Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	839,802	A→G	V577A (GTT→GCT)	fiu ←	putative iron siderophore outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	839,802	0	A	G	100.0%	165.7 / NA	48	V577A (GTT→GCT)	fiu	putative iron siderophore outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/24);  total (24/24)

CCCGGCCACGGATATCTCATAGCCTTCGACGCGTTTCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAACCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGT  >  NC_000913/839666‑839937                                                                                                                                         |                                                                                                                                        cccGGCCACGGATATCTCATAGCCTTCGACGCGTTTCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCt                                                                                                                                      >  2:713054/1‑140 (MQ=255) cccGGCCACGGATATCTCATAGCCTTCGACGCGTTTCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCt                                                                                                                                      >  1:350479/1‑140 (MQ=255)       cACGGATATCTCATAGCCTTCGACGCGTTTCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTACCAtt                                                                                                                                <  1:713054/140‑1 (MQ=255)          ggATATCTCATAGCCTTCGACGCGTTTCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCGTTTgg                                                                                                                             <  2:636397/140‑1 (MQ=255)                     aGCCTTCGGCGCGGTTCTTACTGTATTGCGAGTAAGATCAGTCATCGTGCTGCTAAACTTCATTTTCCATATCAGTGCGGAAAAGCGCGGCAGTGAGCAGCAGACCCTTATCCGGAGACTGCCATTTGGTGCCAATCTCg                                                                                                                  <  2:430713/140‑1 (MQ=255)                      gCCTTCGACGCGTTTCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGc                                                                                                                 <  2:418326/140‑1 (MQ=255)                       ccTTCGACGCGTTTCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCt                                                                                                                >  2:416831/1‑140 (MQ=255)                         ttCGACGCGTTTCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTgg                                                                                                              >  1:688725/1‑140 (MQ=255)                         ttCGACGCGTTTCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTgg                                                                                                              >  1:43649/1‑140 (MQ=255)                           cGACGCGTTTCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGgtg                                                                                                            >  2:413698/1‑140 (MQ=255)                            gACGCGTTTCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGgtgt                                                                                                           >  2:83397/1‑140 (MQ=255)                                  tttCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCtt                                                                                                     >  2:231680/1‑140 (MQ=255)                                  cttCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCtt                                                                                                     >  1:129195/2‑140 (MQ=255)                                            tATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGTGGttt                                                                                           >  1:124273/1‑140 (MQ=255)                                             aTTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTg                                                                                                 >  2:113757/1‑133 (MQ=255)                                             aTTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTg                                                                                                 <  1:113757/133‑1 (MQ=255)                                             aTTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTa                                                                                          >  1:359436/1‑140 (MQ=255)                                             aTTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTa                                                                                          >  1:372145/1‑140 (MQ=255)                                                 cGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAAt                                                                                      >  2:423759/1‑140 (MQ=255)                                                             gTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGTGGTTTAAAATCGGTGCGGTTgg                                                                          <  2:124273/140‑1 (MQ=255)                                                                  catTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTg                                                                     >  1:368887/1‑140 (MQ=255)                                                                      ttGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTAc                                                                 <  1:416831/140‑1 (MQ=255)                                                                            aaCTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGc                                                           >  1:193253/1‑140 (MQ=255)                                                                                      tCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTgcgc                                                 <  2:52566/140‑1 (MQ=255)                                                                                        gATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCaa                                               >  1:42874/1‑140 (MQ=255)                                                                                         atatCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTg                                                            >  1:511551/1‑126 (MQ=255)                                                                                         atatCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTg                                                            <  2:511551/126‑1 (MQ=255)                                                                                               gTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGaa                                        >  2:529721/1‑140 (MQ=255)                                                                                                      aCAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGttgttg                                 <  2:359436/140‑1 (MQ=255)                                                                                                        aGCGCGGCGGTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTgcc                               >  2:491565/1‑140 (MQ=255)                                                                                                              gcggTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCgg                         <  1:529721/140‑1 (MQ=255)                                                                                                                 gTGAGCAACAGACGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAgg                      <  1:491565/140‑1 (MQ=255)                                                                                                                            aCGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGaaa           >  2:123852/1‑140 (MQ=255)                                                                                                                            aCGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGaaa           >  1:159433/1‑140 (MQ=255)                                                                                                                            aCGTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGaaa           <  2:42874/140‑1 (MQ=255)                                                                                                                              gTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACg         <  2:390420/140‑1 (MQ=255)                                                                                                                              gTTTATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACg         >  1:390420/1‑140 (MQ=255)                                                                                                                                ttATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGc       <  1:423759/140‑1 (MQ=255)                                                                                                                                ttATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGc       <  2:368887/140‑1 (MQ=255)                                                                                                                                ttATCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGc       <  2:193253/140‑1 (MQ=255)                                                                                                                                 tATCCAGAGCCTGCCATTCGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCa      <  2:350479/140‑1 (MQ=255)                                                                                                                                  aTCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCAt     <  2:129195/140‑1 (MQ=255)                                                                                                                                   tCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGgctgc                  <  2:107044/126‑1 (MQ=255)                                                                                                                                   tCCAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGgctgc                  >  1:107044/1‑126 (MQ=255)                                                                                                                                    ccAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAg   <  1:231680/140‑1 (MQ=255)                                                                                                                                    ccAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAg   <  2:159433/140‑1 (MQ=255)                                                                                                                                     cAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGt  <  1:83397/140‑1 (MQ=255)                                                                                                                                     cAGAGCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGt  <  1:413698/140‑1 (MQ=255)                                                                                                                                      agagCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCAt     <  2:379795/136‑1 (MQ=255)                                                                                                                                      agagCCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCAt     >  1:379795/1‑136 (MQ=255)                                                                                                                                         |                                                                                                                                        CCCGGCCACGGATATCTCATAGCCTTCGACGCGTTTCTTACCGTATTGCGAGTAAGTTCCGTCATCATTTTGCTCAACTTCATTTTCGATATCAGTGCGGAACAGCGCGGCGGTGAGCAACAGACGTTTATCCAGAACCTGCCATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGT  >  NC_000913/839666‑839937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑