Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	512,765	T→C	Y64H (TAT→CAT)	ybaT →	putative transporter YbaT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	512,765	0	T	C	100.0%	72.6 / NA	22	Y64H (TAT→CAT)	ybaT	putative transporter YbaT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/10);  total (12/10)

CGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGCTCTCGTTACTGTACCTGTTGACGCTGGCGGTGAGCATCGCC  >  NC_000913/512629‑512896                                                                                                                                         |                                                                                                                                    cgtTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATg                                                                                                                                  >  1:466331/1‑140 (MQ=255) cgtTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATg                                                                                                                                  >  2:275690/1‑140 (MQ=255)             cATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATGCGCGTCTggggg                                                                                                                      >  1:390126/1‑140 (MQ=255)                 ggggCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATGCGCGTCTGGGGGCGAg                                                                                                                  <  1:320695/140‑1 (MQ=255)                  gggCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATGCGCGTCTGGGGGCGAGc                                                                                                                 <  2:22434/140‑1 (MQ=255)                                             cgcTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGAc                                                                                      >  1:162550/1‑140 (MQ=255)                                             cgcTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGAc                                                                                      >  1:333514/1‑140 (MQ=255)                                                       gCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTtcgtc                                                                            <  1:275690/140‑1 (MQ=255)                                                        cAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTtcgtcg                                                                           <  2:390126/140‑1 (MQ=255)                                                           gCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTGTGGCGGTATTGTGGCGATATTTTCCGGTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCgg                                                                        >  2:649837/1‑140 (MQ=255)                                                                      aaTGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACg                                                             >  1:132183/1‑140 (MQ=255)                                                                              ccTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCttt                                                     >  1:80431/1‑140 (MQ=255)                                                                              ccTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCttt                                                     >  1:114501/1‑140 (MQ=255)                                                                               cTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCtttt                                                    >  1:448551/1‑140 (MQ=255)                                                                                         gTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGct                                          <  2:536240/140‑1 (MQ=255)                                                                                              cTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGCTCTCGt                                     >  2:456063/1‑140 (MQ=255)                                                                                               tttGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGCTCTCGtt                                    <  2:162550/140‑1 (MQ=255)                                                                                               tttGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGCTCTCGtt                                    <  2:333514/140‑1 (MQ=255)                                                                                                                          ttttCCGGTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGCTCTCGTTACTGTACCTGTTGACGCTg                 <  1:708877/132‑1 (MQ=255)                                                                                                                          ttttCCGGTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGCTCTCGTTACTGTACCTGTTGACGCTg                 >  2:708877/1‑132 (MQ=255)                                                                                                                           tttCCGGTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGCTCTCGTTACTGTACCTGTTGACGCTGGCGGTGAGc        <  2:80431/140‑1 (MQ=255)                                                                                                                                 gTTATGCCCATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGCTCTCGTTACTGTACCTGTTGACGCTGGCGGTGAGCATCGcc  <  1:660945/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGCTCTCGTTACTGTACCTGTTGACGCTGGCGGTGAGCATCGCC  >  NC_000913/512629‑512896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑