Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,118,539	A→G	F192L (TTC→CTC)	yghJ ←	putative lipoprotein YghJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,118,539	0	A	G	100.0%	157.9 / NA	45	F192L (TTC→CTC)	yghJ	putative lipoprotein YghJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/19);  total (26/19)

GTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCA  >  NC_000913/3118403‑3118675                                                                                                                                         |                                                                                                                                         gTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGt                                                                                                                                       >  2:692351/1‑140 (MQ=255)   aTGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCa                                                                                                                                     >  1:629982/1‑140 (MQ=255)     gAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGa                                                                                                                                   <  1:692351/140‑1 (MQ=255)       gTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACa                                                                                                                                 >  2:127905/1‑140 (MQ=255)          gATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAAc                                                                                                                              <  1:563724/140‑1 (MQ=255)                           cGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCg                                                                                                             <  1:330306/140‑1 (MQ=255)                                        tCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGc                                                                                                       >  1:170738/1‑133 (MQ=255)                                        tCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGc                                                                                                       <  2:170738/133‑1 (MQ=255)                                        tCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACt                                                                                                >  2:716637/1‑140 (MQ=255)                                          cACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTgg                                                                                              >  2:97773/1‑140 (MQ=255)                                            ccTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGAc                                                                                            >  1:602100/1‑140 (MQ=255)                                             ctctTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACg                                                                                           <  1:97773/140‑1 (MQ=255)                                                       aCCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGa                                                                                 >  2:702904/1‑140 (MQ=255)                                                              ttttGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCa                                                                          <  1:74279/140‑1 (MQ=255)                                                                      tctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCtttt                                                                  >  2:724632/1‑140 (MQ=255)                                                                         tCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCtt                                                               >  1:33333/1‑140 (MQ=255)                                                                                ccAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCg                                                        <  2:33333/140‑1 (MQ=255)                                                                                   gATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAg                                                     <  2:629982/140‑1 (MQ=255)                                                                                        aTTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGc                                                <  2:55675/140‑1 (MQ=255)                                                                                        aTTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGCTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGc                                                >  1:631418/1‑140 (MQ=255)                                                                                          ttGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCa                                              >  1:392568/1‑140 (MQ=255)                                                                                            gCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGt                                            >  1:54565/1‑140 (MQ=255)                                                                                                    aGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcgc                                    <  2:355838/140‑1 (MQ=255)                                                                                                     gCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGCTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGcgcg                                   <  2:631418/140‑1 (MQ=255)                                                                                                      cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGt                                  >  2:589731/1‑140 (MQ=255)                                                                                                      cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGt                                  >  2:285705/1‑140 (MQ=255)                                                                                                       gAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTc                                 >  1:330090/1‑140 (MQ=255)                                                                                                       gAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTc                                 >  1:390326/1‑140 (MQ=255)                                                                                                        aGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTcc                                >  1:481593/1‑140 (MQ=255)                                                                                                        aGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTcc                                >  1:479394/1‑140 (MQ=255)                                                                                                          tCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTc                              <  1:285705/140‑1 (MQ=255)                                                                                                           cGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCa                             >  2:165959/1‑140 (MQ=255)                                                                                                                       tctcGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGac                 <  2:390326/140‑1 (MQ=255)                                                                                                                       tctcGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGac                 <  1:165959/140‑1 (MQ=255)                                                                                                                            aTCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACttt            >  2:514434/1‑140 (MQ=255)                                                                                                                             tCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACtttt           >  2:477978/1‑140 (MQ=255)                                                                                                                                ccGAGGAGAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCa        >  2:94944/1‑140 (MQ=255)                                                                                                                                  gaggagAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAAc      <  1:589731/140‑1 (MQ=255)                                                                                                                                   aggagAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAAcc     >  2:424922/1‑140 (MQ=255)                                                                                                                                   aggagAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAAcc     >  1:594817/1‑140 (MQ=255)                                                                                                                                    ggagAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcg    <  2:330090/140‑1 (MQ=255)                                                                                                                                    ggagAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcg    <  1:716637/140‑1 (MQ=255)                                                                                                                                    ggagAGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcg    <  1:477978/140‑1 (MQ=255)                                                                                                                                     gagaGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAAc      <  2:363311/137‑1 (MQ=255)                                                                                                                                     gagaGCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAAc      >  1:363311/1‑137 (MQ=255)                                                                                                                                      agagCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcgca  <  2:594817/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCA  >  NC_000913/3118403‑3118675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑