Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	175,397	G→A	V97V (GTG→GTA)	clcA →	chloride:H(+) antiporter ClcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	175,397	0	G	A	100.0%	158.4 / NA	47	V97V (GTG→GTA)	clcA	chloride:H(+) antiporter ClcA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/21);  total (26/21)

GACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGG  >  NC_000913/175266‑175536                                                                                                                                    |                                                                                                                                            gACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATa                                                                                                                                     >  2:485409/1‑140 (MQ=255)           tCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGaa                                                                                                                           <  1:315288/140‑1 (MQ=255)                         aaCCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCggg                                                                                                             <  2:502771/140‑1 (MQ=255)                              aCGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATcc                                                                                                        >  1:360434/1‑140 (MQ=255)                                     ggggCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATcc                                                                                                        >  1:187770/1‑133 (MQ=255)                                     ggggCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATcc                                                                                                        <  2:187770/133‑1 (MQ=255)                                         cgcTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGaa                                                                                             >  1:324520/1‑140 (MQ=255)                                             ggTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAgggg                                                                                         <  2:58388/140‑1 (MQ=255)                                             ggTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAgggg                                                                                         <  1:361444/140‑1 (MQ=255)                                              gTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGgc                                                                                        <  2:128296/140‑1 (MQ=255)                                                 cATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCt                                                                                     <  1:485409/140‑1 (MQ=255)                                                      tgctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAg                                                                                >  2:474474/1‑140 (MQ=255)                                                        ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGAt                                                                              >  1:409001/1‑140 (MQ=255)                                                        ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGAt                                                                              >  2:165064/1‑140 (MQ=255)                                                           aTAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCaa                                                                           >  1:28450/1‑140 (MQ=255)                                                           aTAATTATCCGCTTCTGTTAACCGTCGCTTTTCCCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCaa                                                                           >  2:580383/1‑140 (MQ=255)                                                                  tCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGaa                                                                                 <  2:214869/127‑1 (MQ=255)                                                                  tCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGaa                                                                                 >  1:214869/1‑127 (MQ=255)                                                                  tCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCg                                                                    >  1:144776/1‑140 (MQ=255)                                                                  tCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCg                                                                    >  1:614081/1‑140 (MQ=255)                                                                  tCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCg                                                                    >  1:69741/1‑140 (MQ=255)                                                                  tCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCg                                                                    >  2:447916/1‑140 (MQ=255)                                                                       ttCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGc                                                               >  1:355009/1‑140 (MQ=255)                                                                       ttCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGc                                                               >  1:656600/1‑140 (MQ=255)                                                                          tGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCtgg                                                            >  1:546283/1‑140 (MQ=255)                                                                            ttAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCtggtg                                                          <  1:300932/140‑1 (MQ=255)                                                                            ttAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCtggtg                                                          >  2:76151/1‑140 (MQ=255)                                                                                   tCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGc                                                        <  2:100292/135‑1 (MQ=255)                                                                                   tCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGc                                                        >  1:100292/1‑135 (MQ=255)                                                                                   tCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTa                                                   >  2:135349/1‑140 (MQ=255)                                                                                   tCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTa                                                   >  1:643565/1‑140 (MQ=255)                                                                                   tCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTa                                                   >  1:16764/1‑140 (MQ=255)                                                                                    cGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTAt                                                  >  1:11275/1‑140 (MQ=255)                                                                                       ttttCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGc                                               <  2:409001/140‑1 (MQ=255)                                                                                                ttCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGt                                      >  2:633356/1‑140 (MQ=255)                                                                                                ttCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGt                                      >  2:716010/1‑140 (MQ=255)                                                                                                      ggTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTg                                <  2:28450/140‑1 (MQ=255)                                                                                                       gTGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTgg                               <  1:716010/140‑1 (MQ=255)                                                                                                        tGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGc                              <  2:324520/140‑1 (MQ=255)                                                                                                        tGCTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGc                              <  2:546283/140‑1 (MQ=255)                                                                                                          cTGGCGATGTTTGGCTACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCgg                            <  2:360434/140‑1 (MQ=255)                                                                                                                         tACTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTcgg             >  2:523971/1‑140 (MQ=255)                                                                                                                           cTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTcggcg           <  2:16764/140‑1 (MQ=255)                                                                                                                           cTTTTTGGTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTcggcg           <  1:580383/140‑1 (MQ=255)                                                                                                                            tttttGGTACGCAAATACGCGCCGGAAGCAGGAGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGACGGTGAAGTTCTTTGGCGGGCTGGGGACACTcggcgg          <  2:643565/140‑1 (MQ=255)                                                                                                                                 ggTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCa     <  2:355009/140‑1 (MQ=255)                                                                                                                                 ggTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCa     <  2:656600/140‑1 (MQ=255)                                                                                                                                 ggTACGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCa     <  1:165064/140‑1 (MQ=255)                                                                                                                                    gcgcAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAGGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATgg  <  2:11275/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                            GACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGG  >  NC_000913/175266‑175536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑