Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,313,218	A→G	V149A (GTT→GCT)	yciC ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,313,218	0	A	G	100.0%	135.6 / NA	39	V149A (GTT→GCT)	yciC	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/25);  total (14/25)

TACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAACGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGACTCTCTGACCCGCAGACACCAGCTGG  >  NC_000913/1313082‑1313352                                                                                                                                         |                                                                                                                                       tACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGa                                                                                                                                     >  1:108434/1‑140 (MQ=255) tACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGa                                                                                                                                     >  1:195685/1‑140 (MQ=255)   cTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGacc                                                                                                                                   <  2:66940/140‑1 (MQ=255)      cGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGaccacc                                                                                                                                >  1:546467/1‑140 (MQ=255)      cGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGACCAGTAAAATGGCCATGATAATTCCCGGAGCGaccacc                                                                                                                                >  2:153276/1‑140 (MQ=255)          tGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCa                                                                                                                            <  2:256216/140‑1 (MQ=255)          tGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCa                                                                                                                            <  1:436977/140‑1 (MQ=255)          tGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCa                                                                                                                            <  1:410080/140‑1 (MQ=255)          tGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGACCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCa                                                                                                                            <  1:153276/140‑1 (MQ=255)           gCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCGCATTTTGTCCTGTACCACCATCACCGGAGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCat                                                                                                                           >  2:651508/1‑140 (MQ=255)           gCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGac                                                                                                                                    <  1:476748/131‑1 (MQ=255)           gCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGac                                                                                                                                    >  2:476748/1‑131 (MQ=255)                    cGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAAt                                                                                                                  <  2:87542/140‑1 (MQ=255)                     gCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATc                                                                                                                 <  2:546467/140‑1 (MQ=255)                       atatTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTg                                                                                                               <  2:355990/140‑1 (MQ=255)                          ttCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTAc                                                                                                            >  2:218439/1‑140 (MQ=255)                             gCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTaa                                                                                                         >  1:302582/1‑140 (MQ=255)                                     cAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAg                                                                                                 <  2:142179/140‑1 (MQ=255)                                        ccGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTa                                                                                                          >  1:695556/1‑128 (MQ=255)                                        ccGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTa                                                                                                          <  2:695556/128‑1 (MQ=255)                                               cTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAAt                                                                                       <  2:396920/140‑1 (MQ=255)                                                tGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATc                                                                                      >  2:420097/1‑140 (MQ=255)                                                 gCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCa                                                                                     <  2:302582/140‑1 (MQ=255)                                                         tCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGaataaat                                                                             <  1:218439/140‑1 (MQ=255)                                                          cGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGaataaata                                                                            >  1:709951/1‑140 (MQ=255)                                                                aaaaaCGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTg                                                                      <  2:195685/140‑1 (MQ=255)                                                                              tGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCa                                                        <  2:108434/140‑1 (MQ=255)                                                                                     tACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCAc                                                 <  2:492313/140‑1 (MQ=255)                                                                                                      ccAGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCa                                >  1:268546/1‑140 (MQ=255)                                                                                                        aGGGCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACt                              <  1:195424/140‑1 (MQ=255)                                                                                                         gggCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTg                             <  2:365113/140‑1 (MQ=255)                                                                                                         gggCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTg                             <  2:268546/140‑1 (MQ=255)                                                                                                          ggCCAGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGa                            <  2:709951/140‑1 (MQ=255)                                                                                                              aGTAAAATGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGActct                        >  1:104398/1‑140 (MQ=255)                                                                                                                     tGGCCATGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGACTCTCTGAccc                 >  2:178916/1‑140 (MQ=255)                                                                                                                           tGATAATTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGACTCTCTGACCCGCAGac           <  1:178916/140‑1 (MQ=255)                                                                                                                                aTTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGACTCTCTGACCCGCAGACACCAg      <  2:4833/140‑1 (MQ=255)                                                                                                                                aTTCCCGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGACTCTCTGACCCGCAGACACCAg      <  2:52354/140‑1 (MQ=255)                                                                                                                                  tCCCGGAACGACCACCAGCACGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGCATAAATACCTTTGGCAATATCGGAGCACTGGCACCAATAGCTCGCAGCGCACTGCCTCTCTGACCCGCAGacagtagtt    <  2:97300/140‑7 (MQ=255)                                                                                                                                    ccGGAGCGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGACTCTCTGACCCGCAGACACCAGCTgg  <  1:651508/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       TACTGCGGGTGCCACCAGACGCATATTCGCCCAAGTCAGCCGCATACTGCTACGCATCGAGGCAAAAACGCCCATTTTGTCCTGTACCAACATCACCGGTGCCAGGGCCAGTAAAATGGCCATGATAATTCCCGGAACGACCACCAGCATGATGCCAATCTGTACTAAAAGGGTAGTCAGAAAAATCAGAATAAATAACTTTGGCAATATCGGCGCACTGGCACCAATAGCTCGCAGCGCACTGACTCTCTGACCCGCAGACACCAGCTGG  >  NC_000913/1313082‑1313352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑