Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	642,281	A→G	E65G (GAA→GGA)	ybdR →	putative Zn(2(+))‑dependent alcohol dehydrogenase YbdR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	642,281	0	A	G	100.0%	95.6 / NA	28	E65G (GAA→GGA)	ybdR	putative Zn(2(+))‑dependent alcohol dehydrogenase YbdR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/15);  total (13/15)

AGGCAGATGATATTATTCTGCGTATTACGGCAACGGCGATCTGTGGCTCTGACCTCCATCTTTATCGAGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGAAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTGCAACAATATGCCGCCTGCGAAAATACCAATG  >  NC_000913/642158‑642412                                                                                                                            |                                                                                                                                    aGGCAGATGATATTATTCTGCGTATTACGGCAACGGCGATCTGTGGCTCTGACC‑CCATCTTTATCGAGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGaa                                                                                                                    >  1:312285/1‑140 (MQ=255)        tgatATTATTCTGCGTATTACGGCAACGGCGATCTGTGGCTCTGACCTCCATCTTTATCGAGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACg                                                                                                              >  2:35319/1‑140 (MQ=255)         gacattattCTGCGCATTACGGCAACGCCGAACTGTGGCTCTGACCTCCATCTTTATCGAGGCAAAATACCTCCGGTTAAACCTGGCGATATTTTTGGTCATCAATTTATGGGGAGAGTAGTTGCAACCGGAAAGGACGt                                                                                                             >  2:211020/4‑140 (MQ=255)             ttattCTGCGTATTACGGCAACGGCGATCTGTGGCTCTGACCTCCATCTTTATCGAGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTaaaa                                                                                                         >  2:578986/1‑140 (MQ=255)                 tCTGCGTATTACGGCAACGGCGATCTGTGGCTCTGACCTCCATCTTTATCGAGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAAtt                                                                                                     >  2:595001/1‑140 (MQ=255)                            cGGCAACGGCGATCTGTGGCTCTGACCTCCATCTTTATCGAGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGc                                                                                          <  2:723619/140‑1 (MQ=255)                               cAACGGCGATCTGTGGCTCTGACCTCCATCTTTATCGAGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGAc                                                                                       <  1:578986/140‑1 (MQ=255)                                 aCGGCGATCTGTGGCTCTGACCTCCATCTTTATCGAGGGAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCg                                                                                     <  1:44539/140‑1 (MQ=255)                                 aCGGCGATCTGTGGCTCTGACCTCCATCTTTATCGAGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCg                                                                                     <  1:359395/140‑1 (MQ=255)                                              gCTCTGACCTCCATCTTTATCGAGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCg                                                                        <  1:595001/140‑1 (MQ=255)                                                    aCCTCCATCTTTATCGAGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTc                                                                  <  1:318080/140‑1 (MQ=255)                                                    aCCTCCATCTTTATCGAGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTc                                                                  >  1:373048/1‑140 (MQ=255)                                                      cTCCATCTTTATCGAGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCAt                                                                <  2:441291/140‑1 (MQ=255)                                                         cATCTTTATCGAGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGc                                                             >  2:212519/1‑140 (MQ=255)                                                         cATCTTTATCGAGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGc                                                             >  2:356337/1‑140 (MQ=255)                                                                   gAGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGAc                                                   <  1:35319/140‑1 (MQ=255)                                                                    aGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACt                                                  <  2:373048/140‑1 (MQ=255)                                                                        aaaaTACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGttt                                              <  1:212519/140‑1 (MQ=255)                                                                               cTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGt                                       >  2:230107/1‑140 (MQ=255)                                                                                tCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTc                                      <  1:230107/140‑1 (MQ=255)                                                                                     ttAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTg                                 <  2:49787/140‑1 (MQ=255)                                                                                              gCGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTGCAACAatat                        >  1:421282/1‑140 (MQ=255)                                                                                               cGATATTTTTGGTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTGCAACAATATg                       >  2:92476/1‑140 (MQ=255)                                                                                                         ggTCATGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTGCAACAATATGCCGCCTGCGa             >  1:623402/1‑140 (MQ=255)                                                                                                              tGAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTGCAACAATATGCCGCCTGCGAAAATa        >  2:187055/1‑140 (MQ=255)                                                                                                               gAATTTATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTGCAACAATATGCCGCCTGCGAAAATAc       >  1:127913/1‑140 (MQ=255)                                                                                                                   ttATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTGCAACAATATGCCGCCTGCGAAAATACCAAt   <  2:421282/140‑1 (MQ=255)                                                                                                                   ttATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTGCAACAATATGCCGCCTGCGAAAATACCAAt   <  1:716635/140‑1 (MQ=255)                                                                                                                    tATGGGGGGAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTGCAACAATATGCCGCCTGCGAAAATACCAATg  <  1:92476/140‑1 (MQ=255)                                                                                                                            |                                                                                                                                    AGGCAGATGATATTATTCTGCGTATTACGGCAACGGCGATCTGTGGCTCTGACCTCCATCTTTATCGAGGCAAAATACCTCAGGTTAAACATGGCGATATTTTTGGTCATGAATTTATGGGGGAAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTGCAACAATATGCCGCCTGCGAAAATACCAATG  >  NC_000913/642158‑642412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑