Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,019,564	A→G	W164R (TGG→CGG)	ompA ←	outer membrane porin A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,019,564	0	A	G	100.0%	126.4 / NA	37	W164R (TGG→CGG)	ompA	outer membrane porin A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/23);  total (14/23)

TCCGGTGCCGGAGCTGGAGCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCACTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGC  >  NC_000913/1019428‑1019695                                                                                                                                         |                                                                                                                                    tCCGGTGCCGGAGCTGGAGCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTg                                                                                                                                  <  1:179990/140‑1 (MQ=255)  ccGGTGCCGGAGCTGGAGCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTgg                                                                                                                                 >  1:130091/1‑140 (MQ=255)   cGGTGCCGGAGCTGGAGCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGt                                                                                                                                >  2:312472/1‑140 (MQ=255)     gTGCCGGAGCTGGAGCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTAt                                                                                                                              <  1:341939/140‑1 (MQ=255)                 gAGCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTa                                                                                                                  <  2:601995/140‑1 (MQ=255)                   gCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGc                                                                                                                <  2:701686/140‑1 (MQ=255)                   gCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGc                                                                                                                <  2:313490/140‑1 (MQ=255)                   gCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGc                                                                                                                <  2:229380/140‑1 (MQ=255)                        aGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGAttt                                                                                                           <  1:312472/140‑1 (MQ=255)                             ctactGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGa                                                                                                      >  1:161301/1‑140 (MQ=255)                              tactGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAg                                                                                                     <  2:655537/140‑1 (MQ=255)                              tactGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAg                                                                                                     <  1:302871/140‑1 (MQ=255)                                ctGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTg                                                                                                   <  2:266688/140‑1 (MQ=255)                                         ctTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTAc                                                                                          <  2:292162/140‑1 (MQ=255)                                                  gACCGAAACGGTAGGAAACCCCCAGGCTCAGCAGGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCg                                                                                 <  2:111315/140‑1 (MQ=255)                                                   aCCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGc                                                                                >  1:702455/1‑140 (MQ=255)                                                       aaaCGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGc                                                                            <  2:130091/140‑1 (MQ=255)                                                        aaCGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTa                                                                                           >  1:510781/1‑124 (MQ=255)                                                        aaCGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTa                                                                                           <  2:510781/124‑1 (MQ=255)                                                        aaCGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCg                                                                           >  2:104708/1‑140 (MQ=255)                                                                        cAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGc                                                           <  1:625718/140‑1 (MQ=255)                                                                         aGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGcc                                                          >  2:193311/1‑140 (MQ=255)                                                                          ggctgcgCATGCCGTTGTCCGGACGAGTGCCGAGGGTGTGTGCGTCCCCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTGGCGATTTCAGGCGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCg                                                         <  1:209065/134‑1 (MQ=255)                                                                          ggCTCAGCATGCCGTTGGCCGGGCGGGGGCCGGTGGGTGGGGGGGCACCGATGTTGTTTGTCCGCTTGTTTTCCAGGCGGGGAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCg                                                         <  2:442171/140‑1 (MQ=255)                                                                                gCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCg                                                   <  1:104708/140‑1 (MQ=255)                                                                                 cATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGt                                                  >  2:229476/1‑140 (MQ=255)                                                                                  aTGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTg                                                 <  2:161301/140‑1 (MQ=255)                                                                                    gCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGt                                               <  1:422709/140‑1 (MQ=255)                                                                                       gTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGtttt                                            <  1:229476/140‑1 (MQ=255)                                                                                         tGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTa                                          >  2:24379/1‑140 (MQ=255)                                                                                                               gtgtgCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAgtgt                    >  2:263851/1‑140 (MQ=255)                                                                                                               gtgtgCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAgtgt                    >  2:415330/1‑140 (MQ=255)                                                                                                                  tgCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTg                 >  2:389697/1‑140 (MQ=255)                                                                                                                  tgCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTg                 <  1:661511/140‑1 (MQ=255)                                                                                                                  tgCGTCACCGATGTTGTTGGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTg                 <  1:370226/140‑1 (MQ=255)                                                                                                                                 gttgGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATg   <  2:231640/139‑1 (MQ=255)                                                                                                                                 gttgGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATg   >  1:231640/1‑139 (MQ=255)                                                                                                                                 gttgGTCCGCTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGc  >  2:8884/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    TCCGGTGCCGGAGCTGGAGCCGGAGCAACTACTGGAGCTGCTTCGCCCTGACCGAAACGGTAGGAAACACCCAGGCTCAGCATGCCGTTGTCCGGACGAGTGCCGATGGTGTGTGCGTCACCGATGTTGTTGGTCCACTGGTATTCCAGACGGGTAGCGATTTCAGGAGTGATCGCGTACTCAACACCGCCAGCGAAGACCGGAGAAACGCCGGTGTCGTGGTTTTTACCATAAACGTTGGATTTAGTGTCTGCACGCCATACCATGC  >  NC_000913/1019428‑1019695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑