Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,531,749	A→G	intergenic (‑98/+688)	sgcX ← / ← yjhP	KpLE2 phage‑like element; putative endoglucanase with Zn‑dependent exopeptidase domain/KpLE2 phage‑like element; putative methyltransferase YjhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,531,749	0	A	G	100.0%	127.1 / NA	37	intergenic (‑98/+688)	sgcX/yjhP	KpLE2 phage‑like element; putative endoglucanase with Zn‑dependent exopeptidase domain/KpLE2 phage‑like element; putative methyltransferase YjhP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/17);  total (20/17)

AGCGAGAACAGGGTTTCCTGCACAGAAAATGACATAGCTATCTCCGTAGACCGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAAGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGGAGAGACCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAAC  >  NC_000913/4531617‑4531872                                                                                                                                     |                                                                                                                            aGCGAGAACAGGGTTTCCTGCACAGAAAATGACATAGCTATCTCCGTAGACCGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAAc                                                                                                                      <  2:731902/140‑1 (MQ=255)                    gCACAGAAAATGACATAGCTATCTCCGTAGACCGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAg                                                                                                   >  2:657329/1‑140 (MQ=255)                    gCACAGAAAATGACATAGCTATCTCCGTAGACCGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAg                                                                                                   <  1:573451/140‑1 (MQ=255)                    gCACAGAAAATGACATAGCTATCTCCGTAGACAGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAg                                                                                                   <  2:138126/140‑1 (MQ=255)                      acaGAAAATGACATAGCTATCTCCGTAGACCGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGcc                                                                                                 >  1:447053/1‑140 (MQ=255)                      acaGAAAATGACATAGCTATCTCCGTAGACCGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGcc                                                                                                 >  1:681998/1‑140 (MQ=255)                         gAAAATGACATAGCTATCTCCGTAGACCGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAg                                                                                              <  1:314478/140‑1 (MQ=255)                          aaaaTGACATAGCTATCTCCGTAGACCGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAg                                                                                              <  2:482064/139‑1 (MQ=255)                          aaaaTGACATAGCTATCTCCGTAGACCGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAg                                                                                              >  1:482064/1‑139 (MQ=255)                          aaaaTGACATAGCTATCTCCGTAGACCGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGt                                                                                             >  2:369180/1‑140 (MQ=255)                                    aGCTATCTCCGTAGACCGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAg                                                                                   >  1:489973/1‑140 (MQ=255)                                      cTATCTCCGTAGACCGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAgcg                                                                                 >  1:24375/1‑140 (MQ=255)                                           tcCGTAGACCGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCaa                                                                            >  1:475618/1‑140 (MQ=255)                                               tAGACCGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGtt                                                                        >  1:638868/1‑140 (MQ=255)                                               tAGACCGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGtt                                                                        >  2:453303/1‑140 (MQ=255)                                                     gTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGaa                                                                  >  1:2376/1‑140 (MQ=255)                                                       aaaaCAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAg                                                                >  1:157754/1‑140 (MQ=255)                                                       aaaaCAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGCGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAg                                                                >  1:491419/1‑140 (MQ=255)                                                           cAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCa                                                                             >  1:517787/1‑123 (MQ=255)                                                           cAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCa                                                                             <  2:517787/123‑1 (MQ=255)                                                              tCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGAGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACggg                                                         <  2:638868/140‑1 (MQ=255)                                                                   cGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGga                                                    <  1:369180/140‑1 (MQ=255)                                                                    gTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGgag                                                   >  2:426591/1‑140 (MQ=255)                                                                          cGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGGAGAGACCa                                             <  1:426591/140‑1 (MQ=255)                                                                           gTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCCCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGGAGAGACCAg                                            <  1:457925/140‑1 (MQ=255)                                                                                       cAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGGAGAGACCAGTGATTATGGTTg                                <  1:96493/140‑1 (MQ=255)                                                                                       cAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGGAGAGACCAGTGATTATGGTTg                                >  2:708730/1‑140 (MQ=255)                                                                                       cAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGGAGAGACCAGTGATTATGGTTg                                >  2:647893/1‑140 (MQ=255)                                                                                         gACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGGAGAGACCAGTGATTATGGTTGaa                              <  1:453303/140‑1 (MQ=255)                                                                                             ggTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGGAGAGACCAGTGATTATGGTTGAaataa                          <  1:708730/140‑1 (MQ=255)                                                                                              gTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGGAGAGACCAGTGATTATGGTTGAaataat                         >  1:243054/1‑140 (MQ=255)                                                                                               tCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGGAGAGACCAGTGATTATGGTTGAAATAATg                        <  2:2376/140‑1 (MQ=255)                                                                                                  aataatTGTGAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGGAGAGACCAGTGATTATGGTTGAAATAATGAgg                     <  1:159122/140‑1 (MQ=255)                                                                                                        tgtgAGAACGTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGGAGAGACCAGTGATTATGGTTGAAATAATGAGGATGAAt               >  2:476187/1‑140 (MQ=255)                                                                                                                 gTTCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGGAGAGACCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCgg      >  1:535736/1‑140 (MQ=255)                                                                                                                   tCACGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGGAGAGACCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCAGTa    <  2:157754/140‑1 (MQ=255)                                                                                                                     aCGTGAAAACGCGCAAGGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGGAGAGACCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAAc  <  2:24375/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                            AGCGAGAACAGGGTTTCCTGCACAGAAAATGACATAGCTATCTCCGTAGACCGTAAAACAATCACTCGTTGAGCGTTTTATAGGCGCAGACTGGTCGAATAATTGTGAGAACGTTCACGTGAAAACGCGCAAAGCTGAACAAATAAAAAATTATGTGAGCCGAGTGGAATAAAAGCGCTCAACGTTTGTGAAAGTTACGGGCTGGAGAGACCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAAC  >  NC_000913/4531617‑4531872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑