Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,426,475	C→T	L8F (CTC→TTC)	ynaA →	Rac prophage; putative prophage tail length tape measure domain‑containing protein YnaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,426,475	0	C	T	100.0%	173.7 / NA	49	L8F (CTC→TTC)	ynaA	Rac prophage; putative prophage tail length tape measure domain‑containing protein YnaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/24);  total (25/24)

ATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAATAGCGCAGGGAAAGTGACTAAGGCAAC  >  NC_000913/1426340‑1426599                                                                                                                                        |                                                                                                                             aTCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGc                                                                                                                          <  1:78500/140‑1 (MQ=255) aTCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGc                                                                                                                          <  1:725018/140‑1 (MQ=255)  tCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCa                                                                                                                         >  2:586257/1‑140 (MQ=255)      tgatgCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCaataa                                                                                                                     >  2:367479/1‑140 (MQ=255)              cTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGAt                                                                                                             <  2:376277/140‑1 (MQ=255)                ggAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAatt                                                                                                                   >  1:65325/1‑132 (MQ=255)                ggAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAatt                                                                                                                   <  2:65325/132‑1 (MQ=255)                                tGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAaat                                                                                           <  2:157541/140‑1 (MQ=255)                                 ggCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAaata                                                                                          <  1:698306/140‑1 (MQ=255)                                       gTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTg                                                                                    <  1:367479/140‑1 (MQ=255)                                        tAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGa                                                                                   >  1:586955/1‑140 (MQ=255)                                               tataTCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGAc                                                                                  >  1:475126/1‑134 (MQ=255)                                               tataTCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGAc                                                                                  <  2:475126/134‑1 (MQ=255)                                                      actaTTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGAcctcct                                                                     >  2:597876/1‑140 (MQ=255)                                                           tatGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTa                                                                >  1:51657/1‑140 (MQ=255)                                                             tGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGt                                                              >  1:553885/1‑140 (MQ=255)                                                             tGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGt                                                              >  2:300178/1‑140 (MQ=255)                                                             tGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGt                                                              >  1:246093/1‑140 (MQ=255)                                                             tGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGt                                                              >  2:551572/1‑140 (MQ=255)                                                                 gCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCaaa                                                          >  1:455342/1‑140 (MQ=255)                                                                  ccTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGAc                                                                          <  2:703075/123‑1 (MQ=255)                                                                  ccTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGAc                                                                          >  1:703075/1‑123 (MQ=255)                                                                  ccTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCaaaa                                                         >  2:450206/1‑140 (MQ=255)                                                                  ccTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCaaaa                                                         >  2:557185/1‑140 (MQ=255)                                                                  ccTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCaaaa                                                         >  2:96723/1‑140 (MQ=255)                                                                  ccTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCaaaa                                                         >  1:676586/1‑140 (MQ=255)                                                                  ccTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCaaaa                                                         >  1:43092/1‑140 (MQ=255)                                                                  ccTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCaaaa                                                         >  1:405011/1‑140 (MQ=255)                                                                      aaaCCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGAc                                                     <  1:118545/140‑1 (MQ=255)                                                                       aaCCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACg                                                    <  2:231001/140‑1 (MQ=255)                                                                       aaCCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACg                                                    <  1:557185/140‑1 (MQ=255)                                                                                    gTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGaaa                                       >  2:651787/1‑140 (MQ=255)                                                                                    gTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGaaa                                       <  2:43092/140‑1 (MQ=255)                                                                                              tgttgtCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCaaa                             <  1:551572/140‑1 (MQ=255)                                                                                                 tgtCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAATAg                          >  1:552970/1‑140 (MQ=255)                                                                                                  gtCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAATAgc                         >  2:186512/1‑140 (MQ=255)                                                                                                   tCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAGTAgcg                        <  2:553885/140‑1 (MQ=255)                                                                                                   tCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAATAgcg                        >  2:148486/1‑140 (MQ=255)                                                                                                     aGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAAt                            <  2:220843/134‑1 (MQ=255)                                                                                                     aGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAAt                            >  1:220843/1‑134 (MQ=255)                                                                                                     aGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAATAGCGCa                      <  1:96723/140‑1 (MQ=255)                                                                                                           tttAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAATAGCGCAGGGaaa                <  1:651787/140‑1 (MQ=255)                                                                                                           tttAATAAATGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAATAGCGCAGGGaaa                <  2:676586/140‑1 (MQ=255)                                                                                                                 aaaTGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAATAGCGCAGGGAAAGTGACt          <  1:526579/140‑1 (MQ=255)                                                                                                                   aTGGCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAATAGCGCAGGGAAAGTGACTaa        <  2:16845/140‑1 (MQ=255)                                                                                                                      gCAGAGCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAATAGCGCAGGGAAAGTGACTAAGGc     <  2:51657/140‑1 (MQ=255)                                                                                                                         gagCAAACCTCGCGTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAATAGCGCAGGGAAAGTGACTAAGGCAAc  <  1:300178/140‑1 (MQ=255)                                                                                                                                    gcgTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAATAGCGCAGGGAAAGTGACTAAGGCaa   >  2:189547/1‑128 (MQ=255)                                                                                                                                    gcgTTTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAATAGCGCAGGGAAAGTGACTAAGGCaa   <  1:189547/128‑1 (MQ=255)                                                                                                                                        |                                                                                                                             ATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGCTGCAAATAGCGCAGGGAAAGTGACTAAGGCAAC  >  NC_000913/1426340‑1426599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑