Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	284,312	A→G	H371R (CAC→CGC)	yagF →	CP4‑6 prophage; D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	284,312	0	A	G	100.0%	154.6 / NA	45	H371R (CAC→CGC)	yagF	CP4‑6 prophage; D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/19);  total (26/19)

ATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCG  >  NC_000913/284179‑284446                                                                                                                                      |                                                                                                                                       aTCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTgcg                                                                                                                                  <  1:212530/140‑1 (MQ=255)          gTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCgg                                                                                                                         <  2:712673/140‑1 (MQ=255)            tGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTgcg                                                                                                                                  >  1:681221/1‑129 (MQ=255)            tGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTgcg                                                                                                                                  <  2:681221/129‑1 (MQ=255)            tGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGcc                                                                                                                       >  2:450296/1‑140 (MQ=255)            tGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGcc                                                                                                                       >  2:235435/1‑140 (MQ=255)            tGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGcc                                                                                                                       >  1:711130/1‑140 (MQ=255)              aGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCctg                                                                                                                     <  2:511533/140‑1 (MQ=255)              aGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCctg                                                                                                                     >  2:409421/1‑140 (MQ=255)              aGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCctg                                                                                                                     >  1:572652/1‑140 (MQ=255)                  cTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCtgctgc                                                                                                                 >  1:585020/1‑140 (MQ=255)                       cgcgCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTg                                                                                                            <  1:312579/140‑1 (MQ=255)                              cAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCa                                                                                                     <  2:162893/140‑1 (MQ=255)                               aaCCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCAt                                                                                                    >  1:415840/1‑140 (MQ=255)                                    tAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTg                                                                                                            <  2:550199/127‑1 (MQ=255)                                    tAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTg                                                                                                            >  1:550199/1‑127 (MQ=255)                                    tAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCAtgaccg                                                                                               >  2:184098/1‑140 (MQ=255)                                        gTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCAtgaccgtgac                                                                                           <  2:572652/140‑1 (MQ=255)                                           ccGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCgg                                                                                        >  1:605212/1‑140 (MQ=255)                                           ccGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCgg                                                                                        >  2:400206/1‑140 (MQ=255)                                                   ggTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACgg                                                                                >  1:193299/1‑140 (MQ=255)                                                         cGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCg                                                                          <  1:235435/140‑1 (MQ=255)                                                         cGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCg                                                                          >  1:312931/1‑140 (MQ=255)                                                         cGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCg                                                                          >  1:547033/1‑140 (MQ=255)                                                          gTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCga                                                                         <  2:193299/140‑1 (MQ=255)                                                          gTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCga                                                                         <  2:415840/140‑1 (MQ=255)                                                           tgctgcCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCgag                                                                        <  2:360791/140‑1 (MQ=255)                                                           tgctgcCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCgag                                                                        <  2:439979/140‑1 (MQ=255)                                                           tgctgcCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCAGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCgag                                                                        >  2:354519/1‑140 (MQ=255)                                                                     cGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAAt                                                              >  2:690967/1‑140 (MQ=255)                                                                     cGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAAt                                                              <  1:381523/140‑1 (MQ=255)                                                                                  cACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTc                                                 >  1:414874/1‑140 (MQ=255)                                                                                        aCCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAgcg                                           >  1:74455/1‑140 (MQ=255)                                                                                          cGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGcc                                         <  2:74455/140‑1 (MQ=255)                                                                                           gTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCg                                        >  1:319448/1‑140 (MQ=255)                                                                                            tGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCgg                                       >  2:454054/1‑140 (MQ=255)                                                                                            tGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCgg                                       >  2:618408/1‑140 (MQ=255)                                                                                            tGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCgg                                       >  1:66348/1‑140 (MQ=255)                                                                                              cgcgcCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGgc                                     >  2:223291/1‑140 (MQ=255)                                                                                                   cTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCt                                >  1:621163/1‑140 (MQ=255)                                                                                                        tCGCGGGCGGCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGc                           <  1:450296/140‑1 (MQ=255)                                                                                                          gcgggcggCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCa                         <  2:585020/140‑1 (MQ=255)                                                                                                          gcgggcggCGTGCCGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCa                         <  2:696397/140‑1 (MQ=255)                                                                                                                      ccGGAGGTGATGCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGa             >  2:470562/1‑140 (MQ=255)                                                                                                                                 gCTCCGCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCg  <  1:470562/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       ATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCG  >  NC_000913/284179‑284446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑