Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,293,659	A→G	N87S (AAC→AGC)	lpoA →	outer membrane lipoprotein ‑ activator of MrcA activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,293,659	0	A	G	100.0%	95.1 / NA	30	N87S (AAC→AGC)	lpoA	outer membrane lipoprotein ‑ activator of MrcA activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/16);  total (14/16)

GCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTT  >  NC_000913/3293526‑3293788                                                                                                                                      |                                                                                                                                  gcgcAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTTCTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGGTTAGCCAACt                                                                                                                             <  2:440875/140‑1 (MQ=255)      ggCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGc                                                                                                                        <  2:663227/140‑1 (MQ=255)             tCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACt                                                                                                                 <  1:32514/140‑1 (MQ=255)                 ccTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAAc                                                                                                             >  2:100906/1‑140 (MQ=255)                  cTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACg                                                                                                            <  1:13870/140‑1 (MQ=255)                           tCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGc                                                                                                   >  2:500799/1‑140 (MQ=255)                            cagcagATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCg                                                                                                  >  2:457415/1‑140 (MQ=255)                               cagATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTcg                                                                                               <  1:225972/140‑1 (MQ=255)                                       gcaAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGaaaa                                                                                       >  2:487584/1‑140 (MQ=255)                                                tgatgaTACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTgg                                                                              <  1:500799/140‑1 (MQ=255)                                                tgatgaTACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTgg                                                                              <  1:534732/140‑1 (MQ=255)                                                     atACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGt                                                                          <  2:446950/139‑1 (MQ=255)                                                     atACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGt                                                                          >  1:446950/1‑139 (MQ=255)                                                     atACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTa                                                                         <  2:128017/140‑1 (MQ=255)                                                          aGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTa                                                                         >  2:126260/1‑135 (MQ=255)                                                          aGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTa                                                                         <  1:126260/135‑1 (MQ=255)                                                                 aCTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTg                                                             >  2:132007/1‑140 (MQ=255)                                                                     gCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGgcgc                                                         >  2:521709/1‑140 (MQ=255)                                                                                     cGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTgg                                         <  1:211720/140‑1 (MQ=255)                                                                                      gTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTggg                                        >  2:107955/1‑139 (MQ=255)                                                                                         cACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGgcgcg                                     <  1:100906/140‑1 (MQ=255)                                                                                              gTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGGAGAGATTAAACTGGCGCAGAAAGATTTTTGTGGCGCGCaaaa                                >  2:211720/1‑140 (MQ=255)                                                                                                 aaaGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACtt                             >  1:349314/1‑140 (MQ=255)                                                                                                       ggTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGc                       <  1:132007/140‑1 (MQ=255)                                                                                                        gTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCg                      <  2:349314/140‑1 (MQ=255)                                                                                                        gTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCg                      >  2:452347/1‑140 (MQ=255)                                                                                                        gTAAAACCGGGCAGGCGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAAAACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCg                      >  2:462618/1‑140 (MQ=255)                                                                                                                       cGGTTGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGcc       >  2:408385/1‑140 (MQ=255)                                                                                                                           tGAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGAtt   <  2:353757/140‑1 (MQ=255)                                                                                                                            gAGTTGTTTAGCCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGAttt  <  1:408385/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  GCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTT  >  NC_000913/3293526‑3293788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑