Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,211,722	T→C	F206L (TTC→CTC)	dnaG →	DNA primase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,211,722	0	T	C	100.0%	179.4 / NA	52	F206L (TTC→CTC)	dnaG	DNA primase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/26);  total (26/26)

CCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCTTTACGGTCTTTATGAAG  >  NC_000913/3211591‑3211857                                                                                                                                    |                                                                                                                                        cccccGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCAtt                                                                                                                                 <  2:315496/140‑1 (MQ=255)                aCGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGt                                                                                                                  >  1:701479/1‑140 (MQ=255)                 cGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTc                                                                                                                 >  2:108987/1‑140 (MQ=255)                 cGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTc                                                                                                                 >  2:443828/1‑140 (MQ=255)                   tCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCgg                                                                                                               <  2:104263/140‑1 (MQ=255)                       gAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGa                                                                                                           <  1:108987/140‑1 (MQ=255)                        aaGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGAt                                                                                                          >  1:484882/1‑140 (MQ=255)                             gTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGtt                                                                                                     >  1:586227/1‑140 (MQ=255)                              tttGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGttt                                                                                                    >  2:534697/1‑140 (MQ=255)                               ttGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGtttt                                                                                                   >  2:227848/1‑140 (MQ=255)                                      cAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTggcgggc                                                                                            <  1:534697/140‑1 (MQ=255)                                      cAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTggcgggc                                                                                            <  1:227848/140‑1 (MQ=255)                                        aTCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGgcgc                                                                                          <  1:25648/140‑1 (MQ=255)                                                   cGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCaa                                                                               >  1:241285/1‑140 (MQ=255)                                                     ccAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACg                                                                             >  1:448706/1‑140 (MQ=255)                                                      cAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGa                                                                            <  2:444362/140‑1 (MQ=255)                                                              gattgatGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCa                                                                    <  1:354690/140‑1 (MQ=255)                                                              gattgatGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCa                                                                    <  2:484882/140‑1 (MQ=255)                                                               attgatGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGAt                                                                           <  1:425071/132‑1 (MQ=255)                                                               attgatGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGAt                                                                           >  2:425071/1‑132 (MQ=255)                                                                   atGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGAt                                                                           >  2:576681/1‑128 (MQ=255)                                                                   atGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGAt                                                                           <  1:576681/128‑1 (MQ=255)                                                                   atGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATAc                                                               >  1:652219/1‑140 (MQ=255)                                                                   atGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATAc                                                               >  2:729992/1‑140 (MQ=255)                                                                    tGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGAt                                                                           <  2:143610/127‑1 (MQ=255)                                                                    tGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGAt                                                                           >  1:143610/1‑127 (MQ=255)                                                                             gTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATAccccc                                                                     >  1:588022/1‑124 (MQ=255)                                                                             gTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATAccccc                                                                     <  2:588022/124‑1 (MQ=255)                                                                                 gTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCAATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGa                                                 >  1:69277/1‑140 (MQ=255)                                                                                     cTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACa                                             <  1:49674/140‑1 (MQ=255)                                                                                           aTCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACAtt                                       >  1:17103/1‑140 (MQ=255)                                                                                           aTCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACAtt                                       >  2:89334/1‑140 (MQ=255)                                                                                           aTCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACAtt                                       <  2:241285/140‑1 (MQ=255)                                                                                              aGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCTGGAAACAGACATTTTc                                    >  1:119320/1‑140 (MQ=255)                                                                                                      aGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAgg                            >  2:582572/1‑140 (MQ=255)                                                                                                      aGTTACGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTAGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAgg                            >  1:502599/1‑140 (MQ=255)                                                                                                          tcGATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGgccgc                        <  1:366293/139‑1 (MQ=255)                                                                                                            gATCGTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCa                      <  2:380807/140‑1 (MQ=255)                                                                                                                gTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGgccgc                        >  2:542060/1‑134 (MQ=255)                                                                                                                gTTTCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGgccgc                        <  1:542060/134‑1 (MQ=255)                                                                                                                  ttCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCTTTa                >  1:163855/1‑140 (MQ=255)                                                                                                                  ttCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCTTTa                <  1:474449/140‑1 (MQ=255)                                                                                                                  ttCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCTTTa                >  2:478328/1‑140 (MQ=255)                                                                                                                  ttCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCTTTa                >  2:368248/1‑140 (MQ=255)                                                                                                                   tCCGCGAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCTTTAc               <  1:665322/140‑1 (MQ=255)                                                                                                                     cgcgAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCTTTACgg             <  2:17103/140‑1 (MQ=255)                                                                                                                     cgcgAGCGGGTGATGCTCCCCATTCGCGATAAACGCGGTAGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCTTTACgg             <  2:502599/140‑1 (MQ=255)                                                                                                                             ggTGATGCTCCCAATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCTTTACGGTCTTTATg     <  2:69277/140‑1 (MQ=255)                                                                                                                                gATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCTTTAc               >  1:715442/1‑127 (MQ=255)                                                                                                                                gATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCTTTAc               <  2:715442/127‑1 (MQ=255)                                                                                                                                gATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCTTTACGGTCTTTATGAAg  <  1:729992/140‑1 (MQ=255)                                                                                                                                gATGCTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCTTTACGGTCTTTATGAAg  <  1:89334/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        CCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCTTTACGGTCTTTATGAAG  >  NC_000913/3211591‑3211857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑