Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,566,166	A→G	F361L (TTT→CTT)	yjiN ←	DUF445 domain‑containing protein YjiN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,566,166	0	A	G	100.0%	122.4 / NA	36	F361L (TTT→CTT)	yjiN	DUF445 domain‑containing protein YjiN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/18);  total (18/18)

CCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAACTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCGATGCGTTCTTTCACACGAGAATCTTCA  >  NC_000913/4566033‑4566299                                                                                                                                      |                                                                                                                                      ccGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAgg                                                                                                                                 >  2:223876/1‑140 (MQ=255)   gCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGgcg                                                                                                                               >  2:85670/1‑140 (MQ=255)      aaCCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGa                                                                                                                            >  2:255983/1‑140 (MQ=255)      aaCCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGa                                                                                                                            >  2:522497/1‑140 (MQ=255)      aaCCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGa                                                                                                                            >  1:588228/1‑140 (MQ=255)        ccAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGAcg                                                                                                                          <  2:588228/140‑1 (MQ=255)               aCCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTgcg                                                                                                                   <  1:723108/140‑1 (MQ=255)                 cGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGcgcg                                                                                                                 >  2:331688/1‑140 (MQ=255)                   ttGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGc                                                                                                               <  2:142582/140‑1 (MQ=255)                   ttGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGc                                                                                                               <  1:678615/140‑1 (MQ=255)                           aaTAAACTGCAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCaa                                                                                                       >  2:298240/1‑140 (MQ=255)                              aaaCTGCAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGAt                                                                                                    <  1:20888/140‑1 (MQ=255)                              aaaCTGCAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGAt                                                                                                    <  1:255983/140‑1 (MQ=255)                              aaaCTGCAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGAt                                                                                                    <  1:522497/140‑1 (MQ=255)                                    cAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCAt                                                                                              <  1:456868/140‑1 (MQ=255)                                           ttGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAacga                                                                                       <  1:14964/140‑1 (MQ=255)                                                   atTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCa                                                                               >  2:96335/1‑140 (MQ=255)                                                            tATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGcatcat                                                                      >  2:76304/1‑140 (MQ=255)                                                                                gcgcATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAGTTAACGTTTCGGCa                                                  >  2:446314/1‑140 (MQ=255)                                                                                    aTCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAAcc                                              >  2:188737/1‑140 (MQ=255)                                                                                     tCCCAGCTTTTTACCGTATCGCTGATGTGACGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCa                                             >  2:664038/1‑140 (MQ=255)                                                                                       ccAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCAtt                                           >  1:218197/1‑140 (MQ=255)                                                                                        cAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTg                                          >  1:156559/1‑140 (MQ=255)                                                                                                     tATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCg                             >  2:398195/1‑140 (MQ=255)                                                                                                     tATCGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCg                             >  2:556752/1‑140 (MQ=255)                                                                                                        cGCTGATGTGGCGCGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCGATg                          <  1:193196/140‑1 (MQ=255)                                                                                                                  gcgcGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCGATGCGTTCTTTca                >  2:134896/1‑140 (MQ=255)                                                                                                                  gcgcGTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCGATGCGTTCTTTca                >  2:594903/1‑140 (MQ=255)                                                                                                                    gcgTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCGATGCGTTCTTTcaca              <  1:93581/140‑1 (MQ=255)                                                                                                                    gcgTCAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCGATGCGTTCTTTcaca              <  1:76304/140‑1 (MQ=255)                                                                                                                      gccAGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCGATGCGTTCTTTCACACg            <  1:638157/138‑1 (MQ=255)                                                                                                                         aGGAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCGATGCGTTCTTTCACACgaga         <  1:223876/140‑1 (MQ=255)                                                                                                                           gAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCGATGCGTTCTTTCACACGAGAAt       <  1:398195/140‑1 (MQ=255)                                                                                                                           gAATGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCGATGCGTTCTTTCACACGAGAAt       <  1:188737/140‑1 (MQ=255)                                                                                                                              tGCGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCGATGCGTTCTTTCACACGAGAATCtt    <  1:331688/140‑1 (MQ=255)                                                                                                                                cGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCGATGCGTTCTTTCACACGAGAATCTTCa  <  2:156559/140‑1 (MQ=255)                                                                                                                                cGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCGATGCGTTCTTTCACACGAGAATCTTCa  >  1:62813/1‑140 (MQ=255)                                                                                                                                cGGAAAGCTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCGATGCGTTCTTTCACACGAGAATCTTCa  <  1:298240/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      CCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTGCCGCGACATATCCCGCGCATCCCAGCTTTTTACCGTATCGCTGATGTGGCGCGTCAGGAATGCGGAAAACTCAGGCGCGACGCGGTGCGCGGCTTGTTCAAGATGACCATTTAACGACGCCCGCAAGGCATCATCGGCAATTAACGTTTCGCCAAACCATTGACCCGCTCGTGCGATGCGTTCTTTCACACGAGAATCTTCA  >  NC_000913/4566033‑4566299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑