Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,336,920	T→C	intergenic (‑117/+43)	ibaG ← / ← mlaB	acid stress protein IbaG/intermembrane phospholipid transport system protein MlaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,336,920	0	T	C	100.0%	181.8 / NA	51	intergenic (‑117/+43)	ibaG/mlaB	acid stress protein IbaG/intermembrane phospholipid transport system protein MlaB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/24);  total (27/24)

CTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGT  >  NC_000913/3336783‑3337048                                                                                                                                          |                                                                                                                                 ctGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGctt                                                                                                                                >  1:596226/1‑140 (MQ=255)            tattTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGTCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGc                                                                                                                     >  2:287369/1‑140 (MQ=255)            tattTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGc                                                                                                                     >  2:318748/1‑140 (MQ=255)                ttCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGtt                                                                                                                 <  2:553481/140‑1 (MQ=255)                      gggATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTTAAGcc                                                                                                           >  2:7734/1‑140 (MQ=255)                        gATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTa                                                                                                                >  2:426156/1‑133 (MQ=255)                        gATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTa                                                                                                                <  1:426156/133‑1 (MQ=255)                        gATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCat                                                                                                         >  2:529283/1‑140 (MQ=255)                                atcatcAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACCCCATATCCGGcc                                                                                                 >  2:251476/1‑140 (MQ=255)                                  catcaGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTg                                                                                               >  2:240838/1‑140 (MQ=255)                                   atcaGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGc                                                                                                            <  1:25640/126‑1 (MQ=255)                                   atcaGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGc                                                                                                            >  2:25640/1‑126 (MQ=255)                                   atcaGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAAAGCCCTATCCGGCCTGa                                                                                              >  2:4446/1‑140 (MQ=255)                                    tcaGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGaa                                                                                             >  2:657840/1‑140 (MQ=255)                                    tcaGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGaa                                                                                             >  1:47542/1‑140 (MQ=255)                                     caGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGaaa                                                                                            >  2:253124/1‑140 (MQ=255)                                           ataaACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAAttt                                                                                      >  2:87867/1‑140 (MQ=255)                                           ataaACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAAatt                                                                                      >  2:656232/1‑140 (MQ=255)                                             aaaCCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTaa                                                                                    <  1:657840/140‑1 (MQ=255)                                              aaCCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGTCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAAc                                                                                   <  1:287369/140‑1 (MQ=255)                                              aaCCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAAc                                                                                   <  1:318748/140‑1 (MQ=255)                                                  aGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAgg                                                                               <  1:612636/140‑1 (MQ=255)                                                  aGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAgg                                                                               <  1:529283/140‑1 (MQ=255)                                                  aGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAgg                                                                               >  1:366149/1‑140 (MQ=255)                                                  aGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAgg                                                                               <  2:274547/140‑1 (MQ=255)                                                  aGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCCGCCCGAAAAAATTTAAAGAgg                                                                               >  2:653571/1‑140 (MQ=255)                                                    cGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCa                                                                             >  1:231892/1‑140 (MQ=255)                                                    cGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCa                                                                             >  1:315173/1‑140 (MQ=255)                                                                gagCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATcagcag                                                                 <  1:240838/140‑1 (MQ=255)                                                                gagCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATcagcag                                                                 <  1:705725/140‑1 (MQ=255)                                                                gagCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATcagcag                                                                 <  2:231892/140‑1 (MQ=255)                                                                 agCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAgg                                                                >  2:307224/1‑140 (MQ=255)                                                                        ttGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATta                                                         <  1:656232/140‑1 (MQ=255)                                                                           cgcTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATAc                                                      >  1:464139/1‑140 (MQ=255)                                                                                    aaataaGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGcc                                             <  1:307224/140‑1 (MQ=255)                                                                                    aaataaGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGcc                                             <  2:596226/140‑1 (MQ=255)                                                                                    aaataaGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGcc                                             <  2:315173/140‑1 (MQ=255)                                                                                     aataaGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCa                                            <  1:87867/140‑1 (MQ=255)                                                                                       taaGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAgg                                          <  2:47542/140‑1 (MQ=255)                                                                                          gCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGcc                                             <  2:432655/134‑1 (MQ=255)                                                                                          gCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGcc                                             >  1:432655/1‑134 (MQ=255)                                                                                          gCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGta                                       >  1:441524/1‑140 (MQ=255)                                                                                          gCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAAATTAACGAGGCAGAAAATCAGCAGGCAAATTATACAATTTCGCCAgggga                                       >  2:74938/1‑138 (MQ=255)                                                                                          gCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGGTAAAGCCCTAACCCGCCCGGAAAAAATTAAAGAGGGAGAAAAACAACAGGCAAAATATACAAATTTGCCCgggga                                       >  2:548086/1‑138 (MQ=255)                                                                                           cAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGtat                                      <  1:253124/140‑1 (MQ=255)                                                                                              aaaGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAAAGCCATATCCCGCCCGAAAAAAATTAAAGAGGCAGAAAATCAGCAGGCAAAATATAAAAATTTGCCCGGGGATaca                                   >  2:327403/1‑140 (MQ=255)                                                                                                               tCAGACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTAccccc                  <  1:55454/140‑1 (MQ=255)                                                                                                                  gACAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCCTATCCCGCCCGAAAAAATTTAACGAGGCAGAACATCCGCAGGCAAATTATACAAATTTGCCCGGGGATACACTTTTTCGTTTACCCCCCGa               >  2:221784/1‑140 (MQ=255)                                                                                                                   aCAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGaa              <  2:441524/140‑1 (MQ=255)                                                                                                                    cAAGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAg             <  2:566917/140‑1 (MQ=255)                                                                                                                      aGGCTCGACTTGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAAATTAACGAGGCAGAACATCAGCAGGCAAAATATACAAATTTGCCAGGGGATACACTTTTTCGTTTTCCCCCTGAAGCg           >  2:55454/1‑140 (MQ=255)                                                                                                                               ttGCAGGCAGGCTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACAttgt  <  2:464139/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 CTGAATTTCATTATTTTCCATGGGATCTTCAATCATCAGTTAATAAACCAGCGAAACATCTTAGAGCAAAGTTGCGCTGGCATAAATAAGCAAAAAGCCTCGCTGATAAATCAGACAAGGCTCGACTTGCAGGCAGGTTTGCCGGACAGGCGGTTAACGCCATATCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGT  >  NC_000913/3336783‑3337048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑