/140‑1 (MQ=255)
                                                    ggTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCaaa                                                                                  <  1:330690/140‑1 (MQ=255)
                                                     gTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGgcg                                                                                                   <  1:508011/122‑1 (MQ=255)
                                                     gTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGgcg                                                                                                   >  2:508011/1‑122 (MQ=255)
                                                      tAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAaga                                                                                >  1:122329/1‑140 (MQ=255)
                                                      tAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAaga                                                                                >  1:227653/1‑140 (MQ=255)
                                                           ccGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCg                                                                           <  2:214587/140‑1 (MQ=255)
                                                              gTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAg                                                                        >  1:92411/1‑140 (MQ=255)
                                                              gTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCG‑‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAgg                                                                       >  2:76485/1‑140 (MQ=255)
                                                                  ccTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCG‑‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCata                                                                   <  1:76485/140‑1 (MQ=255)
                                                                    tGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCatat                                                                  <  2:122329/140‑1 (MQ=255)
                                                                     gggAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATc                                                                 <  2:470592/140‑1 (MQ=255)
                                                                           tACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGCCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGc                                                                      >  1:534902/1‑130 (MQ=255)
                                                                           tACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGCCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGc                                                                      <  2:534902/130‑1 (MQ=255)
                                                                             cGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTaa                                                         <  1:413470/140‑1 (MQ=255)
                                                                               ctgctgCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACt                                                       <  1:208473/140‑1 (MQ=255)
                                                                                tgctgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTg                                                      >  1:313468/1‑140 (MQ=255)
                                                                                  ctgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTc                                                    >  2:257294/1‑140 (MQ=255)
                                                                                        aaTCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTg                                              <  1:97266/140‑1 (MQ=255)
                                                                                                       ttCCATCAGCACTGCGCTGACGCGACCAATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGc                                >  2:188439/1‑140 (MQ=255)
                                                                                                       ttCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCt                               <  1:674753/140‑1 (MQ=255)
                                                                                                       ttCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCt                               >  2:699613/1‑140 (MQ=255)
                                                                                                        tCCATCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCtt                              <  2:313468/140‑1 (MQ=255)
                                                                                                            tCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGtt                          <  1:257294/140‑1 (MQ=255)
                                                                                                            tCAGCACTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGtt                          >  1:629696/1‑140 (MQ=255)
                                                                                                                 aCTGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAgcg                     >  1:261631/1‑140 (MQ=255)
                                                                                                                   tGCGCTGACGCGACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCt                   <  2:374408/140‑1 (MQ=255)
                                                                                                                           cgcgACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTg           <  2:236123/140‑1 (MQ=255)
                                                                                                                            gcgACC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGa          <  2:92411/140‑1 (MQ=255)
                                                                                                                               aCC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCg       >  1:73651/1‑140 (MQ=255)
                                                                                                                               aCC‑ATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCg       <  2:116891/140‑1 (MQ=255)
                                                                                                                                    ttATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTAc   <  1:699613/140‑1 (MQ=255)
                                                                                                                                     tATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACt  >  1:350477/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                       
CGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACC‑ATTATCG‑‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACT  >  NC_000913/2637884‑2638154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑