Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,546,932	T→C	V125A (GTG→GCG)	gntT →	high‑affinity gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,546,932	0	T	C	100.0%	105.1 / NA	31	V125A (GTG→GCG)	gntT	high‑affinity gluconate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/18);  total (13/18)

ACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACC  >  NC_000913/3546817‑3547053                                                                                                                    |                                                                                                                          acGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGaa                                                                                                   <  1:440608/140‑1 (MQ=255) acGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGaa                                                                                                   <  2:699007/140‑1 (MQ=255)  cGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAat                                                                                                  >  1:491008/1‑140 (MQ=255)       aTTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTcc                                                                                             >  2:682735/1‑140 (MQ=255)       aTTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTcc                                                                                             >  1:46471/1‑140 (MQ=255)       aTTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTcc                                                                                             >  1:525591/1‑140 (MQ=255)        ttGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCa                                                                                            <  1:478816/140‑1 (MQ=255)        ttGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCa                                                                                            <  1:533380/140‑1 (MQ=255)        ttGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCa                                                                                            <  1:185011/140‑1 (MQ=255)                 ttGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTAt                                                                                   >  1:227490/1‑140 (MQ=255)                   ggTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGt                                                                                 <  2:227490/140‑1 (MQ=255)                     tAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGTCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTg                                                                               <  2:634799/140‑1 (MQ=255)                       aaaaaCACCTCCAGTGGGCGGTGGTTCTTACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGgt                                                                             <  1:678476/140‑1 (MQ=255)                                         cGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCa                                                           <  1:682735/140‑1 (MQ=255)                                          ggtggtACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCAc                                                          <  2:46471/140‑1 (MQ=255)                                          ggtggtACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCAc                                                          <  2:491008/140‑1 (MQ=255)                                                      cGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATggcgg                                                                >  2:471667/1‑122 (MQ=255)                                                      cGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATggcgg                                                                <  1:471667/122‑1 (MQ=255)                                                                   ggTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGcc                                 >  1:505825/1‑140 (MQ=255)                                                                               ttCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCggg                     <  2:483525/140‑1 (MQ=255)                                                                                    tGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGa                >  2:490055/1‑140 (MQ=255)                                                                                         tGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGa                >  1:576432/1‑135 (MQ=255)                                                                                         tGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGa                <  2:576432/135‑1 (MQ=255)                                                                                         tGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg           >  1:584964/1‑140 (MQ=255)                                                                                         tGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg           >  1:93478/1‑140 (MQ=255)                                                                                         tGGGCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg           >  2:371584/1‑140 (MQ=255)                                                                                           ggCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTg             >  2:209525/1‑136 (MQ=255)                                                                                           ggCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTg             <  1:209525/136‑1 (MQ=255)                                                                                           ggCTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGAt         >  1:73735/1‑140 (MQ=255)                                                                                             cTTTGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTg       <  2:73735/140‑1 (MQ=255)                                                                                               ttGTGCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGcc     <  1:473249/140‑1 (MQ=255)                                                                                                  tgCTGATGCTGCCGCTGGCGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGccacc  <  2:93478/140‑1 (MQ=255)                                                                                                                    |                                                                                                                          ACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACC  >  NC_000913/3546817‑3547053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑