Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,305,163	A→G	S479P (TCG→CCG)	yjcS ←	alkyl sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,305,163	0	A	G	100.0%	200.5 / NA	57	S479P (TCG→CCG)	yjcS	alkyl sulfatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/27);  total (30/27)

CCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTA  >  NC_000913/4305030‑4305298                                                                                                                                      |                                                                                                                                        ccGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCg                                                                                                                                   >  2:401098/1‑140 (MQ=255)   ggTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGt                                                                                                                                 <  2:70957/140‑1 (MQ=255)         gTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCAc                                                                                                                           <  1:716544/140‑1 (MQ=255)               aCCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACcttgct                                                                                                                     >  2:489414/1‑140 (MQ=255)                   cgcCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGtt                                                                                                                 >  2:614478/1‑140 (MQ=255)                   cgcCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGtt                                                                                                                 >  1:550768/1‑140 (MQ=255)                   cgcCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGtt                                                                                                                 >  1:453588/1‑140 (MQ=255)                   cgcCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGtt                                                                                                                 >  2:390497/1‑140 (MQ=255)                    gcCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTc                                                                                                                >  1:378501/1‑140 (MQ=255)                     ccATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCg                                                                                                               <  1:401098/140‑1 (MQ=255)                       aTGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGct                                                                                                             >  2:188763/1‑140 (MQ=255)                             cGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTgc                                                                                                       >  2:555073/1‑140 (MQ=255)                               gACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTgcgc                                                                                                     <  2:136170/140‑1 (MQ=255)                                 cTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTgcg                                                                                                      <  2:696438/137‑1 (MQ=255)                                 cTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTgcg                                                                                                      >  1:696438/1‑137 (MQ=255)                                    ggCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGt                                                                                                >  1:390582/1‑140 (MQ=255)                                    ggCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGt                                                                                                >  2:43111/1‑140 (MQ=255)                                      ccTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtg                                                                                              >  2:356647/1‑140 (MQ=255)                                                cAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACggg                                                                                    >  1:536959/1‑140 (MQ=255)                                                cAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACggg                                                                                    <  1:356647/140‑1 (MQ=255)                                                      ttCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAAc                                                                              <  1:614478/140‑1 (MQ=255)                                                        cAAAGTTATTCGCTTGCAGATTCTTCTCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCg                                                                            >  1:58168/1‑140 (MQ=255)                                                               aTTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAgcg                                                                     >  2:606588/1‑140 (MQ=255)                                                                ttCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAgcgc                                                                    >  1:650243/1‑140 (MQ=255)                                                                ttCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAgcgc                                                                    >  2:393431/1‑140 (MQ=255)                                                                   gCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTg                                                                 <  2:390582/140‑1 (MQ=255)                                                                       gCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACg                                                             >  2:701466/1‑140 (MQ=255)                                                                       gCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACg                                                             <  2:536959/140‑1 (MQ=255)                                                                       gCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACg                                                             <  1:426843/140‑1 (MQ=255)                                                                              cttcGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt                                                      <  2:384897/140‑1 (MQ=255)                                                                                 cgcgACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTAc                                                   <  1:606588/140‑1 (MQ=255)                                                                                 cgcgACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTAc                                                   <  2:338444/140‑1 (MQ=255)                                                                                     aCCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCAt                                               <  1:43111/140‑1 (MQ=255)                                                                                       cTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCAtct                                             >  2:599328/1‑140 (MQ=255)                                                                                           tCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCAc                                         <  1:555073/140‑1 (MQ=255)                                                                                            cACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCAcc                                        <  1:674524/140‑1 (MQ=255)                                                                                                cGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCAt                                               >  2:559305/1‑129 (MQ=255)                                                                                                cGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCAt                                               <  1:559305/129‑1 (MQ=255)                                                                                                cGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGAc                                    >  2:349455/1‑140 (MQ=255)                                                                                                    ttGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATa                                >  2:276634/1‑140 (MQ=255)                                                                                                         ggcAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAt                           >  1:203331/1‑140 (MQ=255)                                                                                                          gcAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATg                          <  1:599328/140‑1 (MQ=255)                                                                                                          gcAATCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATg                          <  2:650243/140‑1 (MQ=255)                                                                                                             aTCACCTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAg                       >  1:156571/1‑140 (MQ=255)                                                                                                                 ccTGTTTCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTa                   <  2:550768/140‑1 (MQ=255)                                                                                                                       tCAGCAGTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCggg             >  2:677606/1‑140 (MQ=255)                                                                                                                        cagcagTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGt            <  2:390933/140‑1 (MQ=255)                                                                                                                          gcagTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTa          >  1:571701/1‑140 (MQ=255)                                                                                                                           cagTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTAc         <  2:453588/140‑1 (MQ=255)                                                                                                                           cagTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTAACCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTAc         >  2:680802/1‑140 (MQ=255)                                                                                                                             gTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACcg       <  2:304484/140‑1 (MQ=255)                                                                                                                             gTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACcg       <  2:58168/140‑1 (MQ=255)                                                                                                                             gTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACcg       <  1:585269/140‑1 (MQ=255)                                                                                                                             gTTCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACcg       >  1:304484/1‑140 (MQ=255)                                                                                                                              ttCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACcgt      <  2:378501/140‑1 (MQ=255)                                                                                                                               tCTGCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACcgtc     >  1:253814/1‑140 (MQ=255)                                                                                                                                  gCCGGCCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTa  <  2:571701/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        CCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTA  >  NC_000913/4305030‑4305298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑