Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	49,370	T→C	G534G (GGT→GGC)	kefC →	K(+) : H(+) antiporter KefC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	49,370	0	T	C	100.0%	129.1 / NA	37	G534G (GGT→GGC)	kefC	K(+) : H(+) antiporter KefC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/23);  total (14/23)

CGCATTTGCAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGTCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATG  >  NC_000913/49234‑49502                                                                                                                                         |                                                                                                                                     cGCATTTGCAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTg                                                                                                                                   <  1:132076/140‑1 (MQ=255) cGCATTTGCAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTg                                                                                                                                   <  1:352328/140‑1 (MQ=255)    aTTTGCAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTgggg                                                                                                                                <  2:122755/140‑1 (MQ=255)              tattGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAg                                                                                                                      >  2:404744/1‑140 (MQ=255)                 tGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAgcgc                                                                                                                   <  1:258111/140‑1 (MQ=255)                 tGCCCGCGCC‑‑CGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCga                                                                                                                 >  1:640841/1‑140 (MQ=255)                        gcccgcgATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACg                                                                                                            >  1:500467/1‑140 (MQ=255)                             cgATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCg                                                                                                       <  1:62772/140‑1 (MQ=255)                                       cACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTccg                                                                                             <  1:446686/140‑1 (MQ=255)                                          tACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAGAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTccgccg                                                                                          >  2:635489/1‑140 (MQ=255)                                            cATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCt                                                                                        <  2:653867/140‑1 (MQ=255)                                              ttCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCttt                                                                                      <  1:647931/140‑1 (MQ=255)                                                   ttGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAatat                                                                                 >  2:505437/1‑140 (MQ=255)                                                    tGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATAtt                                                                                >  2:276925/1‑140 (MQ=255)                                                    tGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATAtt                                                                                <  2:500467/140‑1 (MQ=255)                                                      cGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCa                                                                              >  1:555333/1‑140 (MQ=255)                                                      cGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGCGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCa                                                                              >  1:187146/1‑140 (MQ=255)                                                        tCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGa                                                                            <  1:238743/140‑1 (MQ=255)                                                        tCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGa                                                                            <  1:238520/140‑1 (MQ=255)                                                           ggcaggcGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGAtgg                                                                         <  1:545622/140‑1 (MQ=255)                                                                           ccGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAAt                                                         >  2:178816/1‑140 (MQ=255)                                                                                     aaaCCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAAc                                               >  1:679567/1‑140 (MQ=255)                                                                                      aaCCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACg                                              <  1:721593/140‑1 (MQ=255)                                                                                                   gcgcTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTAGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCcg                                 >  1:494382/1‑140 (MQ=255)                                                                                                    cgcTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCcgc                                >  2:193445/1‑140 (MQ=255)                                                                                                        gAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCTCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCgcgg                            <  2:640841/140‑1 (MQ=255)                                                                                                        gAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCgcgg                            <  2:555333/140‑1 (MQ=255)                                                                                                        gAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCgcgg                            <  2:43845/140‑1 (MQ=255)                                                                                                        gAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCgcgg                            <  2:679567/140‑1 (MQ=255)                                                                                                        gAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCgcgg                            <  1:134678/140‑1 (MQ=255)                                                                                                                        gCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCGCGGCGGTCTATAAACGCAc            <  1:635489/140‑1 (MQ=255)                                                                                                                        gCACTGGAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCGCGGCGGTCTATAAACGCAc            <  1:404744/140‑1 (MQ=255)                                                                                                                              gAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCGCGGCGGTCTATAAACGCACCAgcgc      >  1:665838/1‑140 (MQ=255)                                                                                                                              gAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCGCGGCGGTCTATAAACGCACCAgcgc      >  1:451668/1‑140 (MQ=255)                                                                                                                              gAAAGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCGCGGCGGACTATAAACGCACCAgcgc      <  1:193445/140‑1 (MQ=255)                                                                                                                                aaGTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGa    <  1:276925/140‑1 (MQ=255)                                                                                                                                aaGTTTAGGCCTGGGGCAGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGa    <  2:187146/140‑1 (MQ=255)                                                                                                                                  gTTTAGGCCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTAGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATg  <  2:494382/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CGCATTTGCAGATTATTGCCCGCGCCCGCGATGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGTCTGGGGCCGTATGAAGCGCGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATG  >  NC_000913/49234‑49502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑