Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,201,660	A→G	V10A (GTA→GCA)	zraP ←	zinc responsive, periplasmic protein with chaperone activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,201,660	0	A	G	100.0%	169.1 / NA	48	V10A (GTA→GCA)	zraP	zinc responsive, periplasmic protein with chaperone activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/28);  total (20/28)

AAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAA  >  NC_000913/4201524‑4201790                                                                                                                                         |                                                                                                                                   aaaGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCa                                                                                                                                 <  2:327721/140‑1 (MQ=255)            ggATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTc                                                                                                                      <  2:242150/140‑1 (MQ=255)                 ttCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGACATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGtt                                                                                                                 <  1:587994/140‑1 (MQ=255)                       cACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGttt                                                                                                           <  1:625406/140‑1 (MQ=255)                        aCGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTc                                                                                                          <  1:20048/140‑1 (MQ=255)                           ctgtctgtTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATg                                                                                                       <  2:557031/140‑1 (MQ=255)                             gtctgtTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGt                                                                                                     <  2:17947/140‑1 (MQ=255)                              tctgtTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGtt                                                                                                    >  2:184686/1‑140 (MQ=255)                              tctgtTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGtt                                                                                                    >  1:353222/1‑140 (MQ=255)                               ctgtTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTa                                                                                                   <  2:37810/140‑1 (MQ=255)                                tgttgtTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTaa                                                                                                  <  1:125775/140‑1 (MQ=255)                                tgttgtTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTaa                                                                                                  <  2:353222/140‑1 (MQ=255)                                tgttgtTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTaa                                                                                                  <  2:66741/140‑1 (MQ=255)                                 gttgttCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAAt                                                                                                 <  2:327400/140‑1 (MQ=255)                                    gttCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCAATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAAtcct                                                                                              >  2:603995/1‑140 (MQ=255)                                        gCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAg                                                                                          >  2:146172/1‑140 (MQ=255)                                        gCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAg                                                                                          >  1:705692/1‑140 (MQ=255)                                           ggTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTgg                                                                                       <  1:146172/140‑1 (MQ=255)                                            gTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGt                                                                                      <  1:248586/140‑1 (MQ=255)                                            gTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGt                                                                                      >  1:159130/1‑140 (MQ=255)                                                      cgGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACtt                                                                            >  1:52467/1‑140 (MQ=255)                                                       ggCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCAATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTc                                                                           <  1:603995/140‑1 (MQ=255)                                                         cATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCgg                                                                         <  1:377427/140‑1 (MQ=255)                                                           ttttGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGt                                                                       <  2:10751/140‑1 (MQ=255)                                                           ttttGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGt                                                                       <  2:159130/140‑1 (MQ=255)                                                                      caTACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTc                                                            >  2:659659/1‑140 (MQ=255)                                                                        tACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCg                                                          >  2:613464/1‑140 (MQ=255)                                                                            gtgtCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGcc                                                      <  1:613464/140‑1 (MQ=255)                                                                             tgtCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGc                                                       >  2:167369/1‑138 (MQ=255)                                                                             tgtCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGc                                                       <  1:167369/138‑1 (MQ=255)                                                                             tgtCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCa                                                     >  2:630807/1‑140 (MQ=255)                                                                             tgtCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCa                                                     >  1:176259/1‑140 (MQ=255)                                                                             tgtCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCa                                                     >  2:287213/1‑140 (MQ=255)                                                                               tCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGCTTGTCTCACTTCGGGTATTGCATCTTCCGTGCCAAc                                                   >  1:585447/1‑140 (MQ=255)                                                                                 cgccgTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGa                                                 >  2:297481/1‑140 (MQ=255)                                                                                    cgTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGa                                              >  1:47606/1‑140 (MQ=255)                                                                                     gTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGaa                                             <  2:176259/140‑1 (MQ=255)                                                                                      tGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGaaa                                            <  1:184686/140‑1 (MQ=255)                                                                                      tGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGaaa                                            <  2:22646/140‑1 (MQ=255)                                                                                                 aGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGa                                 <  1:297481/140‑1 (MQ=255)                                                                                                         aTCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAAt                         >  1:670600/1‑140 (MQ=255)                                                                                                         aTCCCATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAAt                         <  2:670600/140‑1 (MQ=255)                                                                                                             cATCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTgg                     <  1:287213/140‑1 (MQ=255)                                                                                                               tCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCa                   <  2:47606/140‑1 (MQ=255)                                                                                                               tCGCCATTGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCa                   <  1:630807/140‑1 (MQ=255)                                                                                                                     ttGCTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATaa             >  1:592886/1‑140 (MQ=255)                                                                                                                        cTGAAAGCGCCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATaaagg          >  2:674178/1‑138 (MQ=255)                                                                                                                                gcCATCATTGCCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTaaaaa  >  2:536242/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   AAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAA  >  NC_000913/4201524‑4201790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑