BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,179,935	T→C	C64C (TGT→TGC)	ygiB →	DUF1190 domain‑containing protein YgiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,179,935	0	T	C	100.0%	123.8 / NA	36	C64C (TGT→TGC)	ygiB	DUF1190 domain‑containing protein YgiB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/16); total (20/16)

CCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCAT  >  NC_000913/3179800‑3180060

ccATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCacca                                                                                                                           <  2:191890/140‑1 (MQ=255)

ccATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCacca                                                                                                                           <  1:421861/140‑1 (MQ=255)

       acacCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCaccacc                                                                                                                         <  2:449761/135‑1 (MQ=255)

       acacCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCaccacc                                                                                                                         >  1:449761/1‑135 (MQ=255)

           cAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTacaac                                                                                                                >  1:437993/1‑140 (MQ=255)

             gTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTAcaacaa                                                                                                              >  1:721984/1‑140 (MQ=255)

             gTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTAcaacaa                                                                                                              >  2:50333/1‑140 (MQ=255)

              tCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAAt                                                                                                             <  2:602662/140‑1 (MQ=255)

                 ctctCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAAt                                                                                                             <  2:308502/137‑1 (MQ=255)

                 ctctCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAAt                                                                                                             >  1:308502/1‑137 (MQ=255)

                 ctctCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATgcg                                                                                                          >  2:676879/1‑140 (MQ=255)

                          ttGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAaagaa                                                                                                 <  1:50333/140‑1 (MQ=255)

                          ttGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAaagaa                                                                                                 <  1:676879/140‑1 (MQ=255)

                                cTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGaa                                                                                           >  1:278924/1‑140 (MQ=255)

                                        aTGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAGTGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTgc                                                                                   <  2:292592/140‑1 (MQ=255)

                                             ggctggctgTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACt                                                                                     >  1:218307/1‑133 (MQ=255)

                                             ggctggctgTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACt                                                                                     <  2:218307/133‑1 (MQ=255)

                                                    tgtgAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGc                                                                       <  2:721984/140‑1 (MQ=255)

                                                                gatgaAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGa                                                           >  1:130224/1‑140 (MQ=255)

                                                                         gtgtCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGc                                                  >  1:312335/1‑140 (MQ=255)

                                                                             ctctctATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGaa                                              >  1:316403/1‑140 (MQ=255)

                                                                              tctctATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAAt                                             <  1:560783/140‑1 (MQ=255)

                                                                               ctctATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAAtt                                            >  1:623344/1‑140 (MQ=255)

                                                                                    tCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTg                                       >  2:152342/1‑140 (MQ=255)

                                                                                         aTGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGt                                  <  2:130224/140‑1 (MQ=255)

                                                                                          tGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTc                                 <  2:316403/140‑1 (MQ=255)

                                                                                          tGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTc                                 >  1:699522/1‑140 (MQ=255)

                                                                                          tGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTc                                 >  2:633610/1‑140 (MQ=255)

                                                                                              gacgacTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAAtt                                            >  2:454610/1‑125 (MQ=255)

                                                                                              gacgacTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAAtt                                            <  1:454610/125‑1 (MQ=255)

                                                                                                   cTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCcagc                        <  2:278924/140‑1 (MQ=255)

                                                                                                         aGCTGCAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCAc                  >  1:529875/1‑140 (MQ=255)

                                                                                                              cAAACCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGcc             <  2:1613/140‑1 (MQ=255)

                                                                                                                aaCCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCa           >  2:676030/1‑140 (MQ=255)

                                                                                                                aaCCCAGGCAAAAGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCa           >  1:215460/1‑140 (MQ=255)

                                                                                                                         aaaaGCGCCGAATGCACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCtggcat  >  2:57906/1‑140 (MQ=255)

CCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCAT  >  NC_000913/3179800‑3180060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`