Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	92,082	A→G	T224A (ACT→GCT)	ftsI →	peptidoglycan DD‑transpeptidase FtsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	92,082	0	A	G	100.0%	126.3 / NA	36	T224A (ACT→GCT)	ftsI	peptidoglycan DD‑transpeptidase FtsI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/21);  total (15/21)

CTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTG  >  NC_000913/91952‑92207                                                                                                                                   |                                                                                                                              cTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGcc                                                                                                                      >  1:365249/1‑140 (MQ=255)     aCTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGc                                                                                                                  <  1:318128/140‑1 (MQ=255)           gTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGca                                                                                                            <  1:285456/140‑1 (MQ=255)           gTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGca                                                                                                            <  2:146790/140‑1 (MQ=255)           gTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGca                                                                                                            <  2:552662/140‑1 (MQ=255)            tCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGcac                                                                                                           >  1:188560/1‑140 (MQ=255)                  gTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTg                                                                                                     <  2:289218/140‑1 (MQ=255)                  gTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTg                                                                                                     <  2:129663/140‑1 (MQ=255)                   tCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTgg                                                                                                    >  1:528950/1‑140 (MQ=255)                     aaGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGgcg                                                                                                  <  2:365249/140‑1 (MQ=255)                     aaGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGgcg                                                                                                  <  2:426549/140‑1 (MQ=255)                       gggATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCt                                                                                                >  1:376225/1‑140 (MQ=255)                       gggATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCt                                                                                                <  2:376225/140‑1 (MQ=255)                       gggATTGAGGGCGTTAAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCt                                                                                                <  2:300403/140‑1 (MQ=255)                            tGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTa                                                                                           >  2:632944/1‑140 (MQ=255)                            tGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTa                                                                                           >  2:658389/1‑140 (MQ=255)                                   gTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATtgatga                                                                                    >  1:531212/1‑140 (MQ=255)                                          agagTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTg                                                                             <  2:531212/140‑1 (MQ=255)                                            agTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCa                                                                           <  2:188560/140‑1 (MQ=255)                                              tttCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAgg                                                                         >  1:471806/1‑140 (MQ=255)                                                tCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGgcg                                                                       <  2:471806/140‑1 (MQ=255)                                                 cGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGgcgc                                                                      >  1:724356/1‑140 (MQ=255)                                                                   gCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGa                                                    <  1:658389/140‑1 (MQ=255)                                                                    cAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATc                                                             >  1:142845/1‑130 (MQ=255)                                                                    cAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATc                                                             <  2:142845/130‑1 (MQ=255)                                                                    cAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGaa                                                   >  2:358475/1‑140 (MQ=255)                                                                            tGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCgg                                           >  2:483728/1‑140 (MQ=255)                                                                            tGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCgg                                           <  1:358475/140‑1 (MQ=255)                                                                                 gcATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGcc                                      >  2:104899/1‑140 (MQ=255)                                                                                 gcATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGcc                                      <  1:483728/140‑1 (MQ=255)                                                                                        gCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACa                               <  1:632944/140‑1 (MQ=255)                                                                                        gCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACa                               >  1:396063/1‑140 (MQ=255)                                                                                           tAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAgg                            <  1:104899/140‑1 (MQ=255)                                                                                                aCCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGaa                       <  2:724356/140‑1 (MQ=255)                                                                                                          tCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTAGCCTTTAACAAGGCTGAATCTGGTAgcg             <  2:664839/140‑1 (MQ=255)                                                                                                                          atTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCtggtg  <  2:395555/135‑1 (MQ=255)                                                                                                                          atTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCtggtg  >  1:395555/1‑135 (MQ=255)                                                                                                                                   |                                                                                                                              CTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTG  >  NC_000913/91952‑92207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑