Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,002,129	A→G	Y108C (TAC→TGC)	elfG →	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,002,129	0	A	G	100.0%	141.1 / NA	41	Y108C (TAC→TGC)	elfG	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/20);  total (21/20)

GTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGT  >  NC_000913/1001993‑1002256                                                                                                                                         |                                                                                                                                gTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCaa                                                                                                                              >  1:357342/1‑140 (MQ=255)      aGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTa                                                                                                                         >  1:529867/1‑140 (MQ=255)                      tGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTgccgc                                                                                                         >  1:391939/1‑140 (MQ=255)                                               tttCCTGTGATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGc                                                                                <  2:529867/140‑1 (MQ=255)                                               tttCCTGTGATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGc                                                                                <  2:391939/140‑1 (MQ=255)                                                ttCCTGTGATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCa                                                                               >  1:676955/1‑140 (MQ=255)                                                  ccTGTGATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGt                                                                             >  2:261587/1‑140 (MQ=255)                                                    tgtgATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGc                                                                                           >  1:266843/1‑124 (MQ=255)                                                    tgtgATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGc                                                                                           <  2:266843/124‑1 (MQ=255)                                                    tgtgATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTtc                                                                           >  1:17399/1‑140 (MQ=255)                                                    tgtgATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTtc                                                                           >  2:191633/1‑140 (MQ=255)                                                    tgtgATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTtc                                                                           >  1:543422/1‑140 (MQ=255)                                                    tgtgATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTtc                                                                           >  1:564097/1‑140 (MQ=255)                                                       gATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTcc                                                                        >  2:216787/1‑140 (MQ=255)                                                       gATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTcc                                                                        <  2:564097/140‑1 (MQ=255)                                                           gCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACt                                                                    <  2:17399/140‑1 (MQ=255)                                                            cGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAg                                                                              >  1:520999/1‑129 (MQ=255)                                                            cGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAg                                                                              <  2:520999/129‑1 (MQ=255)                                                            cGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTg                                                                   >  2:357748/1‑140 (MQ=255)                                                                             gAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGATGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAAc                                                  >  2:727818/1‑140 (MQ=255)                                                                               aGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGt                                                <  2:226166/140‑1 (MQ=255)                                                                                gTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTg                                               <  1:261587/140‑1 (MQ=255)                                                                                   gCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGc                                            >  2:135812/1‑140 (MQ=255)                                                                                   gCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGc                                            >  1:343931/1‑140 (MQ=255)                                                                                   gCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGc                                            >  1:34626/1‑140 (MQ=255)                                                                                    ccGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGca                                           >  1:579290/1‑140 (MQ=255)                                                                                       aTGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAg                                        <  1:338509/140‑1 (MQ=255)                                                                                        tGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAgg                                       <  2:711095/140‑1 (MQ=255)                                                                                        tGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAgg                                       <  2:343931/140‑1 (MQ=255)                                                                                                     cgGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGa                          <  1:216787/140‑1 (MQ=255)                                                                                                     cgGATTCACCGTTAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGa                          <  2:543422/140‑1 (MQ=255)                                                                                                                 tAGTCTATTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCAGATGGCATATTTGc              <  2:34626/140‑1 (MQ=255)                                                                                                                       aTTTAGGCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCt        <  2:357342/140‑1 (MQ=255)                                                                                                                             gCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt  <  1:357748/140‑1 (MQ=255)                                                                                                                             gCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt  <  2:676955/140‑1 (MQ=255)                                                                                                                             gCGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt  >  1:627074/1‑140 (MQ=255)                                                                                                                             gCGACAACTGATGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt  <  1:727818/140‑1 (MQ=255)                                                                                                                              cGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt  >  2:251654/1‑139 (MQ=255)                                                                                                                              cGACAACTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt  <  1:251654/139‑1 (MQ=255)                                                                                                                                  aaCTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt  >  1:575780/1‑135 (MQ=255)                                                                                                                                  aaCTGGTGCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt  <  2:575780/135‑1 (MQ=255)                                                                                                                                         |                                                                                                                                GTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGT  >  NC_000913/1001993‑1002256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑