Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,359,226	A→G	D10G (GAC→GGC)	gltD →	glutamate synthase subunit GltD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,359,226	0	A	G	100.0%	91.4 / NA	28	D10G (GAC→GGC)	gltD	glutamate synthase subunit GltD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/14);  total (14/14)

AATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACT  >  NC_000913/3359091‑3359360                                                                                                                                        |                                                                                                                                       aaTTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTg                                                                                                                                    <  2:647711/140‑1 (MQ=255)           ggTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTg                                                                                                                          <  1:48066/140‑1 (MQ=255)             ttAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGAt                                                                                                                        <  1:154506/140‑1 (MQ=255)                       gTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGa                                                                                                              <  2:502478/140‑1 (MQ=255)                       gTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGa                                                                                                              <  2:293887/140‑1 (MQ=255)                                tGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGa                                                                                                     >  2:466740/1‑140 (MQ=255)                                  tAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAg                                                                                                   >  2:468300/1‑140 (MQ=255)                                  tAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAg                                                                                                   >  2:181497/1‑140 (MQ=255)                                            gctgGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCaaaa                                                                                         >  1:685329/1‑140 (MQ=255)                                                 gTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAg                                                                                    >  1:610082/1‑140 (MQ=255)                                                  tCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGt                                                                                   <  1:466740/140‑1 (MQ=255)                                                                gcgcAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTAc                                                                     >  2:55768/1‑140 (MQ=255)                                                                  gcAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGa                                                                   <  2:685329/140‑1 (MQ=255)                                                                                cgTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAag                                                     >  2:524723/1‑140 (MQ=255)                                                                                             tAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAgc                                        >  1:453452/1‑140 (MQ=255)                                                                                                 gggTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAg                                    >  1:404033/1‑140 (MQ=255)                                                                                                     aGCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTg                                >  1:720520/1‑140 (MQ=255)                                                                                                      gCAACAATGAGTCAGAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGa                               <  2:720520/140‑1 (MQ=255)                                                                                                      gCAACAAGGAGTCAGAATGTTTAAAAATTTACCGCCCTACGGCGCGTTGATCCGCCAAAGAAACCGCTGAAGAGCCGCAAAATGGGGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGa                               <  2:404033/140‑1 (MQ=255)                                                                                                                    gAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCt                                 <  2:232235/124‑1 (MQ=255)                                                                                                                    gAATGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCt                                 >  1:232235/1‑124 (MQ=255)                                                                                                                     aaTGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTg                <  2:97070/140‑1 (MQ=255)                                                                                                                     aaTGTTTATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTg                <  1:186924/140‑1 (MQ=255)                                                                                                                         tttATCAATTTATCGGCCGGCAGCGCGTTGATCCGCAAAAGAACCCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGc            <  2:544747/140‑1 (MQ=255)                                                                                                                           tATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCg              >  2:411784/1‑136 (MQ=255)                                                                                                                           tATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCg              <  1:411784/136‑1 (MQ=255)                                                                                                                           tATCAATTTATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCaa          >  1:687972/1‑140 (MQ=255)                                                                                                                                   tATCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACt  <  2:610082/140‑1 (MQ=255)                                                                                                                                    aTCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGc            >  2:462961/1‑129 (MQ=255)                                                                                                                                    aTCGGCCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGc            <  1:462961/129‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       AATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGAGTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCATACT  >  NC_000913/3359091‑3359360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑