Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	44,304	C→A	A42D (GCC→GAC)	fixC →	putative oxidoreductase FixC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	44,304	0	C	A	100.0%	117.8 / NA	34	A42D (GCC→GAC)	fixC	putative oxidoreductase FixC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/16);  total (18/16)

CTCTTATGTCCGAAGATATCTTTGACGCCATCATCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGCCAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGAACACATTATTCCTGGTTTCGCCGACTCCGCCCCCGTAGAACGCCTGATCACCCATGAAAAACTCGCGTTTATGACGGAAAAGTCAGCGATGACT  >  NC_000913/44175‑44440                                                                                                                                  |                                                                                                                                         ctctTATGTCCGAAGATATCTTTGACGCCATCATCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGAGACAAGAACGTc                                                                                                                                >  2:485807/1‑140 (MQ=255)   ctTATGTCCGAAGATATCTTTGACGCCATCATCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCAc                                                                                                                              >  1:80205/1‑140 (MQ=255)           cGAAGATATCTTTGACGCCATCATCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCggcgggc                                                                                                                      <  2:194249/140‑1 (MQ=255)              aGATATCTTTGACGCCATCATCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGACACCGGCGGGCGtc                                                                                                                   >  2:253171/1‑140 (MQ=255)                 tatCTTTGACGCCATCATCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTctct                                                                                                                <  1:485807/140‑1 (MQ=255)                   tCTTTGACGCCATCATCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTAt                                                                                                              >  1:663860/1‑140 (MQ=255)                       tGACGCCATCATCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGccc                                                                                                          >  1:596672/1‑140 (MQ=255)                        gACGCCATCATCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCca                                                                                                         <  1:449302/140‑1 (MQ=255)                        gACGCCATCATCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCca                                                                                                         <  2:397193/140‑1 (MQ=255)                            ccATCATCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGt                                                                                                     >  2:633406/1‑140 (MQ=255)                             catcatCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCca                                                                                                         <  2:377361/135‑1 (MQ=255)                             catcatCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCca                                                                                                         >  1:355576/1‑135 (MQ=255)                             catcatCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCca                                                                                                         >  1:377361/1‑135 (MQ=255)                             catcatCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCca                                                                                                         <  2:355576/135‑1 (MQ=255)                                  tcgtcgGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGaa                                                                                               <  1:357030/140‑1 (MQ=255)                                       ggTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGAACACat                                                                                          >  1:567787/1‑140 (MQ=255)                                                   gCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGTCCCCCGGCTGGAACACATTATTCCCGGttt                                                                              >  1:17262/1‑140 (MQ=255)                                                   gCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGAACACATTATTCCTGGttt                                                                              >  1:85401/1‑140 (MQ=255)                                                              tGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGAACACATTATTCCTGGTTTCGCCGACTCCg                                                                   <  1:633406/140‑1 (MQ=255)                                                                         tGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGAACACATTATTCCTGGTTTCGCCGACTCCGCCCCCGTAGaa                                                        <  2:80205/140‑1 (MQ=255)                                                                             cGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGAACACATTATTCCTGGTTTCGCCGACTCCg                                                                   <  2:173454/125‑1 (MQ=255)                                                                             cGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGAACACATTATTCCTGGTTTCGCCGACTCCg                                                                   >  1:173454/1‑125 (MQ=255)                                                                             cGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGAACACATTATTCCTGGTTTCGCCGACTCCGCCCCCGTAGAACGcc                                                    >  2:274915/1‑140 (MQ=255)                                                                                          gcgcAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGCCTCTATGCCCACCGTCTGGGACACAATATTCCTGGTTTCGCCCACTCCCCCCCCGCAGAACGCCTGATCACCCCTGa                                       >  2:584076/1‑140 (MQ=255)                                                                                                gtgtTAGTTATCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGAACACATTATTCCTGGTTTCGCCGACTCCGCCCCCGTAGAACGCCTGATCACCCATGAAAAACt                                 <  1:274915/140‑1 (MQ=255)                                                                                                          tCGAGCGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGGCTCTATGCCCACAGTCTGGAACACATTATTCCTGGTTTCGCCGACTCCGCCCCCGTAGAACGCCTGATCACCCATGAAAAACTCGCGTTTATg                       >  2:225471/1‑140 (MQ=255)                                                                                                               cGTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGAAAACATTATTCCTGGGTTCGCCGACCCCCCCCCCGTAGAACGCCTGATCACCCCTGAAAAAATCGCGGTTATGACGGa                  >  2:372487/1‑140 (MQ=255)                                                                                                                gTGGCAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGAACACATTATTCCTGGTTTCGCCGACTCCGCCCCCGTAGAACGCCTGATCACCCATGAAAAACTCGCGTTTATGACGGaa                 >  1:217877/1‑140 (MQ=255)                                                                                                                    cAATTCCGCAGGTGACAAGAACGTCACCGGCGGGCGGCTCTATGCCCACAGTCTGGAAAACATTATTCCTGGGTTCGCCGACTCCCCCCCCGTAGAACGCCTGATCACCCATGAAAAACTCGCGTTTATGACGGAAAAGt             >  2:688301/1‑140 (MQ=255)                                                                                                                        tCCGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCCCAGGCTGGGACACATTATTCCTGGTTTCGCCCACTCCGCCCCCGTAGAACGCCCGATCACCCATGAAAAACCCCCGTTTTTGACGGAAAAagcagc         >  2:209535/1‑140 (MQ=255)                                                                                                                          cGCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGAACACATTATTCCTGGTTTCGCCGACTCCGCCCCCGTAGAACGCCTGATCACCCATGAAAAACTCGCGTTTATGACGGAAAAGTCAGCGa       <  2:567787/140‑1 (MQ=255)                                                                                                                           gCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGAACACATTATTCCTGGTTTCGCCGACTCCGCCCCCGTAGAACGCCTGATCACCCATGAAAAACTCGCGTTTATGACGGAAAAGTCAGCGAt      <  2:596672/140‑1 (MQ=255)                                                                                                                           gCAGGTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGAACACATTATTCCTGGTTTCGCCGACTCCGCCCCCGTAGAACGCCTGATCACCCATGAAAAACTCGCGTTTATGACGGAAAAGTCAGCGAt      <  1:108744/140‑1 (MQ=255)                                                                                                                               gTGACAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGAACACATTATTCCTGGTTTCGCCGACTCCGCCCCCGTAGAACGCCTGATCACCCATGAAAAACTCGCGTTTATGACGGAAAAGTCAGCGATGACt  <  2:17262/140‑1 (MQ=255)                                                                                                                               gTGACAAGAACGACACCGGCGGGCGTCTCTATGCCCACAGTCTGGAACACATTATTCCTGGTTTCGCCGACTCCGCCCCCGTAGAACGCCTGATCACCCATGAAAAACTCGCGTTTATGACGGAAAAGTCAGCGATGACt  <  1:253171/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                         CTCTTATGTCCGAAGATATCTTTGACGCCATCATCGTCGGTGCAGGGCTTGCCGGTTCGGTTGCCGCACTGGTGCTCGCCCGCGAAGGTGCGCAAGTGTTAGTTATCGAGCGTGGCAATTCCGCAGGTGCCAAGAACGTCACCGGCGGGCGTCTCTATGCCCACAGTCTGGAACACATTATTCCTGGTTTCGCCGACTCCGCCCCCGTAGAACGCCTGATCACCCATGAAAAACTCGCGTTTATGACGGAAAAGTCAGCGATGACT  >  NC_000913/44175‑44440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑