Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	673,612	T→C	D391G (GAC→GGC)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	673,612	0	T	C	100.0%	112.2 / NA	33	D391G (GAC→GGC)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/20);  total (13/20)

ACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTAT  >  NC_000913/673480‑673731                                                                                                                                     |                                                                                                                        aCCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGAcc                                                                                                                  >  1:296570/1‑140 (MQ=255) aCCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGAcc                                                                                                                  >  1:463008/1‑140 (MQ=255)         aaTCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACt                                                                                                          <  2:550605/140‑1 (MQ=255)            cGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGc                                                                                                       <  2:15264/140‑1 (MQ=255)              gcgcgcCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCa                                                                                                     <  1:402490/140‑1 (MQ=255)               cgcgcCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCag                                                                                                    >  1:553772/1‑140 (MQ=255)                       aGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACCCCCATCGCCGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGGAc                                                                                            >  2:312727/1‑140 (MQ=255)                             gCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACg                                                                                      <  2:116527/140‑1 (MQ=255)                              cTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGc                                                                                     >  1:254597/1‑140 (MQ=255)                              cTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGc                                                                                     <  2:183699/140‑1 (MQ=255)                              cTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGc                                                                                     >  1:656072/1‑140 (MQ=255)                              cTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGc                                                                                     >  1:142670/1‑140 (MQ=255)                              cTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGc                                                                                     >  1:394502/1‑140 (MQ=255)                                   gggAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCtttt                                                                                <  2:553772/140‑1 (MQ=255)                                           ccccAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTtcagtcag                                                                        >  2:360467/1‑140 (MQ=255)                                              cAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGc                                                                     <  2:254597/140‑1 (MQ=255)                                               aGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCt                                                                    >  2:544552/1‑140 (MQ=255)                                                  cgcgCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGc                                                                 <  2:656906/140‑1 (MQ=255)                                                        ggCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGaa                                                           <  1:360467/140‑1 (MQ=255)                                                                      ttACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCaga                                             >  2:377111/1‑140 (MQ=255)                                                                          gCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGACTCAGAGCCg                                         >  2:448117/1‑140 (MQ=255)                                                                              gCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAg                                     <  1:337267/140‑1 (MQ=255)                                                                                        aTCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGAt                           <  2:296570/140‑1 (MQ=255)                                                                                               tcagtTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCa                                      <  1:638747/122‑1 (MQ=255)                                                                                               tcagtTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCa                                      >  2:638747/1‑122 (MQ=255)                                                                                               tcagtTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGt                    <  2:394502/140‑1 (MQ=255)                                                                                                 agtTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGttt                  <  2:463008/140‑1 (MQ=255)                                                                                                  gtTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTg                 <  1:309109/140‑1 (MQ=255)                                                                                                       tCGGCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTGATGtt            >  1:411597/1‑140 (MQ=255)                                                                                                         ggCGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTGATGTTCa          <  1:448117/140‑1 (MQ=255)                                                                                                           cGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTGATGTTCAgg        <  2:411597/140‑1 (MQ=255)                                                                                                           cGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTGATGTTCAgg        <  1:664796/140‑1 (MQ=255)                                                                                                           cGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTGATGTTCAgg        <  1:544552/140‑1 (MQ=255)                                                                                                           cGATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTGATGTTCAgg        >  2:582975/1‑140 (MQ=255)                                                                                                            gATGGCGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGc       <  2:656072/140‑1 (MQ=255)                                                                                                                 cGTTGAAGGCCGCTTCATGGCCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTAt  >  1:469605/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                        ACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGATGGCGTTGAAGGCCGCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTAT  >  NC_000913/673480‑673731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑