Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,198,142	T→C	G500G (GGA→GGG)	glnE ←	fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,198,142	0	T	C	100.0%	179.9 / NA	52	G500G (GGA→GGG)	glnE	fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/20);  total (32/20)

AGCAATTCTAAATAGGTGGTGCGGGTAACAATCCCCACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCA  >  NC_000913/3198008‑3198276                                                                                                                                       |                                                                                                                                       aGCAATTCTAAATAGGTGGTGCGGGTAACAATCCCCACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTcg                                                                                                                                   >  1:591733/1‑140 (MQ=255) aGCAATTCTAAATAGGTGGTGCGGGTAACAATCCCCACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTcg                                                                                                                                   >  2:178240/1‑140 (MQ=255)  gCAATTCTAAATAGGTGGTGCGGGTAACAATCCCCACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTcgc                                                                                                                                  >  1:153323/1‑140 (MQ=255)      ttCTAAATAGGTGGTGCGGGTAACAATCCCCACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGcc                                                                                                                              <  2:591733/140‑1 (MQ=255)                gtggtgCGGGTAACAATCCCCACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTgcg                                                                                                                    >  2:362915/1‑140 (MQ=255)                 tggtgCGGGTAACAATCCCCACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTgcgc                                                                                                                   <  2:153323/140‑1 (MQ=255)                  ggtgCGGGTAACAATCCCCACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCt                                                                                                                  >  1:186573/1‑140 (MQ=255)                   gtgCGGGTAACAATCCCCACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCtt                                                                                                                 >  1:455761/1‑140 (MQ=255)                   gtgCGGGTAACAATCCCCACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCACACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCtt                                                                                                                 >  1:7019/1‑140 (MQ=255)                     gCGGGTAACAATCCCCACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTAt                                                                                                               >  2:24392/1‑140 (MQ=255)                         gTAACAATCCCCACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAg                                                                                                           >  2:709387/1‑140 (MQ=255)                         gTAACAATCCCCACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAg                                                                                                           >  1:661176/1‑140 (MQ=255)                                  cccACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCg                                                                                                  >  1:364792/1‑140 (MQ=255)                                   ccaccaGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCgg                                                                                                 >  1:459180/1‑140 (MQ=255)                                    caccaGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGa                                                                                                <  2:364792/140‑1 (MQ=255)                                          cAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCgg                                                                                          >  2:63704/1‑140 (MQ=255)                                            aGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCa                                                                                        <  2:455761/140‑1 (MQ=255)                                              gCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAAt                                                                                      >  1:117120/1‑140 (MQ=255)                                                ggTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCa                                                                                    <  2:81177/140‑1 (MQ=255)                                                    aTGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGt                                                                                >  1:285873/1‑140 (MQ=255)                                                        gcgACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGAAATCAGCGTTAGc                                                                            >  2:43421/1‑140 (MQ=255)                                                          gACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCAc                                                                          <  1:63704/140‑1 (MQ=255)                                                          gACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCAc                                                                          <  1:24392/140‑1 (MQ=255)                                                              gCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGt                                                                      <  2:536818/140‑1 (MQ=255)                                                               cGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATc                                                                                     <  2:414668/124‑1 (MQ=255)                                                               cGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATc                                                                                     >  1:414668/1‑124 (MQ=255)                                                                   aCGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCg                                                                 <  2:117120/140‑1 (MQ=255)                                                                      gCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGatc                                                              >  1:43865/1‑140 (MQ=255)                                                                        aGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGatcat                                                            <  1:715119/140‑1 (MQ=255)                                                                        aGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGatcat                                                            <  2:186573/140‑1 (MQ=255)                                                                         gCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCAcc                                                                         >  1:709940/1‑126 (MQ=255)                                                                         gCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCAcc                                                                         <  2:709940/126‑1 (MQ=255)                                                                         gCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGatcatc                                                           >  2:624445/1‑140 (MQ=255)                                                                         gCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGatcatc                                                           >  2:17184/1‑140 (MQ=255)                                                                            tCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTc                                                        >  2:29820/1‑140 (MQ=255)                                                                                      cacaGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATgc                                              >  1:168827/1‑140 (MQ=255)                                                                                         aGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGcc                                           <  1:134143/140‑1 (MQ=255)                                                                                             aTCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCa                                       <  1:17184/140‑1 (MQ=255)                                                                                                    aGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGc                                  >  1:128265/1‑138 (MQ=255)                                                                                                    aGCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGc                                  <  2:128265/138‑1 (MQ=255)                                                                                                     gCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTa                               >  1:358351/1‑140 (MQ=255)                                                                                                     gCAGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTa                               <  1:404853/140‑1 (MQ=255)                                                                                                       aGATGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAgt                             >  1:603035/1‑140 (MQ=255)                                                                                                         aTGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAgtgt                           <  1:29820/140‑1 (MQ=255)                                                                                                         aTGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAgtgt                           >  2:543344/1‑140 (MQ=255)                                                                                                          tGCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTc                          >  2:295839/1‑140 (MQ=255)                                                                                                           gCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCa                         >  1:268711/1‑140 (MQ=255)                                                                                                           gCGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCa                         >  1:57325/1‑140 (MQ=255)                                                                                                            cGGCATCAGATGGTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCAt                        >  1:254197/1‑140 (MQ=255)                                                                                                                        gTCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGAAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACg            <  1:43421/140‑1 (MQ=255)                                                                                                                         tCGAGCACCTGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGc           >  1:326100/1‑140 (MQ=255)                                                                                                                                  tGACGCCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCca  <  2:603035/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       AGCAATTCTAAATAGGTGGTGCGGGTAACAATCCCCACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCA  >  NC_000913/3198008‑3198276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑