Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	566,570	A→G	intergenic (‑209/‑114)	exoD ← / → renD	DLP12 prophage; putative exonuclease ExoD/DLP12 prophage; protein RenD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	566,570	0	A	G	100.0%	146.7 / NA	42	intergenic (‑209/‑114)	exoD/renD	DLP12 prophage; putative exonuclease ExoD/DLP12 prophage; protein RenD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/26);  total (16/26)

AACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAAT  >  NC_000913/566436‑566703                                                                                                                                       |                                                                                                                                      aaCGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGa                                                                                                                                  <  2:182089/140‑1 (MQ=255)   cgcgCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAAc                                                                                                                                <  1:1520/140‑1 (MQ=255)   cgcgCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAAc                                                                                                                                <  2:401190/140‑1 (MQ=255)    gcgcATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAAcc                                                                                                                               <  1:200257/140‑1 (MQ=255)        aTTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTa                                                                                                                           <  2:665370/140‑1 (MQ=255)          tACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTaaa                                                                                                                         <  1:230596/140‑1 (MQ=255)                    ttACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGTATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCt                                                                                                               >  1:439456/1‑140 (MQ=255)                    ttACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAaac                                                                                                                       <  2:728863/132‑1 (MQ=255)                    ttACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAaac                                                                                                                       >  1:728863/1‑132 (MQ=255)                     tACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTg                                                                                                              <  1:680097/140‑1 (MQ=255)                          aCTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGTATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATg                                                                                                         <  2:439456/140‑1 (MQ=255)                              gTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCg                                                                                                     >  2:673149/1‑140 (MQ=255)                                 ccAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTa                                                                                                  >  1:571250/1‑140 (MQ=255)                                 ccAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTa                                                                                                  <  2:613634/140‑1 (MQ=255)                                 ccAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTa                                                                                                  <  1:673149/140‑1 (MQ=255)                                 ccAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTa                                                                                                  <  1:409476/140‑1 (MQ=255)                                 ccAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTa                                                                                                  <  2:536354/140‑1 (MQ=255)                                    gAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTa                                                                                                  >  2:429298/1‑137 (MQ=255)                                    gAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTa                                                                                                  <  1:429298/137‑1 (MQ=255)                                     aaCATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGAcc                                                                                              >  2:320173/1‑140 (MQ=255)                                         tGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTa                                                                                          >  2:693498/1‑140 (MQ=255)                                          gCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTaa                                                                                         >  2:111411/1‑140 (MQ=255)                                          gCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTaa                                                                                         >  2:588128/1‑140 (MQ=255)                                              gCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCg                                                                                     >  2:99748/1‑140 (MQ=255)                                              gCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCg                                                                                     <  1:320173/140‑1 (MQ=255)                                                        tggctgACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGct                                                                           <  2:571250/140‑1 (MQ=255)                                                                            cgcAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCa                                                            <  2:253850/135‑1 (MQ=255)                                                                            cgcAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCa                                                            >  1:253850/1‑135 (MQ=255)                                                                             gcAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAAtt                                                      <  1:693498/140‑1 (MQ=255)                                                                              cAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTa                                                     >  2:622838/1‑140 (MQ=255)                                                                                    tGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTa                                               >  2:522031/1‑140 (MQ=255)                                                                                                    tGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAgg                               <  1:99748/140‑1 (MQ=255)                                                                                                    tGTATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAgg                               <  1:622838/140‑1 (MQ=255)                                                                                                      tATGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAg                             <  1:95727/140‑1 (MQ=255)                                                                                                       aTGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGc                            <  1:588128/140‑1 (MQ=255)                                                                                                       aTGACAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGc                            <  1:111411/140‑1 (MQ=255)                                                                                                           cAGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGCAAgt                        >  2:429690/1‑140 (MQ=255)                                                                                                            aGCGCGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGCAAgtg                       >  1:709514/1‑140 (MQ=255)                                                                                                             gcgcGAATTAACCGTGGTGAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGCAAgtgt                      >  1:131359/1‑140 (MQ=255)                                                                                                                             gtgAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAgg      <  2:709514/140‑1 (MQ=255)                                                                                                                             gtgAGACGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAgg      <  2:466061/140‑1 (MQ=255)                                                                                                                                 gaCGATGCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAat  <  2:131359/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      AACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAAT  >  NC_000913/566436‑566703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑