Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,320,624	C→T	G254E (GGG→GAG)	trpD ←	anthranilate synthase subunit TrpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,320,624	0	C	T	100.0%	75.3 / NA	23	G254E (GGG→GAG)	trpD	anthranilate synthase subunit TrpD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/11);  total (12/11)

CGACAAACGCACTGGCGGTAGAAATATTGATACTGTTGCTGCCGTCACCGCCAGTACCGACGATATCAGCAAACAGATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCCCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTCCGGCTTCAGCTCGCCACGCACCACCGCTGAAAACAGCTGGTGGCTTTCTTGTTGGCTAAGCGTC  >  NC_000913/1320496‑1320752                                                                                                                                 |                                                                                                                                 cgACAAACGCACGGGGGGAAGAAATTTTGATCCTGTTCCTGCCGTCACCGCCAGTACCGACGATATCAGCAAACAGATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCg                                                                                                                       <  1:288046/140‑1 (MQ=255)    cAAACGCACTGGCGGTAGAAATATTGATACTGTTGCTGCCGTCACCGCCAGTACCGACGATATCAGCAAACAGATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTc                                                                                                                    >  2:440099/1‑140 (MQ=255)                                aCTGTTGCTGCCGTCACCGCCAGTACCGACGATATCAGCAAACAGATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCa                                                                                        >  2:41344/1‑140 (MQ=255)                                 cTGTTGCTGCCGTCACCGCCAGTACCGACGATATCAGCAAACAGATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCAc                                                                                       >  2:720086/1‑140 (MQ=255)                                   gTTGCTGCCGTCACCGCCAGTACCGACGATATCAGCAAACAGATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCa                                                                                     <  1:395258/140‑1 (MQ=255)                                   gTTGCTGCCGTCACCGCCAGTACCGACGATATCAGCAAACAGATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCa                                                                                     >  1:312870/1‑140 (MQ=255)                                                       tACCGACGATATCAGCAAACAGATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTcc                                                                 <  2:606565/140‑1 (MQ=255)                                                           gacgaTATCAGCAAACAGATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCa                                                                         >  1:717614/1‑128 (MQ=255)                                                           gacgaTATCAGCAAACAGATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCa                                                                         <  2:717614/128‑1 (MQ=255)                                                                   cAGCAAACAGATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTCCGGCTTCAGCTCg                                                     <  1:124872/140‑1 (MQ=255)                                                                        aaCAGATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTCCGGCTTCAGCTCGCCACg                                                >  2:729388/1‑140 (MQ=255)                                                                        aaCAGATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTCCGGCTTCAGCTCGCCACg                                                >  1:596169/1‑140 (MQ=255)                                                                           aGATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTCCGGCTTCAGCTCGCCACGcac                                             <  2:312870/140‑1 (MQ=255)                                                                            gATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTCCGGCTTCAGCTCGCCACGcacc                                            >  2:527886/1‑140 (MQ=255)                                                                            gATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTCCGGCTTCAGCTCGCCACGcacc                                            >  2:255091/1‑140 (MQ=255)                                                                               aaTCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTCCGGCTTCAGCTCGCCACGCaccacc                                         <  1:440099/140‑1 (MQ=255)                                                                                aTCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTCCGGCTTCAGCTCGCCACGCACCACCg                                        >  2:99843/1‑140 (MQ=255)                                                                                     ggCGCGGGGACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTCCGGCTTCAGCTCGCCACGCACCACCGCTGaa                                   >  2:11704/1‑140 (MQ=255)                                                                                              aCGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTCCGGCTTCAGCTCGCCACGCACCACCGCTGAAAACAGCtgg                          <  1:11704/140‑1 (MQ=255)                                                                                                   gcTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTCCGGCTTCAGCTCGCCACGCACCACCGCTGAAAACAGCTGGTGGCt                     <  1:704523/140‑1 (MQ=255)                                                                                                   gcTGCGTTTTCCAGTAGCGCGGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTCCGGCTTCAGCTCGCCACGCACCACCGCTGAAAACAGCTGGTGGCt                     <  1:41344/140‑1 (MQ=255)                                                                                                                     gcgGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTCCGGCTTCAGCTCGCCACGCACCACCGCTGAAAACAGCTGGTGGCTTTCTTGTTGGCTAAGCGt   <  1:729388/140‑1 (MQ=255)                                                                                                                      cgGTTGCTGCCTCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTCCGGCTTCAGCTCGCCACGCACCACCGCTGAAAACAGCTGGTGGCTTTCTTGTTGGCTAAGCGTc  >  2:13358/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                 CGACAAACGCACTGGCGGTAGAAATATTGATACTGTTGCTGCCGTCACCGCCAGTACCGACGATATCAGCAAACAGATAATCCGGGCGCGGGAACGGCGCTGCGTTTTCCAGTAGCGCGGTTGCTGCCCCGGCGATCTCGTTCGGGTGCTCACCGCGAATTTTCATGCTCACCAGCGCCGCCGCCAGTTGTTCCGGCTTCAGCTCGCCACGCACCACCGCTGAAAACAGCTGGTGGCTTTCTTGTTGGCTAAGCGTC  >  NC_000913/1320496‑1320752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑