Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	35,645	C→T	W173* (TGG→TAG)	caiD ←	crotonobetainyl‑CoA hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	35,645	0	C	T	100.0%	67.4 / NA	20	W173* (TGG→TAG)	caiD	crotonobetainyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/13);  total (7/13)

GCATTTCGCTGGTGGTGCGGTAGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCCAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAGGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGTTTGGC  >  NC_000913/35511‑35769                                                                                                                                       |                                                                                                                             gCATTTCGCTGGTGGTGCGGTAGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGc                                                                                                                         <  1:424717/140‑1 (MQ=255)  cATTTCGCTGGTGGTGCGGTAGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCa                                                                                                                        >  1:677599/1‑140 (MQ=255)   aTTTCGCTGGTGGTGCGGTAGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAg                                                                                                                       <  2:103253/140‑1 (MQ=255)                tgCGGTAGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAGCGCCTCTTCTgcg                                                                                                          <  2:22899/140‑1 (MQ=255)                      aGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAGCGcc                                                                                                                   <  1:289317/125‑1 (MQ=255)                      aGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAGCGcc                                                                                                                   >  2:289317/1‑125 (MQ=255)                             tttCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGc                                                                                             <  2:338100/140‑1 (MQ=255)                                    gccgcAATCGCCATCGGGGGGCTGTTAACCAGCTGCTGAGCCTGTTCGCAGCCGTTATCCAGCAGTTCCGCCGGGCTAACCGCGCGCGTGATTATGCCGTATCGCAAGCCCTCTTCTCCGCCCATTCGACTGCCGGtgat                                                                                      <  2:723959/140‑1 (MQ=255)                                    gccgcAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGtcat                                                                                      <  2:677599/140‑1 (MQ=255)                                     ccgcAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGtcatc                                                                                     >  2:192072/1‑140 (MQ=255)                                     ccgcAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGtcat                                                                                      <  1:688674/139‑1 (MQ=255)                                     ccgcAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGtcat                                                                                      >  2:371956/1‑139 (MQ=255)                                     ccgcAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGtcat                                                                                      <  1:371956/139‑1 (MQ=255)                                     ccgcAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGtcat                                                                                      >  2:688674/1‑139 (MQ=255)                                       gcAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCAc                                                                                   <  2:624933/140‑1 (MQ=255)                                         aaTCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCa                                                                                 <  2:211424/140‑1 (MQ=255)                                              ccAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCa                                                                            <  1:550124/140‑1 (MQ=255)                                                                    ctgctgAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAGGa                                                      >  2:178756/1‑140 (MQ=255)                                                                                             tCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAGGATCTTCGGCAGACGCAGCACAccgcc                             <  2:564521/140‑1 (MQ=255)                                                                                              ccATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCTAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAGGATCTTCGGCAGACGCAGCACAccgccg                            <  1:192072/140‑1 (MQ=255)                                                                                                                        cgGTTGACTATCCCCTAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAGGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGTTTGGc  >  1:179229/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                             GCATTTCGCTGGTGGTGCGGTAGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCCAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAGGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGTTTGGC  >  NC_000913/35511‑35769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑