Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	701,939	A→G	V271V (GTT→GTC)	nagA ←	N‑acetylglucosamine‑6‑phosphate deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	701,939	0	A	G	100.0%	243.2 / NA	68	V271V (GTT→GTC)	nagA	N‑acetylglucosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/39);  total (29/39)

ACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGAC  >  NC_000913/701802‑702075                                                                                                                                          |                                                                                                                                         acgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTCTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGAc                                                                                                                                        <  2:503668/140‑1 (MQ=255)   gcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCa                                                                                                                                      >  2:218601/1‑140 (MQ=255)    ccTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAg                                                                                                                                     >  2:58672/1‑140 (MQ=255)    ccTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAg                                                                                                                                     >  2:577100/1‑140 (MQ=255)       tCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGaca                                                                                                                                  <  1:558291/140‑1 (MQ=255)               ggTTAAGGATGAACCGCTTATCGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGt                                                                                                                          >  1:243271/1‑140 (MQ=255)               ggTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGt                                                                                                                          <  1:520745/140‑1 (MQ=255)               ggTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGt                                                                                                                          <  2:545249/140‑1 (MQ=255)               ggTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGt                                                                                                                          >  1:400821/1‑140 (MQ=255)                   aaGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGcc                                                                                                                      >  2:358067/1‑140 (MQ=255)                     ggATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCtt                                                                                                                    >  2:151565/1‑140 (MQ=255)                     ggATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCtt                                                                                                                    >  1:663312/1‑140 (MQ=255)                         gAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAg                                                                                                                <  1:691489/140‑1 (MQ=255)                          aaCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGa                                                                                                               >  2:486325/1‑140 (MQ=255)                           aCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGAc                                                                                                              >  2:108445/1‑140 (MQ=255)                           aCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGAc                                                                                                              <  2:269163/140‑1 (MQ=255)                           aCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGAc                                                                                                              >  1:263647/1‑140 (MQ=255)                             cGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGt                                                                                                            <  2:296064/140‑1 (MQ=255)                               cTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGttt                                                                                                          >  2:583609/1‑140 (MQ=255)                                ttAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTa                                                                                                         >  1:589223/1‑140 (MQ=255)                                ttAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCACCTTTCAGACGTTTa                                                                                                         >  2:508570/1‑140 (MQ=255)                                 tAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAg                                                                                                        <  2:242741/140‑1 (MQ=255)                                       aCCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCGGACGTTTAGCGTTGc                                                                                                  >  1:507454/1‑140 (MQ=255)                                        ccGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCg                                                                                                 <  1:58672/140‑1 (MQ=255)                                            tctcATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATg                                                                                             >  2:358903/1‑140 (MQ=255)                                                  ccACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCg                                                                                       <  2:724087/140‑1 (MQ=255)                                                   cacacaAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGt                                                                                      <  1:358067/140‑1 (MQ=255)                                                     cacaAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTaa                                                                                    >  1:695306/1‑140 (MQ=255)                                                      acaAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAAt                                                                                   <  1:108445/140‑1 (MQ=255)                                                              cGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATc                                                                                  >  1:341414/1‑133 (MQ=255)                                                              cGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATc                                                                                  <  2:341414/133‑1 (MQ=255)                                                               gTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCa                                                                          <  2:120562/140‑1 (MQ=255)                                                                ttACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAg                                                                         >  2:256485/1‑140 (MQ=255)                                                                ttACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTCTAGCGTTGCGAATGTTGGCGTAATCAACATGCAg                                                                         >  1:150392/1‑140 (MQ=255)                                                                 tACGGTAGTATATTGTTTTACCCGCAAAAGTGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAgg                                                                        <  2:625275/140‑1 (MQ=255)                                                                 tACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTCTAGCGTTGCGAATGTTGGCGTAATCAACATGCAgg                                                                        <  2:150392/140‑1 (MQ=255)                                                                   cGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGcc                                                                      <  1:577100/140‑1 (MQ=255)                                                                   cGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGcc                                                                      <  1:41571/140‑1 (MQ=255)                                                                    ggTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCGGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCa                                                                     <  2:507454/140‑1 (MQ=255)                                                                    ggTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGcga                                                                     >  1:728544/1‑138 (MQ=255)                                                                    ggTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCa                                                                     <  1:22515/140‑1 (MQ=255)                                                                    ggTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCa                                                                     <  1:559557/140‑1 (MQ=255)                                                                      tagtaTATTGTTTTACCCGCACAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATc                                                                   <  2:663312/140‑1 (MQ=255)                                                                      tagtaTATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAAt                                                                                   <  2:241140/124‑1 (MQ=255)                                                                      tagtaTATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAAt                                                                                   >  1:241140/1‑124 (MQ=255)                                                                      tagtaTATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCGATc                                                                   <  2:728544/140‑1 (MQ=255)                                                                      tagtaTATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATc                                                                   <  2:400821/140‑1 (MQ=255)                                                                      tagtaTATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATc                                                                   <  2:659939/140‑1 (MQ=255)                                                                      tagtaTATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATc                                                                   <  2:589223/140‑1 (MQ=255)                                                                       agtaTATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCa                                                                  <  2:695306/140‑1 (MQ=255)                                                                              tGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAAACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCaataa                                                           >  1:636811/1‑140 (MQ=255)                                                                                ttttACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATc                                                                   <  2:262105/130‑1 (MQ=255)                                                                                ttttACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATc                                                                   >  1:262105/1‑130 (MQ=255)                                                                                ttttACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAataata                                                         >  1:387644/1‑140 (MQ=255)                                                                                  ttACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATAcc                                                       >  2:437437/1‑140 (MQ=255)                                                                                   tACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCg                                                      <  2:243271/140‑1 (MQ=255)                                                                                     cccGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATc                                                                   <  1:437786/125‑1 (MQ=255)                                                                                     cccGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATc                                                                   >  2:437786/1‑125 (MQ=255)                                                                                                  aCTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCt                                       <  1:284159/140‑1 (MQ=255)                                                                                                  aCTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCt                                       <  2:387644/140‑1 (MQ=255)                                                                                                  aCTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCt                                       <  1:151565/140‑1 (MQ=255)                                                                                                  aCTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCt                                       <  1:508570/140‑1 (MQ=255)                                                                                                                gCACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATcgc                         >  2:318681/1‑140 (MQ=255)                                                                                                                 cACCTGCTGGCGCGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATcgcg                        <  1:571668/140‑1 (MQ=255)                                                                                                                          gcgcGGTGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCgccagg               <  1:318681/140‑1 (MQ=255)                                                                                                                                tGGCGTCAGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGt         >  1:55669/1‑140 (MQ=255)                                                                                                                                       aGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGAc  <  2:263647/140‑1 (MQ=255)                                                                                                                                       aGTGACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGAc  <  2:367137/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         ACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGAC  >  NC_000913/701802‑702075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑