Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,016	T→C	R210R (CGT→CGC)	araC →	DNA‑binding transcriptional dual regulator AraC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,016	0	T	C	100.0%	167.1 / NA	49	R210R (CGT→CGC)	araC	DNA‑binding transcriptional dual regulator AraC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/26);  total (23/26)

AGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTT  >  NC_000913/70881‑71151                                                                                                                                        |                                                                                                                                        aGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGt                                                                                                                                     <  1:719247/140‑1 (MQ=255)          cGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCttt                                                                                                                            >  1:592197/1‑140 (MQ=255)                gCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGcc                                                                                                                      >  2:2872/1‑140 (MQ=255)                 cTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCca                                                                                                                     <  2:520561/140‑1 (MQ=255)                      tccaCCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGt                                                                                                                <  2:14430/140‑1 (MQ=255)                           cGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAggg                                                                                                           >  2:598316/1‑140 (MQ=255)                            gATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCATACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGa                                                                                                          >  1:564306/1‑140 (MQ=255)                            gATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGa                                                                                                          <  1:612620/140‑1 (MQ=255)                              tGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGAtt                                                                                                        >  2:362715/1‑140 (MQ=255)                                      cGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCtt                                                                                                <  2:25493/140‑1 (MQ=255)                                         gTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAg                                                                                             <  2:307656/140‑1 (MQ=255)                                          tACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGc                                                                                            >  2:382643/1‑140 (MQ=255)                                          tACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGc                                                                                            >  2:658126/1‑140 (MQ=255)                                          tACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGc                                                                                            >  2:101480/1‑140 (MQ=255)                                                 ggCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGcgcg                                                                                     <  2:542987/140‑1 (MQ=255)                                                    ttGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAgg                                                                                  >  1:184496/1‑140 (MQ=255)                                                      gTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGAc                                                                                <  1:556506/140‑1 (MQ=255)                                                      gTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGAc                                                                                >  2:556506/1‑140 (MQ=255)                                                      gTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGAc                                                                                <  1:175711/140‑1 (MQ=255)                                                        cAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCa                                                                              <  1:362715/140‑1 (MQ=255)                                                        cAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCa                                                                              <  2:575850/140‑1 (MQ=255)                                                        cAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCAAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCa                                                                              <  1:68019/140‑1 (MQ=255)                                                                 aGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAg                                                                     >  2:74874/1‑140 (MQ=255)                                                                  gCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGt                                                                    <  1:2872/140‑1 (MQ=255)                                                                   cGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTc                                                                   >  2:606933/1‑140 (MQ=255)                                                                     aTCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAg                                                                 >  2:451639/1‑140 (MQ=255)                                                                     aTCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAg                                                                 >  1:417822/1‑140 (MQ=255)                                                                         ccTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGa                                                             <  1:382643/140‑1 (MQ=255)                                                                           tGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAg                                                           <  1:74874/140‑1 (MQ=255)                                                                           tGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAg                                                           <  2:184496/140‑1 (MQ=255)                                                                            ggCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgc                                                          >  1:667907/1‑140 (MQ=255)                                                                            ggCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgc                                                          <  2:728826/140‑1 (MQ=255)                                                                             gCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgct                                                         >  2:468452/1‑140 (MQ=255)                                                                                   aGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt                                                   <  1:598316/140‑1 (MQ=255)                                                                                      aaTTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAg                                                <  1:451639/140‑1 (MQ=255)                                                                                      aaTTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAg                                                <  2:564306/140‑1 (MQ=255)                                                                                           tGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTa                                           <  1:658126/140‑1 (MQ=255)                                                                                                      gCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCt                                <  2:592197/140‑1 (MQ=255)                                                                                                            cacaGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGa                                     <  2:286376/129‑1 (MQ=255)                                                                                                            cacaGCATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGa                                     >  1:286376/1‑129 (MQ=255)                                                                                                                 cATGTTTGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgt                     >  2:632659/1‑140 (MQ=255)                                                                                                                     tttGCTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcggt                 >  1:124115/1‑140 (MQ=255)                                                                                                                         cTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCa             >  1:76066/1‑140 (MQ=255)                                                                                                                         cTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCa             >  2:659750/1‑140 (MQ=255)                                                                                                                         cTTGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCCGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCa             >  2:254543/1‑140 (MQ=255)                                                                                                                          ttGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCaa            <  2:76066/140‑1 (MQ=255)                                                                                                                           tGTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAAt           >  2:688021/1‑140 (MQ=255)                                                                                                                            gTCGCCGTCGCGCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATg          <  1:688021/140‑1 (MQ=255)                                                                                                                                    cgcgCCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtttt  <  1:101480/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        AGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTT  >  NC_000913/70881‑71151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑